Antarctic fungus proteases generate bioactive peptides from caseinate

Nascimento, Talita Camila Evaristo da Silva; Molino, João Vitor Dutra; Santos-Donado, Priscila Robertina dos; Montalvo, Gualberto Segundo Agamez; Santos, Wellington Leal dos; Gomes, José Erick Galindo; Santos, João H. P. M.; Silva, Roberto da; Sette, Lara Durães; Pessoa, Adalberto; Moreira, Keila Aparecida

doi:10.1016/j.foodres.2020.109944

Cited by 11 publications

(7 citation statements)

References 48 publications

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“…This is based on the fact that metalloproteases could constitute a part of the proteases secreted by Bacillus sp. bacteria and the cation-chelating ability of casein . Another explanation for the observed inhibitory effect is competitive inhibition.…”

Section: Results and Discussionmentioning

confidence: 99%

Multifunctional Casein-Based Wound Dressing Capable of Monitoring and Moderating the Proteolytic Activity of Chronic Wounds

Kolahreez,

Ghasemi-Mobarakeh,

Quartinello

et al. 2024

Biomacromolecules

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Every 1.2 s, a diabetic foot ulcer is developed, and every 20 s, one amputation is carried out in diabetic patients. Monitoring and controlling protease activity have been considered as a strategy for more efficient management of diabetic and other chronic wounds. This study aimed to develop a casein-based dressing that, by its disappearance, provides information about the activity of proteases and simultaneously harnesses proteolytic activity. Casein films were fabricated by using an aqueous solution, and heat treatment was successfully deployed as a green and clean approach to confer hydrolytic stability. Our results showed that casein-based films' mechanical characteristics, water absorption, and proteolytic stability could be controlled by the length of the heat treatment, which proved to be a useful tool. An increase in the treatment duration from 30 min to 3 h led to toleration of 2.4 times higher stress, 2 times lower water uptake, and 3.4 times higher proteolytic stability at examined conditions. Selected casein-based structures responded to Bacillus sp. bacteria's protease (BSP) and human neutrophil elastase (HNE) as representatives of bacterial and nonbacterial proteases found in the wounds at 10 and 200 ng mL −1 levels, respectively. The hydrolysis was accompanied by a 36% reduction in proteolytic activity measured by using a caseinbased universal protease activity assay. The released casein fragments could scavenge 90% of the examined radicals. In-vitro cell culture studies showed that the hydrolysates were not cytotoxic, and the casein-based film had a favorable interaction with fibroblast cells, indicating its potential as a scaffold in the case that proteolytic activity would not be to the extent that causes its rapid disintegration. In general, these findings hold promise for applying the developed casein-based structure for detecting proteolytic activity without the need for any equipment, kits, or expertise and, more importantly, in a highly economical manner. In the case that the proteolytic activity would not be severe, it could also serve as a substrate for cell adhesion and growth; this would aid in the healing process.

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Section: Results and Discussionmentioning

confidence: 99%

Multifunctional Casein-Based Wound Dressing Capable of Monitoring and Moderating the Proteolytic Activity of Chronic Wounds

Kolahreez,

Ghasemi-Mobarakeh,

Quartinello

et al. 2024

Biomacromolecules

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“…Fungsi peptida selain dapat merangsang respons fisiologis yang bermanfaat dalam tubuh meliputi aktivitas antimikroba, antihipertensi, antitrombotik, antioksidan, dan imunomodulator [18], [19] dan juga menghadirkan banyak keuntungan seperti toksisitas rendah dan risiko efek samping yang minimal [20]- [22]. Sebagian besar peptida ini dikodekan dalam rantai polipeptida protein dan tetap tidak aktif sampai pembelahan pengikatannya [23]. Dalam pencernaan gastrointestinal, fermentasi protein dan proteolisis eksogen (disebabkan oleh enzim mikroba atau nabati), dapat menghasilkan campuran asam amino dan peptida, yang dapat menghadirkan satu atau lebih aktivitas biologis sesuai dengan tata letak dan ukurannya [15], [24], [25].…”

