Anopheles (Kerteszia) cruzii infected by Plasmodium in the Atlantic Forest indicates that the malaria transmission cycle is maintained even after howler monkeys’ population decline

Ferreira, Lucas Mendes; Rezende, Helder Ricas; Fux, Blima; Alencar, Filomena Eurídice Carvalho de; Loss, Ana Carolina; Buery, Julyana Cerqueira; Duarte, Ana Maria Ribeiro de Castro; Cerutti, Crispim

doi:10.1007/s00436-022-07689-z

Cited by 4 publications

(3 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…and infect Kerteszia in the absence of howler monkeys. In agreement with this view is another study undertaken on the slopes of Serra do Mar in Espírito Santo State where anopheline infection rates were unaffected by yellow fever outbreaks and local Alouatta population decline [39]. We hypothesize that one or two of the remaining simian species in the Legado das Águas Forest Reserve can play a role as alternative reservoirs.…”

Section: Discussionsupporting

confidence: 81%

Zoonotic Malaria Risk in Serra Do Mar, Atlantic Forest, Brazil

Rangel,

Duarte,

Oliveira

et al. 2023

Microorganisms

Self Cite

View full text Add to dashboard Cite

Here, the main goal is to assess natural infections of Plasmodium spp. in anophelines in a forest reserve from the same region where we previously found a surprisingly high rate (5.2%) of plasmodia infections (n = 25) in Kerteszia mosquitoes (N = 480) on the slopes of Serra do Mar, Atlantic Forest, Brazil. The mosquito collection sampling was carried out at the Legado das Águas Forest Reserve using CDC light traps and Shannon traps at night (5–10 pm) in 3-day collections in November 2021 and March, April, May, and November 2022. The captured specimens were morphologically identified at the species level and had their genomic DNA extracted in pools of up to 10 mosquitoes/pool. Each pool was tested using 18S qPCR and cytb nested PCR plus sequencing. A total of 5301 mosquitoes, mostly belonging to the genus Kerteszia (99.7%), were sampled and sorted into 773 pools. Eight pools positive for Plasmodium spp. were identified: four for Plasmodium spp., one for P. vivax or P. simium, one for P. malariae or P. brasilianum, and two for the P. falciparum-like parasite. After Sanger sequencing, two results were further confirmed: P. vivax or P. simium and P. malariae or P. brasilianum. The minimum infection rate for Kerteszia mosquitoes was 0.15% (eight positive pools/5285 Kerteszia mosquitoes). The study reveals a lower-than-expected natural infection rate (expected = 5.2% vs. observed = 0.15%). This low rate relates to the absence of Alouatta monkeys as the main simian malaria reservoir in the studied region. Their absence was due to a significant population decline following the reemergence of yellow fever virus outbreaks in the Atlantic Forest from 2016 to 2019. However, this also indicates the existence of alternative reservoirs to infect Kerteszia mosquitoes. The found zoonotic species of Plasmodium, including the P. falciparum-like parasite, may represent a simian malaria risk and thus a challenge for malaria elimination in Brazil.

show abstract

Section: Discussionsupporting

confidence: 81%

Zoonotic Malaria Risk in Serra Do Mar, Atlantic Forest, Brazil

Rangel,

Duarte,

Oliveira

et al. 2023

Microorganisms

Self Cite

View full text Add to dashboard Cite

show abstract

“…Notably, An. cruzii mosquitoes are considered the primary vectors of transmission for humans and simian malaria in the Brazilian regions covered by the Atlantic Rainforest [ 55 , 56 , 57 ]; however, Anopheles spp. are known to be competent vectors of transmission for the o’nyong nyong virus (ONNV), an arbovirus endemic in East Africa [ 58 ] and possibly Cacipacore virus (CPCV), a zoonotic arbovirus endemic to Brazil [ 29 , 59 ].…”

