Antagonistic Effect of Two Indigenous Phosphate Solubilizing Bacteria, Burkholderia contaminans PSB3 and Acinetobacter baumannii PSB11 Isolated from Different Crop Soils

Abstract

Phosphorus is the most important key element in the nutrition of plants. Although P is abundant in soils, it is a major limiting factor for plant growth as it is in an unavailable form for roots uptake. Phosphate solubilizing bacteria (PSB) has ability to convert insoluble form of P to an available form. This study was aimed at screening and characterizing phosphate-solubilizing bacteria from manure and different rhizosphere and to ascertain a potential benefit to use mixed cultures to improve P solubilization. A total of 12 PSB colonies were isolated on Pikovskaya’s agar medium containing tricalcium phosphate. Out of 12 bacterial isolates, 2 isolates showed high phosphate solubilization index (2.17 and 1.83, respectively) were selected for further study. Based on the 16S rRNA gene sequence analysis, PSB3 was closely related to Burkholderia contaminans (99%), and PSB11 was closely related to Acinetobacter baumannii (99%). The mean P dissolved in liquid cultures of PSB3 and PSB11 in a 14-day incubation were 96.7 and 39.3 mg l-1, respectively. Mixed inoculation of B. contaminans PSB3 and A. baumannii PSB11 could not increase the solubilization activity significantly, suggesting there is antagonistic behavior of one isolate towards another. As the interaction of these two isolates may be antagonistic, co-inoculation of these bacteria for P solubilization is not recommended. However, further study is needed to confirm these results.

Exploration and Characterization of High-Efficiency Phosphate-Solubilizing Bacteria Isolates from Chickpea Rhizospheric Soil

“…The FASTA sequence was deposited in GenBank under the accession number OK510279 and OK337612 (Figure 3) Molecular identification of selected isolates and potentially efficient PSB strains based on the sequencing of 16S rRNA and phylogenetic affiliation confirmed that the V 7 isolates and belonged to the genus Acinetobacter and V 8 isolate Penibacillus respectively (Figure 4). The phylogenetic tree revealed that the isolate V 8 (VK1) been obtained from various soils and found to have inorganic phosphate (IP) solubilization such as, Acinetobacter calcoaceticus Goosen et al (1989) and Acinetobacter baumannii (Nugroho et al, 2020). In some Paenibacillus species such as Paenibacillus mucilaginosus (Hu et al, 2006), Paenibacillus elgii (Narayan et al, 2010), Paenibacillus kribbensis (Marra et al, 2012), Paenibacillus polymyxa, Paenibacillus macerans (Wang et al, 2012), and Paenibacillus xylanilyticus (Pandya et al, 2015), the Phosphate Solubilization ability has been confirmed.…”

Improving yield of Barley using bio and nano fertilizers under saline conditions

“…Brevundimonasolei (MJ15) identified by (Omar and Ibrahim, under press). Nugroho et al (2020) studied established that the Phosphate solubilizing bacteria (PSB) has ability to convert insoluble form of P to an available form, the mean P dissolved in liquid culture of A. baumanniiPSB11 after 14 day incubation was (39.3 mg l -1 ), andstrain SWSI1725 of the genus Bacillus showed the strongest ability with a phosphate-solubilizing content of (103.57 mg l -1 ), respectively.…”

Isolation and identification of Cr-resistant bacteria from the rhizosphere of Tagetes sp. and their ability to reduce Cr(VI)

“…The bacterial isolates on 48-hour-old slanted LB agar were extracted their genomic DNA using the Quick-DNA Fungal/Bacterial Miniprep Kit (Zymo Research, D6005). The 16S rRNA gene was amplified using PCR and universal primers of 27F (5'-AGAGTTTGATCMTGGCTCAG-3') and 1492R (5′-TACCTTGTTACGACTT-3') (Nugroho et al 2020). All reactions were carried out in a 25 uL reaction mixture consisting of 12.5 L of MyTaq HS Red Mix, 2x (Bioline, BIO-25046), 1 L of each primer (10 M), 1 L of BSA (Biolabs, 10 mg mL -1 ), 1 L of DNA genome, and 8.5 L ddH2O (MP Biomedicals).…”

“…The amplification was done using a thermal cycler (Eppendorf Nexus GSX1). PCR was carried out with conditions of initial denaturation step at 94C for 5 min, followed by 35 cycles of denaturation step at 94C for 30 s, primer annealing at 54C for 30 s, and elongation at 72C for 30 s (Nugroho et al 2020). The reaction was completed with an extension step at 72C for 10 min.…”

“…SpR3 may occur, resulting in a lower rate of Cr(VI) reduction. In mixed cultures, antagonism (Nugroho et al 2020) or synergism (Dutta et al 2021) effects can occur, so the use of mixed cultures needs further study before being applied in the environmental bioremediation process.…”