Antagonistic potential of Trichoderma as a biocontrol agent against Sclerotinia asari

Abstract

In the present study, the inhibitory potential of 14 Trichoderma strains (isolated from Asarum rhizosphere) was investigated against Sclerotinia asari using the plate dilution method. The activity of antioxidant enzymes viz; catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), and malondialdehyde (MDA) in S. asari treated with the two Trichoderma strains was also evaluated. Untargeted metabolomic analysis by using LC/MS analysis was carried out to determine differential metabolites in T. hamatum (A26) and T. koningiopsis (B30) groups. Moreover, transcriptome analysis of S. asari during the inhibition of S. asari by B30, and A26 compared with the control (CK) was performed. Results indicated that inhibition rates of T. koningiopsis B30, and T. hamatum A26 were highest compared to other strains. Similarly, non-volatile metabolites extracted from the B30 strain showed a 100% inhibition of S. asari. The activity of CAT, SOD, and POD decreased after treatment with A26 and B30 strains while increasing MDA content of S. asari. Antifungal activity of differential metabolites like abamectin, eplerenone, behenic acid, lauric acid, josamycin, erythromycin, and minocycline exhibited the highest inhibition of S. asari. Transcriptome analysis showed that differentially expressed genes were involved in many metabolic pathways which subsequently contributed toward antifungal activity of Trichoderma. These findings suggested that both Trichoderma strains (B30 and A26) could be effectively used as biocontrol agents against Sclerotinia disease of Asarum.

The effect of Trichoderma harzianum agents on physiological-biochemical characteristics of cucumber and the control effect against Fusarium wilt

“…At the same time, 30 days after cucumber sowing, the MDA level in the leaves of T3 cucumber seedlings was 8.40% greater than that of CK2, and it was 47.68% lower than that of CK1. This could be because T. harzianum , which interacts with cucumber, induces protective enzymes such as CAT, POD, SOD, APX, and PPO in cucumber plants under pathogen infection, removes reactive oxygen species (ROS) in plants 60 , reduces MDA content 61 , and reduces cell membrane damage caused by membrane lipid peroxidation under disease stress.…”

Microfluidic platform for microbial spore germination studies in multiple growth conditions

“…After demonstrating the use of the 4CMC device for both model and non-model soil bacteria, we chose to explore its usability to study soil fungi. There has been a lot of interest in recent years in using Trichoderma species as biocontrol agents (Huma 2022;Li et al 2022;Manzar et al 2022;Wang et al 2022;Risoli et al 2023;Sorathiya et al 2023), making it particularly important to understand how Trichoderma spores respond to their environment. Here, the responsiveness to nutrient availability was investigated using T. rossicum.…”

Trichoderma hamatum and Its Benefits

“…Further, it is proved that non-volatile compounds, such as Abamectin, Eplerenone, Bhenic acid, Josamycin, Erythromycin, Methyleugenol, and Minocycline, evinced significant antifungal activity. Hence, T. koningiopsis and T. hamatum suggested as biocontrol agents for the treatment of Asarum sclerotiorum [61]. However, Trichoderma spp.…”