Antagonism of platelet activating factor‐induced chemiluminescence in guinea‐pig peritoneal macrophages in differing states of activation

Parnham, Michael J.; Bittner, Christine; Lambrecht, Günter

doi:10.1111/j.1476-5381.1989.tb12631.x

Cited by 4 publications

(1 citation statement)

References 20 publications

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“…44 All antagonists produced a shift to the right in the dose-response curve to PAF. Schild plots for BN 52021, L-652,731, kadsurenone, and SRI 63-119 were linear, but only for BN 52021 and kadsurenone did the mean slope not differ significantly from unity.…”

Section: A Paf Receptors On Macrophagesmentioning

confidence: 95%

Recent progress in ginkgolide research

Braquet

Esanu²,

Buisine

et al. 1991

Medicinal Research Reviews

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Section: A Paf Receptors On Macrophagesmentioning

confidence: 95%

Recent progress in ginkgolide research

Braquet

Esanu²,

Buisine

et al. 1991

Medicinal Research Reviews

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Platelet-activating factor–induced calcium mobilization and oxidative metabolism in hepatic macrophages and endothelial cells

Gardner

Laskin

1993

Journal of Leukocyte Biology

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Fluorescence image analysis of the calcium sensitive dye Indo-1 was used to characterize platelet-activating factor (PAF)-induced calcium mobilization in hepatic macrophages and endothelial cells. PAF, but not lyso-PAF, an inactive analog, induced a rapid and transient increase in intracellular levels of calcium that appeared to depend on the presence of extracellular calcium. In both macrophages and endothelial cells, these effects were dose dependent, reaching maximal levels with 10 nM PAF. However, the kinetics of the calcium response to PAF in macrophages and endothelial cells was distinct. The endothelial cells responded more rapidly to PAF than the macrophages (within 1 min vs. 2-3 min, respectively) and displayed longer recovery periods (4 min vs. > 10 min, respectively). In contrast, the magnitude of the response to PAF was greater in the macrophages. In both cell types, triazolam and alprazolam, two PAF antagonists, and the calcium channel blocker verapamil inhibited PAF-induced calcium mobilization. PAF also stimulated superoxide anion production alone and in combination with the macrophage activator 12-O-tetradecanoyl-phorbol-13-acetate (TPA) in both cell types. Macrophages produced more of this reactive oxygen intermediate in response to PAF and TPA than endothelial cells. Interestingly, in the absence of extracellular calcium or when verapamil was added to the cultures, superoxide anion production by the macrophages, but not the endothelial cells, was significantly reduced. These results demonstrate that, although hepatic macrophages and endothelial cells mobilize calcium in response to PAF, the characteristics of the response in each cell type are different. These differences may underlie, at least in part, distinct functional responses of the cells to PAF.

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Biological Properties of Platelet-Activating Factor

Stewart¹

1994

Lipid Mediators

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Antagonism of platelet activating factor‐induced chemiluminescence in guinea‐pig peritoneal macrophages in differing states of activation

Cited by 4 publications

References 20 publications

Recent progress in ginkgolide research

Recent progress in ginkgolide research

Platelet-activating factor–induced calcium mobilization and oxidative metabolism in hepatic macrophages and endothelial cells

Biological Properties of Platelet-Activating Factor

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