Antarctic ice core samples: culturable bacterial diversity

Shivaji, Sisinthy; Begum, Zareena; Rao, Singireesu Soma Shiva Nageswara; Reddy, Puram V. Vishnu Vardhan; Manasa, Poorna; Sailaja, B.; Prathiba, Mambatta S.; Thamban, Meloth; Krishnan, K. P.; Singh, Shiv Mohan; Srinivas, T. N. R.

doi:10.1016/j.resmic.2012.09.001

Cited by 34 publications

(21 citation statements)

References 33 publications

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“…was previously isolated from Antarctic water and its best growth was observed at 20°C (Nam and Ahn, 2011). Current data supports earlier reports (Neufeld et al, 2004;Steven et al, 2007;Shivaji et al, 2013) that phylum proteobacter are common in cold habitats. In general, G+C content of the bacteria ranges from 16% to more than 75%, which is due to difference in mutation pattern in bacteria (Lightfield et al, 2011).…”

Section: Discussionsupporting

confidence: 90%

“…Bacteria grew between 4°C and 37°C are reported to be psychrotolerant (Reddy et al, 2009), and in our present study all the isolates were grown at 4°C, 20°C and 37°C. Similar study, was done from Antarctic land sample and the authors reported that psychrotolerant species (80%) are dominant than psychrophiles (20%) in Antarctic ice core region (Shivaji et al, 2013;Oh et al, 1991;Kogure, 1998). Isolates were able to grow without NaCl and can tolerate NaCl up to 13% (w/v) and a few of them even tolerate at 13.5% (w/v).…”

Section: Discussionsupporting

confidence: 62%

“…After filtration, membrane was washed in 5 mL of autoclaved sea water. 50 µL from the above 5 mL seawater was used as inoculum and spreaded on Antarctic Bacterial Medium (ABM) (Shivaji et al, 2013) (peptone, 5 g; yeast extract, 2 g; and agar, 20 g; volume make up to 1 L with sea water) and incubated for 15-20 d at 20°C. Bacteria with different morphology and colour were obtained and tabulated in tabular form.…”

Section: Isolation Of Bacteriamentioning

confidence: 99%

“…Esterase activity was confirmed by streaking bacteria on ABM containing tributyrin as a substrate. Lipase and urease test was also performed (Shivaji et al, 2013). Catalase test was performed by adding hydrogen peroxide to loop of culture taken on a slide (Holding and Collee, 1971).…”

Section: Biochemical Characterizationmentioning

confidence: 99%

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Phylogenetic analysis and biological characteristic tests of marine bacteria isolated from Southern Ocean (Indian sector) water

et al. 2015

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Fifty-seven bacteria were isolated from Southern Ocean (Indian sector) water samples which were collected from different latitude and longitude of the ocean. All the isolates were able to grow at 4°C, 20°C, 37°C and tolerable NaCl concentration up to 13.5% (w/v). 29 out of 57 isolates were identified using 16S rDNA amplification and the sequences were submitted to National Center for Biotechnology Information (NCBI). All the isolates were classified by using Ribosomal Database Project (RDP) and found that isolates belongs to Proteobacteria and Bacteriodes. The average G+C content was 56.4%. The isolates were screened for the presence of extracellular enzymes, viz. amylase, catalase, urease, esterase, lipase and protease. The disc diffusion method is used to screen antibiotic production by the isolates against four pathogenic bacteria, viz. Salmonella typhimurium (NCIM 2501), Staphylococcus aureus (NCIM 2122), Bacillus subtilis (NCIM 2193), and Pseudomonas aeruginosa (NCIM 2036). Nine out of 29 were found to be antibiotic producer.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 62%

