Antarctic Krill Oil Ameliorates Monosodium Iodoacetate-Induced Irregularities in Articular Cartilage and Inflammatory Response in the Rat Models of Osteoarthritis

Abstract

The aim of this study was to examine the effects of Antarctic krill oil (FJH-KO) in a rat model of monosodium iodoacetate (MIA) induced osteoarthritis. The effect of FJH-KO on the development and severity of MIA-induced osteoarthritis was assessed using hematoxylin and eosin (H&E) staining and micro-CT. The expression of PGE2, pro-inflammatory cytokines (IL-1β, TNF-α), and arthritics related genes in osteoarthritic rats in response to FJH-KO supplementation was investigated using real time PCR. FJH-KO supplementation in the arthritic rat model reduced tissue damage, cartilage degeneration, and reduced the MIA-induced irregularities in articular cartilage surface. Serum PGE2, IL-1β, IL-6, and TNF-α levels were higher in MIA treated animals, but these levels decreased upon FJH-KO supplementation. When FJH-KO was provided at a dose of 150 mg/kg b.w to MIA-treated animals, it significantly increased the mRNA expression of anabolic factors. The mRNA expression of catabolic factors was significantly decreased MIA-treated animals that were provided FJH-KO at a dose of 100 and 150 mg/kg b.w. Moreover, the mRNA expression of inflammatory mediators was significantly decreased MIA-treated animals supplemented with FJH-KO. These results suggest supplementation with FJH-KO ameliorates the irregularities in articular cartilage surface and improves the inflammatory response in the osteoarthritis. Thus, FJH-KO could serve as a potential therapeutic agent for osteoarthritis treatment.

Effects of Palm Tocotrienol-Rich Fraction Alone or in Combination with Glucosamine Sulphate on Grip Strength, Cartilage Structure and Joint Remodelling Markers in a Rat Model of Osteoarthritis

“…In another study, MIA arrested chondrocyte proliferation due to S-phase arrest [28]. MIA has been commonly used to induce cartilage damage in animals to mimic osteoarthritis in humans [29][30][31]. In this study, MIA induced apparent cartilage damage, as similarly observed in previous studies [19,32].…”

Antarctic Krill Oil from Euphausia superba Ameliorates Carrageenan-Induced Thrombosis in a Mouse Model

“…The extracts were stored at −20 • C until use. The nutritional components of FJH-KO were the same as those reported in previous studies, and the sum of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in FJH-KO was 204.49 ± 2.72 mg/g [20,21].…”

“…Several studies have reported that the consumption of n-3 PUFAs can decrease the platelet-mediated generation of thrombin and reduce factors that contribute to blood clot formation [18,19]. Previous studies have reported that FJH-KO supplementation attenuates the inflammatory response of chondrocytes in an animal model of osteoarthritis [20,21]. However, the antithrombotic effect of FJH-KO has not yet been studied.…”

Anti-Osteoarthritic Effects of Antarctic Krill Oil in Primary Chondrocytes and a Surgical Rat Model of Knee Osteoarthritis

“…Previous randomized controlled trials have reported that dietary KO reduces subjective arthritic symptoms and systemic inflammation in patients with chronic inflammation including OA [30], and improves joint pain and stiffness in patients with mild to moderate knee OA [31,32]. Dietary supplementation of KO has shown beneficial effects through its anti-inflammatory properties in animal models of monosodium iodoacetate (MIA)-induced knee OA [33], rheumatoid arthritis [34], low-grade inflammation [35], and obesity [36,37]. Moreover, the oral administration of KO ameliorates joint cartilage degeneration by activating chondrocyte autophagy and inhibiting apoptosis in a surgical mouse model of knee OA [38].…”