Antagonistic Activity of Lactic Acid Bacteria Lactobacillus spp. against Clinical Isolates of Klebsiella pneumoniae

Федорова, Т. В.; Vasina, Daria V.; Begunova, A.V.; Rozhkova, I.V.; Raskoshnaya, T.A.; Ni, Gabriélian

doi:10.1134/s0003683818030043

Cited by 17 publications

(5 citation statements)

References 17 publications

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“…In our previous work, we demonstrated that Lactobacillus helveticus NK1, Lactobacillus rhamnosus F and Lactobacillus reuteri LR1 strains exhibit good safety and technological performance, as well as provide good probiotic properties to the final products [ 11 , 12 ]. In this article, the proteolytic activity of these LABs and their ability to release bioactive peptides with antioxidant and angiotensin I-converting enzyme inhibitory (ACE-I) properties were investigated.…”

Section: Introductionmentioning

confidence: 99%

Development of Antioxidant and Antihypertensive Properties during Growth of Lactobacillus helveticus, Lactobacillus rhamnosus and Lactobacillus reuteri on Cow’s Milk: Fermentation and Peptidomics Study

Begunova

Savinova

Glazunova

et al. 2020

Foods

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Bioactive peptides derived from milk proteins are an active research area. Exhibiting numerous positive physiological effects on digestive, cardiovascular, immune and nervous systems, these peptides thought to be one of the most promising ingredients for functional food. Generally, these peptides are inactive within the parent proteins and can be liberated during milk fermentation by the specific proteolytic systems of various Lactobacillus spp. Here we present the study of milk fermentation by Lactobacillus helveticus NK1, Lactobacillus rhamnosus F and Lactobacillus reuteri LR1 strains. It was demonstrated that the antioxidant activity of the milk fermented by these strains concomitantly increased with the strains’ proteolytic activity. For the angiotensin I-converting enzyme (ACE) inhibitory activity, the same tendency was not observed. Although the proteolytic activity of L. helveticus NK1 was two times higher than that of L. rhamnosus F, the milk fermented by these strains showed comparable ACE inhibition. The analysis of the peptide profiles of the fermented milk samples allowed us to hypothesize that some previously unreported peptides can be produced by L. rhamnosus F. In addition, it was demonstrated that these potential ACE-inhibiting peptides originated from the C-terminus of αS2-casein.

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Section: Introductionmentioning

confidence: 99%

Development of Antioxidant and Antihypertensive Properties during Growth of Lactobacillus helveticus, Lactobacillus rhamnosus and Lactobacillus reuteri on Cow’s Milk: Fermentation and Peptidomics Study

Begunova

Savinova

Glazunova

et al. 2020

Foods

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“…At the same time, the antimicrobial potential of this extract is insufficient to suppress the proliferative activity of antibiotic resistant isolates of K. pneumoniae and L. amnigena. To date, a number of studies have been published, indicating the antimicrobial activity of L. reuteri metabolites obtained by cultivation of producer on conventional nutrient media [13,[25][26][27]. The results of these studies allow us to draw some conclusions: the main extracellular antibacterial agents of lactobacilli are organic acids (pH-neutralization elicited the most significant impact on the antimicrobial activity of cell-free supernatants, while proteinase and heat treatment had little or no impact); longer cultivation of the producer in microaerobic conditions results in stronger inhibition of test bacteria.…”

Section: Discussionmentioning

confidence: 99%

“…strains [13]. The suppression degree of the K. pneumoniae by L. reuteri varied in the range of 56-73 %.…”

Section: Original Researchmentioning

confidence: 99%

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Lactobacillus reuteri cell-free extracts against antibiotic-resistant bacteria

