Antagonism between C/EBPβ and FOG in eosinophil lineage commitment of multipotent hematopoietic progenitors

Querfurth, Erich; Schuster, Mikkel Bruhn; Kulessa, Holger; Crispino, John D.; Döderlein, Gabriele; Orkin, Stuart H.; Graf, Thomas; Nerlov, Claus

doi:10.1101/gad.177200

Cited by 113 publications

(94 citation statements)

References 56 publications

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“…The ectopic expression of PU.1 and C/EBP also results in the commitment of hematopoietic progenitors to an eosinophilic lineage. [18][19][20] With respect to normal primary cells, it has been reported that murine B-cell precursor cells acquire the potential to differentiate into macrophage-like cells when they are transferred to myeloid culture conditions. 21 Normal murine early T progenitor cells also generate macrophages in cultures supplemented with conditioned medium from a thymic stromal cell line or in the presence of cytokines.…”

Section: Introductionmentioning

confidence: 99%

Reprogramming of human postmitotic neutrophils into macrophages by growth factors

Araki

Katayama

Yamashita

et al. 2004

Blood

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It is generally recognized that postmitotic neutrophils give rise to polymorphonuclear neutrophils alone. We obtained evidence for a lineage switch of human postmitotic neutrophils into macrophages in culture. When the CD15 ؉ CD14 ؊ cell population, which predominantly consists of band neutrophils, was cultured with granulocyte macrophage-colonystimulating factor, tumor necrosis factor-␣, interferon-␥, and interleukin-4, and subsequently with macrophage colonystimulating factor alone, the resultant cells had morphologic, cytochemical, and phenotypic features of macrophages. In contrast to the starting population, they were negative for myeloperoxidase, specific esterase, and lactoferrin, and they upregulated nonspecific esterase activity and the expression of macrophage colony-stimulating factor receptor, mannose receptor, and HLA-DR. CD15 ؉ CD14 ؊ cells proceeded to macrophages through the CD15 ؊ CD14 ؊ cell population. Microarray analysis of gene expression also disclosed the lineage conversion from neutrophils to macrophages. Macrophages derived from CD15 ؉ CD14 ؊ neutrophils had phagocytic function. Data obtained using 3 different techniques, including Ki-67 staining, bromodeoxyuridine incorporation, and cytoplasmic dye labeling, together with the yield of cells, indicated that the generation of macrophages from CD15 ؉ CD14 ؊ neutrophils did not result from a contamination of progenitors for macrophages. Our data show that in response to cytokines, postmitotic neutrophils can become macrophages. This may represent another differentiation pathway toward macrophages in human postnatal

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Section: Introductionmentioning

confidence: 99%

Reprogramming of human postmitotic neutrophils into macrophages by growth factors

Araki

Katayama

Yamashita

et al. 2004

Blood

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“…65 Surprisingly, in eosinophil and mast cells, FOG-1 can antagonize GATA-1 activity thereby functioning as a corepressor within this cellular-dependent context. 62,66 The widely explored model of cell fate decisions during hematopoietic stem and progenitor development is based on the cross-antagonistic relationship between the PU.1 and GATA-1 transcription factors. However, once multi-potential progenitors differentiate into latter precursor stages such as monocyte/ granulocyte development, GATA-1 is no longer expressed and the process of cell specification depends on PU.1 and another primary lineage determinant, CEBPa.…”

Section: Pu1 and Gata-1 Interact During Early Hematopoietic Cell Decmentioning

confidence: 99%

The role of PU.1 and GATA-1 transcription factors during normal and leukemogenic hematopoiesis

2010

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Hematopoiesis is coordinated by a complex regulatory network of transcription factors and among them PU.1 (Spi1, Sfpi1) represents a key molecule. This review summarizes the indispensable requirement of PU.1 during hematopoietic cell fate decisions and how the function of PU.1 can be modulated by protein-protein interactions with additional factors. The mutual negative regulation between PU.1 and GATA-1 is detailed within the context of normal and leukemogenic hematopoiesis and the concept of 'differentiation therapy' to restore normal cellular differentiation of leukemic cells is discussed.

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“…A number of studies suggest that combinatorial interactions between hematopoietic factors regulate lineage selection. For example, Friend of GATA (FOG)-1 converts GATA-1 from an activator to a repressor of eosinophil lineage commitment (1). In addition, this complex is required for erythrocyte and megakaryocyte differentiation (2)(3)(4).…”

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confidence: 99%

Combinatorial interactions of Serpent, Lozenge, and U-shaped regulate crystal cell lineage commitment during Drosophila hematopoiesis

Fossett

Hyman

Gajewski

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

120

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The GATA factor Serpent (Srp) is required for hemocyte precursor formation during Drosophila hematopoiesis. These blood cell progenitors give rise to two distinct lineages within the developing embryo. Lozenge, a Runx protein homologue, and Glial cells missing-1 and -2 are essential for crystal cell and plasmatocyte production, respectively. In contrast U-shaped, a Friend of GATA class factor, antagonizes crystal cell formation. Here we show that Srp, Lozenge, and U-shaped interact in different combinations to regulate crystal cell lineage commitment. Coexpression of Srp and Lozenge synergistically activated the crystal cell program in both embryonic and larval stages. Furthermore, expression of Lozenge and SrpNC, a Srp isoform with N-and C-terminal zinc fingers, inhibited u-shaped expression, indicating that crystal cell activation coincided with the down-regulation of this repressor-encoding gene. In contrast, whereas U-shaped and SrpNC together blocked crystal cell production, coexpression of U-shaped with noninteracting Srp proteins failed to prevent overproduction of this hemocyte population. Such results indicated that U-shaped and SrpNC must interact to block crystal cell production. Taken together, these studies show that the specialized SrpNC isoform plays a pivotal role during crystal cell lineage commitment, acting as an activator or repressor depending on the availability of specific transcriptional coregulators. These findings provide definitive proof of the combinatorial regulation of hematopoiesis in Drosophila and an in vivo demonstration of GATA and Runx functional interaction in a blood cell commitment program.

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Antagonism between C/EBPβ and FOG in eosinophil lineage commitment of multipotent hematopoietic progenitors

Cited by 113 publications

References 56 publications

Reprogramming of human postmitotic neutrophils into macrophages by growth factors

Reprogramming of human postmitotic neutrophils into macrophages by growth factors

The role of PU.1 and GATA-1 transcription factors during normal and leukemogenic hematopoiesis

Combinatorial interactions of Serpent, Lozenge, and U-shaped regulate crystal cell lineage commitment during Drosophila hematopoiesis

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