Antagonism of Helicobacter pylori by Bacteriocins of Lactic Acid Bacteria

Abstract

Antimicrobial activity of seven bacteriocins produced by lactic acid bacteria against Helicobacter pylori strains (ATCC 43504, Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH [DSM] 4867, DSM 9691, and DSM 10242) was investigated in vitro using a broth microdilution assay. The bacteriocins chosen for the study were nisin A; lacticins A164, BH5, JW3, and NK24; pediocin PO2; and leucocin K. Antimicrobial activity of the bacteriocins varied among the H. pylori strains tested, of which strain ATCC 43504 was the most tolerant. Among the bacteriocins tested, lacticins A164 and BH5 produced by Lactococcus lactis subsp. lactis A164 and L. lactis BH5, respectively, showed the strongest antibacterial activity against H. pylori strains. MICs of the lacticins against H. pylori strains, when assessed by the critical dilution micromethod, ranged from 0.097 to 0.390 mg/liter (DSM strains) or from 12.5 to 25 mg/liter (ATCC 43504), supporting the strain-dependent sensitivity of the pathogen. Pediocin PO2 was less active than the lacticins against four strains of H. pylori, and leucocin K was the least active peptide, with no inhibition toward H. pylori ATCC 43504. Anti-Helicobacter activity of lacticin A164 was dependent on initial inoculum size as well as concentration of the bacteriocin added.

Anti-Infective Activities of Lactobacillus Strains in the Human Intestinal Microbiota: from Probiotics to Gastrointestinal Anti-Infectious Biotherapeutic Agents

“…It has been documented that the human probiotic intestinal microbiota L. johnsonii NCC 533, L. casei Shirota, and L. acidophilus LB display direct antagonistic activity in vitro against the gastritisassociated bacterium H. pylori ( Fig. 1 (113,(152)(153)(154). The molecule(s) present in L. acidophilus LB CFCS that exerts bactericidal activity against H. pylori remain to be identified.…”

“…The generally held view is that bacteriocins exhibit less potential as chemotherapeutics for infections with Gram-negative pathogens. However, it should be noted that several bacteriocins and bacteriocin-like molecules produced by probiotic Lactobacillus strains have been also found to be active against the growth of several Gram-negative gastric or enterovirulent bacterial pathogens, including H. pylori, EHEC, Shigella, Salmonella, and Campylobacter (112)(113)(114)(115)(116).…”

Systematic review: are probiotics useful in controlling gastric colonization by Helicobacter pylori?

“…Kim et al 28 evaluated, by microdilution assay, the anti-H. pylori activity of seven bacteriocins produced by lactic acid bacteria, including nisin A, pediocin PO2, leucocin K and various types of lacticins. Lacticins A164 and BH5 produced by strains of L. lactis had the most potent activity, with minimum inhibitory concentrations varying from 0.097-0.390 mg/L to 12.5-25 mg/L depending on the strains of H. pylori tested, indicating a strain-dependent sensitivity of this pathogen.…”

Evaluation of the anti-Helicobacter pylori and cytotoxic properties of the antimicrobial substances from Lactobacillus acidophilus BK13 and Lactobacillus paracasei BK57

“…responsible for the inhibitory activity. Kim et al (2003) suggested that lacticins A164 and BH5 produced by Lactococcus lactis subsp. lactis A164 and L. lactis BH5, respectively, showed the anti-H. pylori activity of lacticin A164 was dependent on initial inoculum size as well as concentration of the bacteriocin added.…”