Antarctic bacterial isolates that produce cold-active extracellular proteases at low temperature but are active and stable at high temperature

MartÃ­nez-Rosales, Cecilia; Castro‐Sowinski, Susana

doi:10.3402/polar.v30i0.7123

Cited by 37 publications

(14 citation statements)

References 26 publications

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“…orientalis (group I, 16 strains) has been isolated from spring waters in Lebanon [ 30 ]; P . simiae (group III, 7 strains) has been isolated from a monkey [ 31 ] and from Antarctic samples [ 32 ]; P . lurida (group IV, 5 strains) has been found as a plant growth promoting bacterium in the phyllosphere of grasses [ 33 ] and in high altitude rhizospheric soil from the Uttarakh and Himalayas [ 34 , 35 ]; P .…”

Section: Discussionmentioning

confidence: 99%

New Pseudomonas spp. Are Pathogenic to Citrus

et al. 2016

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Five putative novel Pseudomonas species shown to be pathogenic to citrus have been characterized in a screening of 126 Pseudomonas strains isolated from diseased citrus leaves and stems in northern Iran. The 126 strains were studied using a polyphasic approach that included phenotypic characterizations and phylogenetic multilocus sequence analysis. The pathogenicity of these strains against 3 cultivars of citrus is demonstrated in greenhouse and field studies. The strains were initially grouped phenotypically and by their partial rpoD gene sequences into 11 coherent groups in the Pseudomonas fluorescens phylogenetic lineage. Fifty-three strains that are representatives of the 11 groups were selected and analyzed by partial sequencing of their 16S rRNA and gyrB genes. The individual and concatenated partial sequences of the three genes were used to construct the corresponding phylogenetic trees. The majority of the strains were identified at the species level: P. lurida (5 strains), P. monteilii (2 strains), P. moraviensis (1 strain), P. orientalis (16 strains), P. simiae (7 strains), P. syringae (46 strains, distributed phylogenetically in at least 5 pathovars), and P. viridiflava (2 strains). This is the first report of pathogenicity on citrus of P. orientalis, P. simiae, P. lurida, P. moraviensis and P. monteilii strains. The remaining 47 strains that could not be identified at the species level are considered representatives of at least 5 putative novel Pseudomonas species that are not yet described.

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Section: Discussionmentioning

confidence: 99%

New Pseudomonas spp. Are Pathogenic to Citrus

et al. 2016

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“…Relevant biotechnological cold‐active bacterial enzymes have been identified using culture‐dependent studies (Margesin & Schinner, ; Vazquez et al ., ; Martínez‐Rosales & Castro‐Sowinski, ; among many others). Currently, however, the most promising approach is based upon metagenomics, a culture‐independent genomic analysis.…”

Section: The Huge Potential Of Antarctic Bacterial Dnamentioning

confidence: 96%

Antarctic DNA moving forward: genomic plasticity and biotechnological potential

Martínez-Rosales

Fullana

Musto

et al. 2012

FEMS Microbiol Lett

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Antarctica is the coldest, driest, and windiest continent, where only cold-adapted organisms survive. It has been frequently cited as a pristine place, but it has a highly diverse microbial community that is continually seeded by nonindigenous microorganisms. In addition to the intromission of 'alien' microorganisms, global warming strongly affects microbial Antarctic communities, changing the genes (qualitatively and quantitatively) potentially available for horizontal gene transfer. Several mobile genetic elements have been described in Antarctic bacteria (including plasmids, transposons, integrons, and genomic islands), and the data support that they are actively involved in bacterial evolution in the Antarctic environment. In addition, this environment is a genomic source for the identification of novel molecules, and many investigators have used culture-dependent and culture-independent approaches to identify cold-adapted proteins. Some of them are described in this review. We also describe studies for the design of new recombinant technologies for the production of 'difficult' proteins.

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“…A solution of autoclaved skim milk % (w/v) was added in 1:1 (v/v) ratio to TSA diluted medium 1: 100 to achieve a final concentration of 5 % of skim milk. Protease activity was detected as a clear halo around the colony (36).…”

Section: Determination Of Siderophore Production and Xylanase β-Glucosidase Cellulase And Protease Activitiesmentioning

confidence: 99%

Paenibacillussp. strain UY79, isolated from a root nodule ofArachis villosa, displays a broad spectrum of antifungal activity

Costa¹,

Corallo

Amarelle³

et al. 2021

Preprint

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A nodule-inhabiting Paenibacillus sp. strain (UY79) isolated from wild peanut (Arachis villosa) was screened for its antagonistic activity against diverse fungi and oomycetes (Botrytis cinerea, Fusarium verticillioides, Fusarium oxysporum, Fusarium graminearum, Fusarium semitectum, Macrophomina phaseolina, Phomopsis longicolla, Pythium ultimum, Phytophthora sojae, Rhizoctonia solani, Sclerotium rolfsii and Trichoderma atroviride). Results obtained show that Paenibacillus sp. UY79 was able to antagonize these fungi/oomycetes and that agar-diffusible metabolites and enzymes, as well as volatile compounds (different from HCN), participate in the antagonism exerted. We found that Paenibacillus sp. strain UY79 did not affect symbiotic association or growth promotion of alfalfa plants when co-inoculated with rhizobia. By whole genome sequence analysis, we determined that strain UY79 is a new species of Paenibacillus within the Paenibacilluspolymyxa complex. Diverse genes putatively involved in biocontrol activity were identified in the UY79 genome. Moreover, according to genome mining and antibiosis assays, strain UY79 would have the capability to modulate the growth of bacteria commonly found in soil/plant communities.

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Antarctic bacterial isolates that produce cold-active extracellular proteases at low temperature but are active and stable at high temperature

Cited by 37 publications

References 26 publications

New Pseudomonas spp. Are Pathogenic to Citrus

New Pseudomonas spp. Are Pathogenic to Citrus

Antarctic DNA moving forward: genomic plasticity and biotechnological potential

Paenibacillussp. strain UY79, isolated from a root nodule ofArachis villosa, displays a broad spectrum of antifungal activity

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