Antagonistic Bacillus cereus TC-1 Isolated from Solar Salt Work in Southern India

Donio, M B S; Viji, V.; Jenifer, J Adlin; Velmurugan, Subramanian; Raman, K.; Babu, Mariavincent Michael; Citarasu, Thavasimuthu

doi:10.3329/bjm.v29i1.28431

Cited by 2 publications

(2 citation statements)

References 23 publications

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“…Many species within this genus have been proven to be safe, even as fermentation strains of food and as probiotics drugs for oral consumption (Bacon and Hinton 2002). Some reports stated that Bacillus could produce biological control agents and has become one of the most important sources of probiotics (Ravi et al 2007;Barman et al 2011;Zokaeifar et al 2012;Donio et al 2014). However, the application of Bacillus spp.…”

Section: Introductionmentioning

confidence: 96%

Isolation and characterisation of Bacillus spp. antagonistic to Vibrio parahaemolyticus for use as probiotics in aquaculture

Liu

Wang

Lǐ

et al. 2015

World J Microbiol Biotechnol

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Acute gastroenteritis caused by pathogenic Vibrio parahaemolyticus is one of the major factors affecting the development of aquaculture and the safety of seafood. Using the antagonism of probiotics against pathogens is an alternative strategy to antibiotics and a common trend to control food-borne pathogenic bacteria. In this study, a total of 249 isolates were isolated from four types of seafood (Litopenaeus vannamei, Oratosquilla oratoria, Mactra veneriformis and Portunus trituberculatus) and coastal sediment from Liaodong Bay in the Bohai Sea, China with five different separation agars. The most isolates came from the sample of coastal sediment and on agar of 2216E, which accounted for 36.14 and 54.62 % respectively. Twenty-four among 249 isolates displayed direct antimicrobial activity to V. parahaemolyticus with spot inoculation. Sixteen active isolates were selected for extracellular antimicrobial activity using the Oxford cup method. Only strains of B16 and J7 showed extracellular antimicrobial activity and were identified as Bacillus pumilus and Bacillus mojavensis respectively based on the physiological identification and 16S rRNA sequence analysis. Both of the strains B16 and J7 exhibited extracellular hydrolytic enzyme activity and antagonism against more than one indicator bacteria in vitro, which indicates that the two strains have broad potential application as suitable probiotic candidates in aquaculture while B. mojavensis was first reported to inhibit pathogenic Vibrio spp. in vitro. There is no particular trait as to antagonism of B. pumilus B16 or B. mojavensis J7 to Gram-positive or Gram-negative indicator bacteria.

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Section: Introductionmentioning

confidence: 96%

Isolation and characterisation of Bacillus spp. antagonistic to Vibrio parahaemolyticus for use as probiotics in aquaculture

Liu

Wang

Lǐ

et al. 2015

World J Microbiol Biotechnol

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“…Occasionally, Bacillus spp. are important microbe which not only act as probiotic but also produce biological control agent (Donio et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Characterization of potential probionts from blue swimming crab Portunus pelagicus and its antagonistic activity against Vibrio harveyi

Rahman¹,

Esa²,

Yasin³

et al. 2017

Int. J. Biosci.

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The aim of this study was to isolate local bacteria as potential probiont in controlling the growth of pathogenic Vibrio harveyi. Eleven potential bacteria were successfully isolated from the haemolymph of four healthy Portunus pelagicus. All of the isolates were identified as Bacillus amyloliquefaciens by series of biochemical test using triple sugar ion test, oxidase and catalase test, followed by Internal Transcribe Spacer (ITS) gene sequence analysis. This isolate was able to inhibit the growth of V. harveyi in in-vitro screening assay by using well diffusion assay with the strong antagonistic activity from 7 to 16 mm. The potential strains at 10 8 CFU/ml showed highest inhibition response towards V. harveyi after co-cultured for 48 hr. The isolates produced four major extracellular enzymes which were amylase, protease, gelatinase and lipase and able to form biofilm after 24 hr of cultured. Thus, B. amyloliquefaciens showed important characteristics as probiotic which worth to be carried out for in vivo challenge assay.

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Antagonistic Bacillus cereus TC-1 Isolated from Solar Salt Work in Southern India

Cited by 2 publications

References 23 publications

Isolation and characterisation of Bacillus spp. antagonistic to Vibrio parahaemolyticus for use as probiotics in aquaculture

Isolation and characterisation of Bacillus spp. antagonistic to Vibrio parahaemolyticus for use as probiotics in aquaculture

Characterization of potential probionts from blue swimming crab Portunus pelagicus and its antagonistic activity against Vibrio harveyi

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