Section: Experimental Studies On Ecology and Transmission Cyclesmentioning

confidence: 99%

Exploring Iguape Virus—A Lesser-Known Orthoflavivirus

Saivish,

Nogueira,

Rossi

et al. 2024

Viruses

View full text Add to dashboard Cite

Brazil has earned the moniker “arbovirus hotspot”, providing an ideal breeding ground for a multitude of arboviruses thriving in various zoonotic and urban cycles. As the planet warms and vectors expand their habitat range, a nuanced understanding of lesser-known arboviruses and the factors that could drive their emergence becomes imperative. Among these viruses is the Iguape virus (IGUV), a member of the Orthoflavivirus aroaense species, which was first isolated in 1979 from a sentinel mouse in the municipality of Iguape, within the Vale do Ribeira region of São Paulo State. While evidence suggests that IGUV circulates among birds, wild rodents, marsupials, bats, and domestic birds, there is no information available on its pathogenesis in both humans and animals. The existing literature on IGUV spans decades, is outdated, and is often challenging to access. In this review, we have curated information from the known literature, clarifying its elusive nature and investigating the factors that may influence its emergence. As an orthoflavivirus, IGUV poses a potential threat, which demands our attention and vigilance, considering the serious outbreaks that the Zika virus, another neglected orthoflavivirus, has unleashed in the recent past.

show abstract

“…Encontro semelhante ocorreu em Santa Teresa, no Espírito Santo onde a presença de DNA de P. falciparum e P. malariae / P. brasilianum foi detectada em fezes de Sapajus nigritus de vida livre (FERREIRA et al, 2022) 1.4 Ambiente Mudanças no uso da terra, métodos agrícolas, padrões residenciais e comportamento humano podem interferir nas dinâmicas de transmissão da malária, bem como precipitação, temperatura e outras variáveis climáticas (GUBLER et al, 2001).…”

Section: Hospedeirosunclassified

Investigação sobre a presença de Plasmodium spp. em Anophelinae (Diptera: Culicidae) do Vale do Ribeira, sudeste da Mata Atlântica, Estado de São Paulo, Brasil

Rangel

View full text Add to dashboard Cite

Agradecimentos À professora Dra. Maria Anice Mureb Sallum, por todo o aprendizado e oportunidades promovidas. À Dra. Tatiane Marques Porangaba, por toda paciência, aprendizado, ajuda com as análises e suporte no laboratório. À professora Dra. Ana Maria Duarte, por todo cuidado, aprendizado e ajuda com as análises, bem como por disponibilizar seu laboratório para as análises e extração de DNA enquanto o laboratório da FSP passava por reformas e readequação. Ao Professor Dr. Gabriel Zorello Laporta, pelas oportunidades, ajuda nas coletas e por todo aprendizado. À Raquel Montes e Luísa Kelly, por toda ajuda com as extrações e procedimentos. Ao meu marido, Ricardo Rangel, por toda paciência e apoio todos estes anos. À organização e participantes do XXV seminário Laveran e Deane sobre Malária, por todas as contribuições. Aos técnicos da antiga SUCEN: Bonafé, João, Valdomiro e os motoristas Fortunato e Sergio, pelo companheirismo e todo aprendizado de campo. À reserva Votorantim -Legado das Águas, pela concessão do local de pesquisa, cessão de guias para as trilhas, e toda a infraestrutura que foi crucial para o trabalho. Aos funcionários da reserva Votorantim -Legado das Águas, por todo carinho e ajuda. Aos moradores do Ribeirão da Anta, por todo acolhimento, carinho e comidas gostosas. O presente trabalho foi realizado com apoio da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior -Brasil (CAPES) -Código de Financiamento 001. RANGEL, M. E. O. Investigação da presença de Plasmodium spp. em Anophelinae (Diptera: Culicidae) do Vale do

show abstract

Anopheles (Kerteszia) cruzii infected by Plasmodium in the Atlantic Forest indicates that the malaria transmission cycle is maintained even after howler monkeys’ population decline

Cited by 4 publications

References 31 publications

Zoonotic Malaria Risk in Serra Do Mar, Atlantic Forest, Brazil

Zoonotic Malaria Risk in Serra Do Mar, Atlantic Forest, Brazil

Exploring Iguape Virus—A Lesser-Known Orthoflavivirus

Investigação sobre a presença de Plasmodium spp. em Anophelinae (Diptera: Culicidae) do Vale do Ribeira, sudeste da Mata Atlântica, Estado de São Paulo, Brasil

Contact Info

Product

Resources

About