Knysh

Martynov

2020

ZMJ

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The aim of the research was to evaluate the antimicrobial potential of cell-free extracts obtained in various ways from the probiotic strain Lactobacillus reuteri DSM 17938 with respect to their ability to influence the proliferation of antibiotic-resistant bacteria.Materials and methods. Cell-free extracts were obtained: 1) from L. reuteri cell suspension, subjected to disintegration by repeated freezing-thawing, L; 2) from L. reuteri culture, cultivated in its own disintegrate (ML); 3) from L. reuteri culture, cultivated in its own disintegrate supplemented with glycerol (73.7 mg/ml) and glucose (72.1 mg/ml) (MLG); 4) from L. reuteri culture, cultivated in its own disintegrate supplemented with ascorbic acid (20 mg/ml) (MLA). Multidrug-resistant (MDR) and extensively drug-resistant (XDR) clinical isolates: Escherichia coli, Klebsiella pneumoniae, Lelliottia amnigena and Corynebacterium xerosis were used as a test cultures. The investigation of the inhibitory activity of cell-free extracts was carried out by spectrophotometric method using a microplate analyzer "Lisa Scan EM" ("Erba Lachema s.r.o.", Czech Republic).Results. Cell-free extract L exerted predominantly stimulatory effect on the proliferation of all studied test cultures. Cell-free extract ML had significant inhibitory effect on the proliferation of E. coli and C. xerosis (growth inhibition indices were 24.8 % and 96.1 %, respectively) and did not have significant effect on the proliferation of K. pneumoniae and L. amnigena. Cell-free extracts MLG and MLA caused pronounced inhibition of the proliferative activity of all tested microorganisms. Growth inhibition indices were: 75.0 % and 90.7 % (E. coli), 77.9 % and 88.9 % (K. pneumoniae), 40.9 % and 77.9 % (L. amnigena), 99 % and 100 % (C. xerosis), respectively.Conclusions. The cell-free extracts obtained by cultivation of L. reuteri DSM 17938 in its own disintegrate supplemented with glycerol and glucose or ascorbic acid have shown a pronounced antimicrobial activity against antibiotic-resistant bacteria in vitro. After confirming safety and efficacy in vivo, they can be used to increase the efficiency of the therapy of diseases caused by antibiotic-resistant microorganisms. The results of the study indicate the prospects of obtaining probiotic derivatives with high antimicrobial activity by applying a combinatorial (precursor directed) biosynthesis strategy.

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“…The strain was isolated from an endotracheal tube and its antibiotic resistance profile was determined with MicroScan WalkAway 96 plus system (Beckman Coulter, Atlanta, GA, USA) using MicroScan Neg Breakpoint Combo Panel Type 44 (NBC44). The strain demonstrated resistance to 24 antibiotics in the panel including: ampicillin, ampicillin/sulbactam, amoxi-cillin/clavulanic acid, piperacillin, piperacillin/tazobactam, cefazolin, cefuroxime, cefoxitin, cefotaxime, ceftazidime, ceftriaxone, cefepime, ciprofloxacin, levofloxacin, meropenem, imipenem (tienam), ertapenem, amikacin, gentamicin, tobramicine, aztreonam, tetracycline, tigecycline (tygacil), trimetoprim/sulfometoxazol [60].…”

Section: Bacterial Strains and Pre-cultivationsmentioning

confidence: 99%

Exoproteome Analysis of Antagonistic Interactions between the Probiotic Bacteria Limosilactobacillus reuteri LR1 and Lacticaseibacillus rhamnosus F and Multidrug Resistant Strain of Klebsiella pneumonia

Savinova

Glazunova

Moiseenko

et al. 2021

IJMS

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The expansion of multiple drug resistant (MDR) strains of Klebsiella pneumoniae presents an immense threat for public health. Annually, this microorganism causes thousands of lethal nosocomial infections worldwide. Currently, it has been shown that certain strains of lactic acid bacteria (LAB) can efficiently inhibit growth of K. pneumoniae and the formation of its biofilms; however, the active principle of such action remains unknown. In the current article, the growth inhibition of MDR K. pneumoniae by two LAB—Limosilactobacillus reuteri LR1 and Lacticaseibacillus rhamnosus F—is demonstrated, and the nature of this inhibition studied at the level of exoproteome. This article shows that the exoproteomes of studied LAB contains both classically and non-classically secreted proteins. While for L. reuteri LR1 the substantial portion of classically secreted proteins was presented by cell-wall-degrading enzymes, for L. rhamnosus F only one out of four classically secreted proteins was presented by cell-wall hydrolase. Non-classically secreted proteins of both LAB were primarily metabolic enzymes, for some of which a possible moonlighting functioning was proposed. These results contribute to knowledge regarding antagonistic interaction between LAB and pathogenic and opportunistic microorganisms and set new perspectives for the use of LAB to control the spread of these microorganisms.

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Antagonistic Activity of Lactic Acid Bacteria Lactobacillus spp. against Clinical Isolates of Klebsiella pneumoniae

Cited by 17 publications

References 17 publications

Development of Antioxidant and Antihypertensive Properties during Growth of Lactobacillus helveticus, Lactobacillus rhamnosus and Lactobacillus reuteri on Cow’s Milk: Fermentation and Peptidomics Study

Development of Antioxidant and Antihypertensive Properties during Growth of Lactobacillus helveticus, Lactobacillus rhamnosus and Lactobacillus reuteri on Cow’s Milk: Fermentation and Peptidomics Study

Lactobacillus reuteri cell-free extracts against antibiotic-resistant bacteria

Exoproteome Analysis of Antagonistic Interactions between the Probiotic Bacteria Limosilactobacillus reuteri LR1 and Lacticaseibacillus rhamnosus F and Multidrug Resistant Strain of Klebsiella pneumonia

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