Anomalous thermal denaturing of proteins adsorbed to nanoparticles

Teichroeb, Jonathan H.; Forrest, James A.; Ngai, Valentina; Jones, Lyndon

doi:10.1140/epje/i2006-10040-2

Cited by 50 publications

(37 citation statements)

References 14 publications

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“…protein-NP conjugates. Although NMR [62,63] and optical spectroscopy [26][27][28][29][30][31][32][33][34][35][36] have been used to characterize the ensemble average behaviour of suspensions of protein-NP conjugates, our use of SMFS provides the first investigation of the properties of proteins adsorbed to NP surfaces at the single-molecule level. Moreover, previous studies of bulk suspensions of protein-NP conjugates have suffered from the presence of impurities because suspensions of NPs are typically stabilized by charge or surfactants before the introduction of proteins.…”

Section: Discussionmentioning

confidence: 99%

The effect of nanoscale surface curvature on the oligomerization of surface-bound proteins

Kurylowicz

Paulin

Mogyoros

et al. 2014

J. R. Soc. Interface.

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ReviewCite this article: Kurylowicz M, Paulin H, Mogyoros J, Giuliani M, Dutcher JR. 2014 The effect of nanoscale surface curvature on the oligomerization of surface-bound proteins. J. R. Soc. The influence of surface topography on protein conformation and association is used routinely in biological cells to orchestrate and coordinate biomolecular events. In the laboratory, controlling the surface curvature at the nanoscale offers new possibilities for manipulating protein-protein interactions and protein function at surfaces. We have studied the effect of surface curvature on the association of two proteins, a-lactalbumin (a-LA) and b-lactoglobulin (b-LG), which perform their function at the oil-water interface in milk emulsions. To control the surface curvature at the nanoscale, we have used a combination of polystyrene (PS) nanoparticles (NPs) and ultrathin PS films to fabricate chemically pure, hydrophobic surfaces that are highly curved and are stable in aqueous buffer. We have used single-molecule force spectroscopy to measure the contour lengths L c for a-LA and b-LG adsorbed on highly curved PS surfaces (NP diameters of 27 and 50 nm, capped with a 10 nm thick PS film), and we have compared these values in situ with those measured for the same proteins adsorbed onto flat PS surfaces in the same samples. The L c distributions for b-LG adsorbed onto a flat PS surface contain monomer and dimer peaks at 60 and 120 nm, respectively, while a-LA contains a large monomer peak near 50 nm and a dimer peak at 100 nm, with a tail extending out to 200 nm, corresponding to higher order oligomers, e.g. trimers and tetramers. When b-LG or a-LA is adsorbed onto the most highly curved surfaces, both monomer peaks are shifted to much smaller values of L c . Furthermore, for b-LG, the dimer peak is strongly suppressed on the highly curved surface, whereas for a-LA the trimer and tetramer tail is suppressed with no significant change in the dimer peak. For both proteins, the number of higher order oligomers is significantly reduced as the curvature of the underlying surface is increased. These results suggest that the surface curvature provides a new method of manipulating protein-protein interactions and controlling the association of adsorbed proteins, with applications to the development of novel biosensors.

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Section: Discussionmentioning

confidence: 99%

The effect of nanoscale surface curvature on the oligomerization of surface-bound proteins

Kurylowicz

Paulin

Mogyoros

et al. 2014

J. R. Soc. Interface.

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“…The size of the particles and their surface curvature, are key issues in the structure alteration, as well as the type of protein involved: globular or fibrillar [37]. BSA, being a globular protein, tends to maintain its structure when adsorbed on highly curved surfaces, preserving its functional properties [38,39]. This effect can be explained by a simple model: flat surfaces exhibit more contact area for adsorbed proteins, according to the side-on model for BSA [40].…”

Section: Interaction With Bsamentioning

confidence: 99%

Effects of size and surface functionalization of zinc oxide (ZnO) particles on interactions with bovine serum albumin (BSA)

Simonelli

Arancibia

2015

Journal of Molecular Liquids

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“…They also play an important role in new interesting physics phenomena such as Surface Enhanced Raman Spectroscopy (SERS), Surface Plasmon Energy Transfer (SET), and Forster Resonance Energy Transfer (FRET) and have numerous other applications in plasmonics and nanotechnology and medical treatments [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Second quantization model for surface plasmon polariton in metallic nano wires

Lan

Nga²,

Việt³

2016

J. Phys.: Conf. Ser.

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Abstract. A model of effective Hamiltonian is proposed in second quantization representation for system of surface plasmons and photon (polariton) in metallic nano wires. The dispersion relation curves of surface plasmon polariton was calculated by mean of the Bogoliubov diagonalization method. The surface plasmon photon vertexes are considered. The conditions for excitation surface plasmon, existence plasmon radiate modes, and a possible application of metallic nano wires were also discussed. IntroductionIn the last tens years, the new field of plasmon has been developed, which discussed the interesting features of metals with nano structure, exciting with an electromagnetic radiation: the plasmon resonance.The plasmon is a quasiparticle resulting from the quantization of plasma oscillations. Plasmons are collective oscillations of the free electron gas density. A Plasmon can couple with a photon to create another quasiparticle called a plasmon polariton. Surface plasmons are plasmons which are restricted in surfaces and interact strongly with light resulting in a polariton. They occur in the interface of a vacuum and material with a small positive imaginary and large negative real dielectric constant. It is usually a metal or doped dielectric.The result of this combination is the interesting optical properties of absorption and scatter in the Vis -IR region and can be exploited for many applications in modern technology. They also play an important role in new interesting physics phenomena such as Surface Enhanced Raman Spectroscopy (SERS), Surface Plasmon Energy Transfer (SET), and Forster Resonance Energy Transfer (FRET) and have numerous other applications in plasmonics and nanotechnology and medical treatments [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16].Since for all these surface plasmon polariton (SPP) systems the downscaling of the cross section is not limited by the light wavelength, they represent a promising alternative to dielectric optical waveguides. Thus, there is a substantial interest in the fundamental properties of SPP propagation in nanoscale structured matter, which is determined by the respective dispersion relations.Nowadays, the experimental determination of the dispersion relation in the confined metallic films with nanoscopic cross sections nanowires is a challenging task, as the methods such as total internal reflective attentuated spectroscopy cannot be applied easily because of the small size

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Anomalous thermal denaturing of proteins adsorbed to nanoparticles

Cited by 50 publications

References 14 publications

The effect of nanoscale surface curvature on the oligomerization of surface-bound proteins

The effect of nanoscale surface curvature on the oligomerization of surface-bound proteins

Effects of size and surface functionalization of zinc oxide (ZnO) particles on interactions with bovine serum albumin (BSA)

Second quantization model for surface plasmon polariton in metallic nano wires

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