Antagonistic Strain Bacillus amyloliquefaciens XZ34-1 for Controlling Bipolaris sorokiniana and Promoting Growth in Wheat

Yi, Yanjie; Shan, Youtian; Liu, Shifei; Yang, Yanhui; Liu, Yang; Yin, Yanan; Hou, Zhipeng; Luan, Pengyu; Li, Ruifang

doi:10.3390/pathogens10111526

Cited by 25 publications

(37 citation statements)

References 62 publications

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“…Biological control has been recognized as a promising and sustainable measure because of less pollution to the environment and a lower health danger compared with the chemical control. B. amyloliquefaciens with a higher antagonistic activity against phytopathogens can be successfully used as a potential biocontrol agent to control diverse crop diseases, including potato scab caused by Streptomyces griseoplanus [ 24 ]; wheat root rot caused by Bipolaris sorokiniana [ 25 ]; ginseng gray mold caused by B. cinerea [ 26 ]; alfalfa anthracnose caused by C. truncatum [ 27 ]; tomato bacterial wilt caused by Ralstonia solanacearum [ 28 ]; tomato bacterial canker caused by Clavibacter michiganensis [ 29 ]; wheat Fusarium head blight caused by F. graminearum [ 30 ]; and sugar beet leaf spot disease caused by Pseudomonas syringae pv. aptata [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

Biological Control of Verticillium Wilt and Growth Promotion in Tomato by Rhizospheric Soil-Derived Bacillus amyloliquefaciens Oj-2.16

et al. 2022

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Verticillium wilt disease caused by Verticillium dahliae seriously affects tomato quality and yield. In this work, strain Oj-2.16 was isolated from rhizosphere soil of the medicinal plant Ophiopogon japonicas and identified as Bacillus amyloliquefaciens on the basis of morphological, physiological, and biochemical characteristics and 16S rDNA sequencing. Strain Oj-2.16 exhibited a high inhibition rate against V. dahliae, and the hyphae inhibited by Oj-2.16 were found to be destroyed on scanning electron microscopy. Lipopeptide and dipeptide genes were detected in the Oj-2.16 genome by PCR amplification involved in surfactin, iturin, fengycin, and bacilysin biosynthesis. In pot experiments, the biocontrol efficacy of strain Oj-2.16 against Verticillium wilt in tomato was 89.26%, which was slightly higher than the efficacy of the chemical fungicide carbendazim. Strain Oj-2.16 can produce indole acetic acid, siderophores, assimilate various carbon sources, and significantly promoted the growth of tomato seedlings by increasing plant height, root length, stem width, fresh weight, and dry weight by 44.44%, 122.22%, 80.19%, 57.65%, 64.00%, respectively. Furthermore, defense-related antioxidant CAT, SOD, POD, and PAL enzyme activities significantly increased and MDA contents significantly decreased in tomato seedlings treated with strain Oj-2.16 upon inoculation of V. dahliae compared with the pathogen-inoculated control. In summary, we concluded that B. amyloliquefaciens Oj-2.16 could be used as a promising candidate for the biocontrol of Verticillium wilt and as plant growth stimulator of tomato.

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Section: Discussionmentioning

confidence: 99%

Biological Control of Verticillium Wilt and Growth Promotion in Tomato by Rhizospheric Soil-Derived Bacillus amyloliquefaciens Oj-2.16

et al. 2022

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“…B. velezensis ZF2 has been reported to produce protease (Prt) and cellulase (Cel), which may inhibit the mycelial growth of C. cassiicola and suppress cucumber leaf spot disease [9]. The current study demonstrated that B. amyloliquefaciens strain XZ34-1, as a biocontrol agent for the control of Bipolaris sorokiniana, has the ability to produce protease and pectinase [36]. In our study, GSBZ09 produced cellulase, protease, amylase and lipase, which may have resulted in the observed morphological changes and growth inhibition of C. vitis hyphae.…”

Section: Discussionmentioning

confidence: 69%

Suppression of Grape White Rot Caused by Coniella vitis Using the Potential Biocontrol Agent Bacillus velezensis GSBZ09

