Antagonistic Impact of Acrylamide and Ethanol on Biochemical and Morphological Parameters Consistent with Bone Health in Mice

Abstract

The aim of present study was to verify antagonistic effect of acrylamide (AA) and ethanol (Et) on bone quality parameters. Adult mice (n = 20) were segregated into four groups following 2 weeks administration of toxins: group E1, which received AA (20 mg/kg body weight daily); group E2, which received 15% Et (1.7 g 100% Et/kg body weight daily); group E12, which received simultaneously both toxins; and a control group. An insignificant impact of individual applications of AA, Et or their simultaneous supplementation on the total body weight of mice and the length and weight of their femoral bones was identified. In group E1, higher levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), a decreased level of glutathione (GSH) and elevated endocortical bone remodelling were determined. A significantly lower relative volume of cortical bone, bone mineral density (BMD), elevated endocortical bone remodelling and cortical porosity, higher levels of ALT, AST, lower values for total proteins (TP), GSH, alkaline phosphatase (ALP), calcium, and phosphorus were recorded in group E2. In the mice from group E12, the highest endocortical bone remodelling, decreased values for BMD, TP, GSH and ALP and increased levels of ALT and AST were found. Our findings confirmed the antagonistic impact of AA and Et at doses used in this study on biochemical and morphological parameters consistent with bone health in an animal model.

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“…Our findings from histological analysis of the cortical bone in both W and C groups are consistent with other researches, which on the one hand indicate the possibility of intracortical bone remodeling, but on the other hand confirm the predominance of parallel lamellae over concentric ones in rodents [ 22 , 53 , 54 , 55 , 56 ]. Several SO we identified lacked numerous concentric lamellae and did not show the highly organized networks observed in humans [ 54 ].…”

“…For histological analysis of the cortical bone, femurs were cut at the diaphyseal midshaft. Transverse thin sections (70–80 μm) were prepared according to Martiniakova et al [ 22 ] and were visualized under light microscope with polarization (Leica DM 2000, Germany). The quantitative 2D parameters were assessed using Motic Images Plus 2.0 ML (Motic China Group Co., Ltd., Nanjing, China) and Image J [ 23 ].…”

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“…The result is a number of common publication outputs in databases: [50,51]. In 2020, significant international cooperation was also developed with scientists from Turkey, Bulgaria, and Poland [52][53][54]…”