Antagonistic effects in zebrafish (Danio rerio) behavior and oxidative stress induced by toxic metals and deltamethrin acute exposure

Jijie, Roxana; Solcan, G.; Nicoară, Mircea; Micu, Dragos; Strungaru, Ștefan-Adrian

doi:10.1016/j.scitotenv.2019.134299

Cited by 65 publications

(32 citation statements)

References 59 publications

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“…C supplements to reduce the harmful effects of the insecticides. SOD and GPx are considered to be the first line of defense against oxidative stress since SOD helps in conversion of O 2 − to O 2 and H 2 O 2 while GPx can effectively catalyze the reduction of H 2 O 2 to water and a variety of organic peroxides to alcohols [ 52 ]. As can be seen in Figure 4 , all three biochemical indicators of the oxidative stress resulted in a significant variance ( p < 0.05 ANOVA).…”

Section: Resultsmentioning

confidence: 99%

“…C supplementation in water reduced the mortality, the genotoxicity, and biochemical damage resulted from fipronil and buprofezin exposure. Meanwhile, in the case of SOD and GPx, zebrafish were found to adopt a mechanism to compensate for the oxidative damage induced by deltamethrin, another insecticide that is also neurotoxic, [ 52 , 74 ] resulting in an increment of their values. This mechanism is active in the embryonic stage of development for zebrafish [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

“…Later, the results obtained after this test proved that all of the data sets of the experimental groups were normally distributed. Afterwards, the one-way ANOVA followed by the Tukey HSD (honestly significant difference) test were performed to demonstrate the significant different variance of the investigated behavioral variables from the initial condition of the subjects (initial behavior) to the end of the treatment in the case of each group [ 52 , 55 ]. In the case of oxidative stress, the variance was calculated with multiple comparisons between control and treatment groups.…”

Section: Methodsmentioning

confidence: 99%

“…Danio rerio (Hamilton, 1822), commonly known as zebrafish, are widely used as vertebrate models for prediction of pollutants (neuro)toxicity in aquatic systems due to their complex behavioral responses, short breeding cycle, transparent embryos, and high similarity with human genome [ 51 , 52 ]. Taken together, the aim of this study was to evaluate the possible protective role of vit.…”

Section: Introductionmentioning

confidence: 99%

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Vitamin C Attenuates Oxidative Stress and Behavioral Abnormalities Triggered by Fipronil and Pyriproxyfen Insecticide Chronic Exposure on Zebrafish Juvenile

et al. 2020

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Chronic exposure to synthetic insecticides in the early life of a child can lead to a series of disorders. Several causes as parental age, maternal smoking, birth complications, and exposure to toxins such as insecticides on childhood can lead to Autism spectrum disorder (ASD) occurrence. The aim of this study was to evaluate the potential protective role of vitamin C (Vit. C) from children’s supplements after 14 days chronic exposure to insecticide mixture fipronil (Fip) + pyriproxyfen (Pyr) on juvenile zebrafish for swimming performances, social behavior and oxidative stress associated with ASD model. Juvenile (14–17 mm) wild-type AB zebrafish (Danio rerio) (45 days) were exposed to relevant concentrations: vit. C (25 µg L−1), Fip (600 µg L−1/1.372 μM) + Pyr (600 µg L−1/1.89 μM), and [Fip (600 µg L−1/1.372 μM) + Pyr (600 µg L−1 /1.89 μM)] + vit. C (25 µg L−1). Our results showed that insecticides can disturb the social behavior of zebrafish during 14 days of the administration, decreased the swimming performances, and elevated the oxidative stress biomarkers of SOD (superoxide dismutase), GPx (glutathione peroxidase), and MDA (malondialdehyde). The vitamin C supplement significantly attenuated the neurotoxicity of insecticide mixture and oxidative stress. This study provides possible in vivo evidence to show that vitamin C supplements could attenuate oxidative stress and brain damage of fipronil and pyriproxyfen insecticide chronic exposure on zebrafish juvenile.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Vitamin C Attenuates Oxidative Stress and Behavioral Abnormalities Triggered by Fipronil and Pyriproxyfen Insecticide Chronic Exposure on Zebrafish Juvenile

et al. 2020

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“…It is a water-soluble product and was diluted for the experimental concentrations: 0.25, 0.5, 1, and 2 µg L −1 . We conducted before three experiments where it was demonstrated that at the studied concentrations there is no mortality and these were reported in various studies (between 0.04 and 24 µg L −1 in Canadian agricultural areas; 0.73-58.8 ng L −1 in the Ebro Delta, Spain) [36][37][38]. After each 24 h the water was replaced from each aquarium and the medium with deltamethrin was redone.…”

