The purpose of the study was to identify the effect of organizational culture on value creation with the mediating role of strategic alignment of knowledge management in Shahrvand Commodity Services Company. According to the topic, it is clear that this research is a descriptive-analytical study with applied design. The population was Tehran Shahrvand Commodity Services Company, with the number of samples as 225 personnel according to Morgan Table . Data collection tools were questionnaires and interviews. Moreover, QI Corporate Innovation Questionnaire was used to measure value creation in 2013 in Iran. Additionally, Lawson's Knowledge Management Assessment Instrument (KMAI), developed in 2003, and the researcher-developed organizational culture questionnaire, whose validity had been proven was used. Data analysis was done using structural equation modeling (SEM) and PLS software with the results showing that the indirect relationship between organizational culture and organizational innovation through strategic alignment is insignificant and the hypothesis was not approved.