Yin

Jiang

et al. 2022

Pathogens

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Grape white rot caused by Coniella vitis is prevalent in almost all grapevines worldwide and results in a yield loss of 10–20% annually. Bacillus velezensis is a reputable plant growth-promoting bacterial. Strain GSBZ09 was isolated from grapevine cv. Red Globe (Vitis vinifera) and identified as B. velezensis according to morphological, physiological, biochemical characteristics and a multilocus gene sequence analysis (MLSA) based on six housekeeping genes (16S rRNA, gyrB, rpoD, atpD, rho and pgk). B. velezensis GSBZ09 was screened for antifungal activity against C. vitis under in vitro and in vivo conditions. GSBZ09 presented broad spectrum antifungal activity and produced many extracellular enzymes that remarkably inhibited the mycelial growth and spore germination of C. vitis. Furthermore, GSBZ09 had a high capacity for indole-3-acetic acid (IAA) production, siderophore production, and mineral phosphate solubilization. Pot experiments showed that the application of GSBZ09 significantly decreased the disease index of the grape white rot, directly promoted the growth of grapes, and upregulated defense-related enzymes. Overall, the features of B. velezensis GSBZ09 make it a potential strain for application as a biological control agent against C. vitis.

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“…Several lines of evidence suggest that acetoin is a powerful elicitor to trigger induced systemic resistance in plants [66]. Yi et al [67] demonstrated that volatile acetoin produced by B. amyloliquefaciens UCMB5113 significantly reduces infection of Bipolaris sorokiniana and promotes the growth of wheat seedlings compared with seedlings not exposed to bacterial volatiles before pathogen inoculation. The presence of gene clusters for the biosynthesis of acetoin in all of our Bacilli suggested that they induce systemic resistance in wheat plants and suppress wheat blast disease.…”

Section: Discussionmentioning

confidence: 99%

Probiotic <em>Bacillus</em> Species: Promising Biological Control Agents for Managing Worrisome Wheat Blast Disease

Surovy¹,

Dutta²,

Mahmud³

et al. 2022

Preprint

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Plant diseases are among the major factors affecting plant productivity. Biological control of plant diseases is preferred over chemical control as it is environment-friendly, cost-effective, and sustainable. Among many microbes capable of providing biological control of plant diseases, probiotic Bacillus species are most promising as they can survive in adverse conditions, provide plants with a wide range of benefits including protection from phytopathogens. Wheat blast caused by Magnaporthe oryzae Triticum pathotype (MoT) has emerged as a potential threat to global wheat production. Due to unreliability of fungicides and limited cultivar resistance, we aimed to screen and identify potential antagonist bacteria collected from internal tissues of rice and wheat seeds to determine their in vitro and in vivo inhibitory effects against MoT. Dual culture and seedling assays were performed to evaluate the efficacy of probiotic bacteria. Out of 170 bacterial isolates, three bacteria (BTS-3, BTS-4, and BTLK6A) were screened as potential antagonists against MoT in vitro. Artificial inoculation at the seedling stage showed that the isolates BTS-4, BTS–3, and BTLK6A reduced 89, 88, and 85% of wheat blast disease severity, respectively, compared to mock-inoculated control. The bacterial isolates were identified as Bacillus subtilis (BTS-3) and B. velezensis (BTS-4 and BTLK6A) through genome phylogeny. The whole genome sequence of these three bacterial strains decoded a number of orthologs to intrinsic genes of antimicrobial peptides, antioxidant defense enzymes, cell wall degrading enzymes, compounds involved in the induction of systemic resistance (ISR) in host plants, and volatile compounds to make them promising biologicals to control MoT in wheat. Combined data of in vitro and in vivo along with genome analysis suggest that Bacillus spp. suppress the destructive wheat blast disease likely through antibiosis and ISR in the host plants. Further field evaluation and characterization of antimicrobial compounds are needed for a better understanding of the mode of action and practical recommendation of these bacteria for wheat blast control in the farmers’ fields.

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Antagonistic Strain Bacillus amyloliquefaciens XZ34-1 for Controlling Bipolaris sorokiniana and Promoting Growth in Wheat

Cited by 25 publications

References 62 publications

Biological Control of Verticillium Wilt and Growth Promotion in Tomato by Rhizospheric Soil-Derived Bacillus amyloliquefaciens Oj-2.16

Biological Control of Verticillium Wilt and Growth Promotion in Tomato by Rhizospheric Soil-Derived Bacillus amyloliquefaciens Oj-2.16

Suppression of Grape White Rot Caused by Coniella vitis Using the Potential Biocontrol Agent Bacillus velezensis GSBZ09

Probiotic <em>Bacillus</em> Species: Promising Biological Control Agents for Managing Worrisome Wheat Blast Disease

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