Antagonistic Activities and Probiotic Potential of Lactic Acid Bacteria Derived From a Plant-Based Fermented Food

Abstract

In this study, antagonistic activities and probiotic potential of lactic acid bacteria (LAB) derived from a plant-based fermented food, kimchi, were demonstrated. The cell free supernatants (CFS) from Lactobacillus curvatus KCCM 43119, Leuconostoc mesenteroides KCCM 43060, Weissella cibaria KCTC 3746, and W. koreensis KCCM 41517 completely inhibited the growth of foodborne pathogenic bacteria, while neutralized CFS (pH 6.5) partially inhibited the growth. The competition, exclusion, and displacement of foodborne pathogenic bacteria by the LAB strains from adhesion to HT-29 cells were investigated. The LAB strains were able to compete with, exclude, and displace the foodborne pathogenic bacteria. However, the degree of inhibition due to the adhesion was found to be a LAB strain-dependent phenomenon. The LAB strains showed high coaggregation with foodborne pathogenic bacteria, and they also exhibited high resistance to acidic condition. Except W. cibaria KCTC 3746, all LAB strains were capable of surviving in the presence of bile salts. Furthermore, while all LAB strains were resistant to chloramphenicol, kanamycin, streptomycin, gentamicin, and erythromycin, only W. cibaria KCTC 3746 and W. koreensis KCCM 41517 displayed resistance to vancomycin. These results suggest that the LAB strains derived from kimchi exerted antagonistic activities against foodborne pathogenic bacteria with probiotic potential.

Biological Control of Root-Knot Nematodes by Organic Acid-Producing Lactobacillus brevis WiKim0069 Isolated from Kimchi

“…However, of the four main organic acids produced by Wi-Kim0069, acetic acid exhibited the strongest nematicidal activity against M. incognita J2 in vitro . Thus, our findings indicate that acetic acid may be a key nematicidal factor for RKN management, unlike the results of a previous study (Choi et al, 2018). …”

“…Recent studies have revealed that LAB can inhibit infection by phytopathogenic and spoilage bacteria and fungi, such as Xanthomonas campestris , Erwinia carotovora , Aspergillus flavus , Colletotrichum capsici , Penicillium expansum , Monilinia laxa , and Botrytis cinerea , and inhibit mycotoxin production (El-Mabrok et al, 2012; Ghazvini et al, 2016; Khanafari et al, 2007; Trias et al, 2008). Given the antagonistic activity of LAB against pathogenic microorganisms, the industrial application of LAB as a biocontrol microbe could be an effective strategy for phytopathogen management (Choi et al, 2018; Trias et al, 2008). …”

“…The acidification of plant tissue can reduce the postharvest decay caused by Penicillium expansum and Alternaria alternate (Prusky et al, 2006). Furthermore, Choi et al (2018) proposed that organic acids, especially lactic acid, may be more closely associated with the antagonistic activity of the LAB strains than other known antimicrobial compounds. However, of the four main organic acids produced by Wi-Kim0069, acetic acid exhibited the strongest nematicidal activity against M. incognita J2 in vitro .…”

Characterization, health benefits and applications of fruits and vegetable probiotics

“…Some of the probiotics isolate have shown antagonistic towards plant pathogens. Several species of LAB were reported to (Chen et al, 2013) produce a wide array of antagonistic metabolites to FV pathogens as well as spoilage microbes (Choi, Patra, Kim, & Kang, 2018;Gajbhiye & Kapadnis, 2016). This is due to the synthesis of various primary as well as secondary metabolites including organic acids (acetic and lactic acids), carbon dioxide, ethanol, hydrogen peroxide, acetaldehyde, acetoin, antifungal compounds, free fatty acids, phenyl-lactic acids, hydroxyphenyl acids, bacteriocins (nisin), and antibiotics (reutericyclin), all of which may affect broad spectrum of fungi, bacteria, virus, and protozoa (Shokryazdan et al, 2017;Stanton, Ross, Fitzgerald, & Sinderen, 2005).…”

Characterization and in vitro properties of Lactobacillus plantarum and Leuconostoc mesenteroides for probiotic potential and nitrite degradation

“…Antibiotic resistance for LAB was determined as described previously 18,19 . Exactly, the following 8 antimicrobial agents (Shanghai Ryon Biological Technology Co., Ltd) were used: ampicillin, chloram-phenicol, streptomycin, tetracycline, erythromycin, clindamycin, norfloxacin, and vancomycin.…”