Antagonist effect of Bacillus spp. against Aspergillus niger CP isolated from cocopeat powder

Abstract

Bacillus spp. are known as potential bacteria as biocontrol agent against fungal phytopathogen, such as Aspergillus niger. The Aspergillus niger can cause many problems, including in agriculture sector. Antagonist activity of 3 bacteria isolates Bacillus sp. KRG, KRT and LDR have been carried out against Aspergillus niger CP. Dual culture method for antagonist assay was performed using streak, disc, and pour plate technique. Result from the disc technique showed that there is no significant antagonist activity differences between all isolates KRG, KRT, and LDR (51.94 %, 61.24 %, and 54.53 %). The highest inhibition effect was shown clearly in pour plate technique with inhibition value almost 100 %. The LDR isolate was selected for further evaluation based on the physicochemical characters according to strong catalase activity and rapid fermentation. Antibiosis assay was performed in order to evaluate the antifungal compound produced by LDR isolate using agar and broth culture method. The filtrates from growth medium 7, 10 and 12 days fermentation were used for antibiosis assay against Asp. niger CP. The growth of Asp. niger CP was inhibited by 10- and 12-days filtrate fermentation in all the 2 methods used. The percentage of inhibition of isolates observed in agar culture were 84.41 % and 84.21 %. Biomass of Asp. niger CP reduced by 74.55 % and 85.54 %.

Identification and characterization of Aspergillus niger causing collar rot of groundnut (Arachis hypogaea)

“…The similar specifications of Aspergillus niger was reported by (Gautam and Bhadauria 2012;Kasfi et al 2018;Romsdahl et al 2018;Rodriguez et al 2019;Kumar et al 2020;Šimonovicová et al 2021). Previously, Santoso et al (2021) identified A. niger based on cultural and morphological characteristics, such as black colony, hyaline conidiophore and sterigmata, biseriate conidia. Mahammed and Chala (2014) examined Aspergillus species differentiation based on their cultural features like colony color, texture and margins, as well as the size of conidia and conidiophores.…”

Genetically Modified Plants Based on Bacillus Genes and Commercial Bacillus-Based Biopesticides for Sustainable Agriculture

“…Bacillus strains have been intensively used against Fusarium [15][16][17][18][19] and Aspergillus [20][21][22] species. Also, Bacillus-based biocontrol has been used to decrease mycotoxin contamination in crops [23][24][25].…”

Inhibition of the phytopathogenic fungi Curvularia lunata BM and Ganoderma sp. TB4 by antifungal compounds produced by Bacillus siamensis LDR grown on hanjeli (Coix lacryma-jobi L.) starch

“…In the present research, B. siamensis LDR isolated from coconut peat was evaluated for its antifungal compounds using an antibiosis assay (Santoso et al 2021a). This Bacillus species has shown antagonistic activity against several fungal pathogens, such as Aspergillus niger (Santoso et al 2021a), Colletotrichum siamense KA (Fadhilah et al 2021), Fusarium sp., Chaetomium globosum InaCC F228, Ganoderma sp. TB 4 (Santoso et al 2021b), A. clavatus, A. flavus, A. tamarii (Pertiwi et al 2021), and C. lunata BM (Safitri et al 2021.…”