Section: Experimental Designmentioning

confidence: 99%

Toxicity of Deltamethrin to Zebrafish Gonads Revealed by Cellular Biomarkers

Petrovici

Strungaru

Nicoară

et al. 2020

JMSE

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Deltamethrin is responsible for health and reproduction problems both in mammals and aquatic organisms. In this study, zebrafish adults were exposed for 15 days to 0.25, 0.5, 1, and 2 μg L−1 non-lethal concentrations of deltamethrin, knowing that is used worldwide on agricultural crops. We investigated the chronic effects of deltamethrin on gonads by histopathological examination, immunohistochemistry, and immunofluorescence using biomarkers for apoptosis (anti-p53, anti-H2A.XS139ph antibodies, and TUNEL assay), oxidative stress (anti-Cox4i1 antibody) and proliferation (anti-PCNA antibody). Among the histopathological changes, the apoptotic response was elevated in ovary and testis of deltamethrin exposed groups as it was seen in the IHC and IF for p53, H2A.XS139ph, and confirmed by TUNEL assay. These were observed in the case of all studied concentrations compared with the control group. Thereby, the gonadal tissue exhibited an up-regulated activity of this cell-death signaling markers, while the proliferation marker (PCNA) increased in the ovary due to its presence not only in primary growth and cortical-alveolar stage follicles but also in atretic follicles, meanwhile decreased notably in the testis. Cox4i1, a mitochondrial marker, decreased both in ovary and testis during deltamethrin treatment, probably inhibited by the overproduction of the free radicals after pesticide exposure.

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Phenomics Approach to Investigate Behavioral Toxicity of Environmental or Occupational Toxicants in Adult Zebrafish (Danio rerio)

et al. 2021

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Over the last few years, environmental pollution, especially water pollution, has become a serious issue worldwide. Thus, methods that can help us understand the impact and effects of these pollutants, especially on aquatic animals, are needed. Behavioral assessment has emerged as a crucial tool in toxicology and pharmacology because many studies have shown, in multiple animal models, that various pharmacological compounds can alter behavior, with many of the findings being translatable to humans. Moreover, behavior study can also be used as a suitable indicator in the ecotoxicological risk assessment of pollutants. Several model organisms, especially rodent models, have been extensively employed for behavior studies. However, assessments using this model are generally time consuming, expensive, and require extensive facilities for housing experimental animals. Moreover, behavioral studies typically use different measurements and assessment tools, making comparisons difficult. In addition, even though behavioral phenomics has the potential to comprehensively illustrate the toxicities of chemicals, there is only a limited number of studies focusing on animal behavior using such a global approach. Here, we describe a phenomics approach that can be used to investigate the impact of pollutants using zebrafish. The approach consists of several behavioral tests, including response to a novel environment, mirror‐reflection image, predator fish, and conspecifics, after exposure to a test chemical. Phenotype fingerprinting, a method for summarizing individual phenotypes based on the results of the behavioral tests, is then conducted to reduce data complexity and display the pattern of each compound on behavioral phenotypes in zebrafish. This approach may be useful to researchers studying the potential adverse effects of different pollutants. © 2021 Wiley Periodicals LLC. Basic Protocol 1: Novel tank test Basic Protocol 2: Shoaling test Basic Protocol 3: Aggression test (mirror biting test) Basic Protocol 4: Social interaction test Basic Protocol 5: Fear response test Basic Protocol 6: PCA and heatmap clustering

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Antagonistic effects in zebrafish (Danio rerio) behavior and oxidative stress induced by toxic metals and deltamethrin acute exposure

Cited by 65 publications

References 59 publications

Vitamin C Attenuates Oxidative Stress and Behavioral Abnormalities Triggered by Fipronil and Pyriproxyfen Insecticide Chronic Exposure on Zebrafish Juvenile

Vitamin C Attenuates Oxidative Stress and Behavioral Abnormalities Triggered by Fipronil and Pyriproxyfen Insecticide Chronic Exposure on Zebrafish Juvenile

Toxicity of Deltamethrin to Zebrafish Gonads Revealed by Cellular Biomarkers

Phenomics Approach to Investigate Behavioral Toxicity of Environmental or Occupational Toxicants in Adult Zebrafish (Danio rerio)

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