Antagonistic activity of lipopeptide-biosurfactant producing Bacillus subtilis AKP, against Colletotrichum capsici, the causal organism of anthracnose disease of chilli

Kumar, Amrit; Rabha, Jintu; Jha, Dhruva Kumar

doi:10.1016/j.bcab.2021.102133

Cited by 21 publications

(11 citation statements)

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“…One of the mechanisms of this antagonistic activity is direct antagonism against pathogenic fungi by inducing disruption of the plasma membrane. Another mechanism was reported in the lipopeptide iturin and fengycin by affecting the surface tension of the fungal cell membrane, causing the formation of micropores and ion leakage, which then led to cell death (Kumar et al 2021). Antifungal activity is also influenced by the carbon source used in the biosurfactant production media.…”

Section: Discussionmentioning

confidence: 99%

Biosurfactant Activity of Bacillus sp. Strain LP04 Isolate and Its Antifungal Potency against Ganoderma boninense and Fusarium sp.

Maherani,

Mubarik,

Priyanto

et al. 2024

HAYATI J Biosci

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Biosurfactants are a class of amphipathic molecules that various microorganisms can produce. Biosurfactants are used as biopesticides and biocontrol agents because they have antimicrobial activity, especially as antifungal agents in several species of fungal pathogens such as Ganoderma boninense and Fusarium sp. that attack crops. This study aims to detect the biosurfactant activity of Bacillus sp. and its potential as an antifungal agent against the fungi Ganoderma boninense and Fusarium sp. Biosurfactants were produced in mineral salt medium (MSM) by harvesting cell-free supernatants. Screening of biosurfactant-producing isolates was carried out using an oil-spreading assay, a hemolysis assay, and an emulsification index. The antifungal activity of the isolates was then tested using the agar diffusion method. The LP04 isolate was closely related to Bacillus thuringiensis with a 99% similarity level. It has the potential to have biosurfactant activity, which is characterized by a positive result on the oil spreading assay test and has an emulsification index of 48.33±2.87%. The cell-free supernatants of the bacterial isolate were able to inhibit the growth of Ganoderma boninense and Fusarium sp. with growth inhibition rates of 51.11% and 56.92%, respectively.

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Section: Discussionmentioning

confidence: 99%

Biosurfactant Activity of Bacillus sp. Strain LP04 Isolate and Its Antifungal Potency against Ganoderma boninense and Fusarium sp.

Maherani,

Mubarik,

Priyanto

et al. 2024

HAYATI J Biosci

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“…In other previous studied cases, similar findings were reported where only one bacterial strain, Bacillus subtilis APK, exhibited significant antifungal potential against the anthracnose pathogen. This Bacillus strain demonstrated decreased pathogen mycelial growth in vitro and enhanced chili seedling growth under greenhouse conditions ( Kumar et al, 2021 ). Furthermore, a previous research has indicated that several Bacillus species can notably improve the growth and development of chili ( Peña-Yam et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Antagonistic activity of two Bacillus strains against Fusarium oxysporum f. sp. capsici (FOC-1) causing Fusarium wilt and growth promotion activity of chili plant

Iqbal,

Syed,

Rajput

et al. 2024

Front. Microbiol.

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Fusarium oxysporum f. sp. capsici (Foc) poses a significant position in agriculture that has a negative impact on chili plant in terms of growth, fruit quality, and yield. Biological control is one of the promising strategies to control this pathogen in crops. Chili is considered as one of the most important crops in the Hyderabad region that is affected by Fusarium wilt disease. The pathogen was isolated from the infected samples in the region and was confirmed by morphological characteristics and PCR with a band of 488 bp. The bacterial strains were isolated from the rhizosphere soil of healthy plant and also confirmed by PCR with a band of 1,542 bp.The molecular characterization of the fungal and bacterial strain has shown 99.9% homology with the retrieved sequences of Fusarium oxysporum f. sp. capsici and Bacillus subtilis from NCBI. The 1-month-old Ghotki chili plants were inoculated with 1×105 cfu spore/ml−1 suspension and confirmed that the FOC-1 is responsible for chili Fusarium wilt disease. Subsequently, among the 33 screened Bacillus strains, only 11 showed antagonistic activity against F. oxysporum. Out of these, only two strains (AM13 and AM21) have shown maximum antagonistic activity against the pathogen by reducing the infection and promoting growth parameters of chili plants under both in vitro and greenhouse conditions. The study suggested that biological control is the most promising control strategy for the management of Fusarium wilt of chili in the field.

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“…The fermentation for bio‐surfactants production was done in a static batch culture of TGE medium (comprising glucose‐1%, tryptone‐1%, yeast‐1%, magnesium sulfate‐0.005%, manganese sulfate‐0.005%, pH 6.8 ± 2) at 37°C for 48 h. After incubation, the culture aliquot was harvested by centrifugation at 3174 × g for 10 min at 4°C. After that 50% methanol was added to the supernatant followed by vigorous shaking for 10 min (Kumar et al, 2021; Kumari et al, 2012). The culture aliquot was then concentrated in a rotary vacuum evaporator (Superfit R‐150, Mumbai, India).…”

Section: Methodsmentioning

confidence: 99%

Antibiofilm and antimicrobial activity of biosurfactants from two Lactiplantibacillus pentosus strains against food and topical pathogens

Parvin

Adhikary

Guha

et al. 2022

Food Processing Preservation

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The present study aims to evaluate the antimicrobial and antibiofilm properties of the biosurfactants from Lactiplantibacillus pentosus MSCIN-24 (GenBank Ac. No.: MK397507) and L. pentosus No.: MK397508) against food spoilage and topical pathogens. The extracellular biosurfactants were produced in static broth culture at pH 6.8 ± 2, 37°C for 48 h and harvested by ethanol precipitation. The biosurfactants from these strains had the highest E 24 values of 100% and 93.75% in olive oil, respectively at a minimum dose of 50 mg/ml and were thermostable and enzyme stable. These glycolipid biosurfactants had broad-spectrum antimicrobial activity against the food and topical pathogens with MIC and antibiofilm values in the range of 5 to 15 mg/ml and caused cell disruption. Thus, these broadspectrum antibiofilm glycolipid biosurfactants could be used as potent antimicrobials against biofilm-forming food spoilage and topical pathogens. Practical applicationsBiofilm formation by food spoilage and topical pathogens are an important concern in the food processing industries for the health and hygiene issues of processed foods.Many commercial synthetic food preservatives show ineffective to combat these microorganisms. The biosurfactants from the food-grade Lactiplantibacillus pentosus MSCIN-24 and MSCIN-25 show promising antimicrobial and antibiofilm properties against the food spoilage and topical pathogens that could be exploited in food processing and preservation to extend their shelf life and better hygiene.

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Antagonistic activity of lipopeptide-biosurfactant producing Bacillus subtilis AKP, against Colletotrichum capsici, the causal organism of anthracnose disease of chilli

Cited by 21 publications

References 50 publications

Biosurfactant Activity of Bacillus sp. Strain LP04 Isolate and Its Antifungal Potency against Ganoderma boninense and Fusarium sp.

Biosurfactant Activity of Bacillus sp. Strain LP04 Isolate and Its Antifungal Potency against Ganoderma boninense and Fusarium sp.

Antagonistic activity of two Bacillus strains against Fusarium oxysporum f. sp. capsici (FOC-1) causing Fusarium wilt and growth promotion activity of chili plant

Antibiofilm and antimicrobial activity of biosurfactants from two Lactiplantibacillus pentosus strains against food and topical pathogens

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