18Recent call has been made to study the biogeography of species interactions in order to better 19 understand ecosystems' states and processes, as well as their response to global anthropogenic 20 disturbances. Ants (Formicidae) are a dominant group of arthropods with a central role in 21 ecosystem functioning. Many ant species, those feeding on liquids, are floricolous and consume 22 nectar. The biogeography of ant-flower interactions is still poorly studied and especially in 23 temperate area. Here we quantify variations in ant-flower interaction frequency in response to 24 latitudinal and urbanization gradients at a country scale. 25 We used data from a flower-visitor monitoring program that includes pictures on 2511 flower 26 plants across Continental France (Mainland) and over 4 years. We analysed the occurrence of 27 the ant-flower interactions along two gradients: latitude and urbanization, this for 10 ant taxa 28 corresponding to different taxonomical level (from family to species). 29 Ants visited 26 % of the sampled plants. Most of the observed ant-flower interaction involved 30 the subfamily Formicinae (82.1 %), followed by Myrmicinae and Dolichoderinae (6.9 % and 31 4.6 % respectively). Globally, (i) the probability of occurrence was negatively related to 32 latitudes (ii) and to urbanization at lower latitude. (iii) Responses to latitude among sub families, 33 genus and species level responses were segregated and taxonomically aggregated. 34 At lower taxonomic levels we found clear latitudinal niche partitioning among ant taxa 35 suggesting that competition, on both evolutionary and ecological time scales, is a major process 36 structuring ant communities. Finally, our results highlight that the effects of large scale 37 perturbation like urbanization can vary and affect latitudinal gradient. 38 Science; Inter-specific interaction; Niche partitioning 40 41 42 2008, Antonova and Penev 2006, Forys and Allen 2005, Holway and Suarez 2006, Lessard and 65 Buddle 2005, López-Moreno , Majer and Brown 1986, Sanford et al. 2009, 66 Thompson and McLachlan 2007, Uno et al. 2010, Xu et al. 1998 and one study a positive 67 effect (Ives et al. 2011). The effects of urbanization on ant's abundance have been less studied 68 130 Urbanization was characterized by the artificial area category of the first level of Corinne Land 131 Cover 2006 database (raster version 25 m resolution, EEA 2010). In France, urbanization is 132 Distrib. 17: 652-662. 388