Section: Hasil Dan Pembahasanunclassified

Review: Peptida Bioaktif Kasein Susu Kambing sebagai Sumber Antimikroba dan Antioksidan

Sinthary,

Arief

2023

J. Sains Kes.

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Penelitian mengenai susu dan produknya dari spesies susu minor 10 tahun terakhir mengalami peningkatan terutama pada kasein susu kambing. Ketertarikan ini awalnya dipengaruhi oleh konsumen intoleran susu sapi sehingga memilih susu kambing sebagai alternatif. Selanjutnya mengalami peninjauan lebih lanjut dimana kasein pada susu kambing merupakan sumber peptida bioaktif potensial. Urutan asam amino berhubungan dengan aktivitas farmakologi ditemukan pada kasein susu sapi dan produk olahannya memiliki kemiripan dengan susu kambing. Keunggulan kasein susu kambing yang mengalami berbagai modifikasi pasca-translasi mengarah pada produksi berbagai macam molekul kasein. Fraksi kasein ini di fragmentasi terutama secara enzimatik untuk mendapatkan peptida bioaktif telah dilaporkan memiliki berbagai aktivitas khususnya sebagai antimikroba dan antioksidan.

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“…Over the years, different animal protein sources have been investigated for bioactive peptide production owing to their high concentration of essential amino acids and bioavailability (Wen et al, 2019). These animal sources include bovine milk (Silva et al, 2019b), goat milk (Nascimento et al, 2021b), whey (Amorim et al, 2018), buffalo cheese (Moura et al, 2016), collagen (Hexsel et al, 2017), snake venom (Alberto- Silva et al, 2021), shrimp (Proksch et al, 2014), fish (Quadros et al, 2019), and industrial waste (Bassan et al, 2016).…”

Section: Researchers Affiliated With the Institutions In São Paulomentioning

confidence: 99%

“…The first study related to the bioactive properties of a peptide was published in 1950 when the receptor-activation ability for umami taste was reported (Chakrabarti et al, 2014). Numerous studies have since been conducted to explore new activities in various bioactive peptides, such as antioxidant, antimicrobial, immunomodulatory, antihypertensive, anticancer, cholesterolemic, and antidiabetic activities (Alberto- Silva et al, 2021;Nascimento et al, 2021b;Santos et al, 2021;Silva et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ten years of research on bioactive peptides in Brazil: a scientometric analysis

SILVA,

BARBOSA,

MARINHO

et al. 2023

Food Sci. Technol (Campinas)

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Peptides are biomolecules composed of amino acids linked through peptide bonds present in proteins. These peptides may have biological activities associated with their secondary structure, especially when released from the original protein sequence. The prospect of these biomolecules is the subject of study and research in various sectors, including the food and pharmaceutical industries. This is due to the possibility of using them as substitutes for traditionally marketed drugs, especially those with restrictive legislation on their use, or losing their effectiveness. A scientometric analysis of studies published from 2011 to 2021 by Brazilian researchers demonstrated the growing interest in basic research involving bioactive peptides from numerous protein sources, including food-based and agro-industrial residues. The antioxidant activity, followed by the antimicrobial potential, has been the most studied property of these biomolecules. Several other activities such as anxiolytic, anti-adipogenic, anticoagulant, anticonvulsant, antisclerotic, and cytoregulatory actions have been reported in a limited number of studies, highlighting the potential application of these biomolecules in the development of new products.

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Antarctic fungus proteases generate bioactive peptides from caseinate

Cited by 11 publications

References 48 publications

Multifunctional Casein-Based Wound Dressing Capable of Monitoring and Moderating the Proteolytic Activity of Chronic Wounds

Multifunctional Casein-Based Wound Dressing Capable of Monitoring and Moderating the Proteolytic Activity of Chronic Wounds

Review: Peptida Bioaktif Kasein Susu Kambing sebagai Sumber Antimikroba dan Antioksidan

Ten years of research on bioactive peptides in Brazil: a scientometric analysis

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