Section: Isolation Of Bacteriamentioning

confidence: 99%

Section: Biochemical Characterizationmentioning

confidence: 99%

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Phylogenetic analysis and biological characteristic tests of marine bacteria isolated from Southern Ocean (Indian sector) water

et al. 2015

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“…However, there is very limited information available on staphylococcal isolates from Antarctica. Researchers have occasionally isolated staphylococcal strains from Antarctic environmental samples [2][3][4][5][6] and also from several animals obtained during their health evaluation, but they were mostly only classified to the genus level, such as isolates from whale wound lesions [7], a fish stomach [8], lesions on two dead Adélie penguins [9], or skin swabs of Weddell seals [10].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Staphylococcus intermedius Group Isolates Associated with Animals from Antarctica and Emended Description of Staphylococcus delphini

et al. 2020

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Members of the genus Staphylococcus are widespread in nature and occupy a variety of niches, however, staphylococcal colonization of animals in the Antarctic environment has not been adequately studied. Here, we describe the first isolation and characterization of two Staphylococcus intermedius group (SIG) members, Staphylococcus delphini and Staphylococcus pseudintermedius, in Antarctic wildlife. Staphylococcus delphini were found exclusively in Adélie penguins. The report of S. pseudintermedius from Weddell seals confirmed its occurrence in all families of the suborder Caniformia. Partial RNA polymerase beta-subunit (rpoB) gene sequencing, repetitive PCR fingerprinting with the (GTG)5 primer, and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry gave consistent identification results and proved to be suitable for identifying SIG members. Comparative genomics of S. delphini isolates revealed variable genomic elements, including new prophages, a novel phage-inducible chromosomal island, and numerous putative virulence factors. Surface and extracellular protein distribution were compared between genomes and showed strain-specific profiles. The pathogenic potential of S. delphini was enhanced by a novel type of exfoliative toxin, trypsin-like serine protease cluster, and enterotoxin C. Detailed analysis of phenotypic characteristics performed on six Antarctic isolates of S. delphini and eight reference strains from different animal sources enabled us to emend the species description of S. delphini.

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Taxonomic characterization and the bio‐potential of bacteria isolated from glacier ice cores in the High Arctic

Singh

Roy

2015

J. Basic Microbiol.

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Glacier ice and firn cores have ecological and biotechnological importance. The present study is aimed at characterizing bacteria in crustal ice cores from Svalbard, the Arctic. Counts of viable isolates ranged from 10 to 7000 CFU/ml (mean 803 CFU/ml) while the total bacterial numbers ranged from 7.20 × 10(4) to 2.59 × 10(7) cells ml(-1) (mean 3.12 × 10(6) cells ml(-1) ). Based on 16S rDNA sequence data, the identified species belonged to seven species, namely Bacillus barbaricus, Pseudomonas orientalis, Pseudomonas oryzihabitans, Pseudomonas fluorescens, Pseudomonas syncyanea, Sphingomonas dokdonensis, and Sphingomonas phyllosphaerae, with a sequence similarity ranging between 93.5 and 99.9% with taxa present in the database. The isolates exhibited unique phenotypic properties, and three isolates (MLB-2, MLB-5, and MLB-9) are novel species, yet to be described. To the best of our knowledge, this is the first report on characterization of cultured bacterial communities from Svalbard ice cores. We conclude that high lipase, protease, cellulase, amylase, and urease activities expressed by most of the isolates provide a clue to the potential industrial applications of these organisms. These microbes, producing cold-adapted enzymes may provide an opportunity for biotechnological research.

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Antarctic ice core samples: culturable bacterial diversity

Cited by 34 publications

References 33 publications

Phylogenetic analysis and biological characteristic tests of marine bacteria isolated from Southern Ocean (Indian sector) water

Phylogenetic analysis and biological characteristic tests of marine bacteria isolated from Southern Ocean (Indian sector) water

Characterization of Staphylococcus intermedius Group Isolates Associated with Animals from Antarctica and Emended Description of Staphylococcus delphini

Taxonomic characterization and the bio‐potential of bacteria isolated from glacier ice cores in the High Arctic

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