Ante mortem diagnosis of mycobacterial infection by liver biopsy in a budgerigar (Melopsittacus undulatus)

Foldenauer, Ulrike; Curd, S; Zulauf, I; Hatt, Jean‐Michel

doi:10.1024/0036-7281.149.6.273

Cited by 7 publications

(5 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4, 26 We report the results of an uncontrolled clinical study undertaken to determine whether a recommended multidrug treatment regimen consisting of azithromycin, ethambutol, and rifampin administered orally 4,9,12,14,21,26 during a period of 6 months will eliminate infection from ring-neck doves (Streptopelia risoria) naturally infected with M avium subsp avium. We also sought to determine whether this treatment protocol would be free of adverse effects, if the mycobacteria would develop antibiotic resistance, and if a liver biopsy at the end of treatment, as has been previously reported, 14,27 could be used to assess treatment success.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Multidrug Therapy With Azithromycin, Rifampin, and Ethambutol for the Treatment ofMycobacterium aviumsubspaviumin Ring-neck Doves (Streptopelia risoria): An Uncontrolled Clinical Study

Saggese¹,

Tizard²,

Gray³

et al. 2014

Journal of Avian Medicine and Surgery

View full text Add to dashboard Cite

An uncontrolled clinical study was conducted to evaluate the efficacy of a multidrug protocol in 16 ring-neck doves ( Streptopelia risoria ) from a flock naturally infected with Mycobacterium avium subsp avium. The doves were considered infected on the basis of a high prevalence of infection in a group of 29 birds that were euthanatized from the same flock and clinical signs and pathologic results consistent with infection in the remaining birds. All birds were treated with azithromycin (43 mg/kg), rifampin (45 mg/kg), and ethambutol (30 mg/kg) administered orally once daily for 180 days. Five birds died during treatment and were confirmed positive for mycobacteriosis on postmortem examination. Of the remaining 11 birds, infection and disease were present in 9 (81.8%) at the end of the treatment. Postmortem investigation showed that 2 mycobacterial isolates were resistant to ethambutol, intermediately sensitive to rifampin, and sensitive to azithromycin. Microscopic examination of liver sections equivalent of those that would be taken for biopsy showed that biopsy as a method of monitoring birds for treatment success had poor sensitivity. Toxicity associated with drug therapy was not observed in these doves nor in 6 outwardly healthy ring-neck doves exposed to the same treatment. The results of this study showed that this protocol of azithromycin, rifampin, and ethambutol has poor efficacy when administered for 180 days for treatment of doves infected with M avium subsp avium.

show abstract

Section: Introductionmentioning

confidence: 99%

Evaluation of Multidrug Therapy With Azithromycin, Rifampin, and Ethambutol for the Treatment ofMycobacterium aviumsubspaviumin Ring-neck Doves (Streptopelia risoria): An Uncontrolled Clinical Study

Saggese¹,

Tizard²,

Gray³

et al. 2014

Journal of Avian Medicine and Surgery

View full text Add to dashboard Cite

show abstract

“…Antemortem diagnoses are rare and in majority of cases are the result of biopsy of the organs with lesions, direct Ziehl-Nielsen stained smears and culture (Kiehn et al, 1996;Washko et al, 1998;Clippinger at al., 1998;Foldenauer et al, 2007). Despite the lack of calcification of tubercles in birds, radiography is also recognized as a useful diagnostic tool (Rosskopf et al, 1981;Clippinger et al, 1998;Tell et.…”

mentioning

confidence: 99%

Experimental infection of budgerigars (Melopsittacus undulatus) with fiveMycobacteriumspecies

Ledwoń

Szeleszczuk

Zwolska³

et al. 2008

Avian Pathology

View full text Add to dashboard Cite

International audienceThe aim of the present study was to determine the susceptibility of budgerigars (Melopsittacus undulatus) to infections with different Mycobacterium species. For inoculations the following M. species were used: M. avium subsp. avium, M. bovis subsp. bovis, M. tuberculosis subsp. tuberculosis, M. intracellulare and M. fortuitum subsp. fortuitum. The bacterial suspension was administrated intramuscularly and all the birds were monitored for 70 days starting from the day of inoculation. During the experiment clinical examination, x- rays, plate agglutination tests, tuberculin tests, feces smear preparations and culture of mycobacteria were performed. The study showed, that M. bovis subsp. bovis was the most pathogenic M. species for budgerigars. After inoculation the bacilli induced tuberculosis-typical, clinical signs and necropsy findings. In two out of six birds infected with M. bovis subsp. bovis radiological changes were also visible. Birds inoculated with other M. species did not show any typical symptoms of infection and only the results of histopathological and bacteriological examinations indicated the presence of infection

show abstract

“…M. caprae was found to be present in the biopsy material from two out of four infected EB; the first case of biopsy being used in EB for this purpose. In humans, biopsy is sometimes included in the testing for tuberculosis [36], while in animals, it has been performed for various reasons, such as collecting material for detecting mycobacteriosis in birds [37].…”

Section: Discussionmentioning

confidence: 99%

Biopsy and Tracheobronchial Aspirates as Additional Tools for the Diagnosis of Bovine Tuberculosis in Living European Bison (Bison bonasus)

Didkowska

Orłowska

Witkowski

et al. 2020

Animals

View full text Add to dashboard Cite

The diagnosis of bovine tuberculosis (BTB) in living wildlife remains a complex problem, and one of particular importance in endangered species like European bison (Bison bonasus). To identify infection and avoid the unnecessary culling of such valuable individuals, current best practice requires the collection and culture of material from living animals, as mycobacteria isolation remains the gold standard in BTB diagnosis. However, such isolation is challenging due to the need for the immobilization and collection of appropriate clinical material, and because of the sporadic shedding of mycobacteria. In the present study, we evaluated the potential of sampling for the detection of BTB in a group of seven living European bison suspected of being infected with Mycobacterium caprae. The specimens were collected both as swabs from the nasal and pharyngeal cavities, tracheobronchial aspirates (TBA), ultrasound-guided biopsies from lateral retropharyngeal lymph nodes, and post mortem, from mandibular, retropharyngeal and mediastinal lymph nodes. Clinical samples were tested for mycobacterial species via mycobacteriological culture and PCR. M. caprae was isolated from collected material in two out of four living infected individuals (TBA, biopsy) and mycobacterial DNA was detected in three out of four (TBA, pharyngeal swab) bison. This is the first report of isolation of M. caprae in living European bison. Our findings demonstrate the value of diagnostic tests based on both molecular testing and culture in European bison and confirm the respiratory shedding of viable M. caprae in this host species.

show abstract

Ante mortem diagnosis of mycobacterial infection by liver biopsy in a budgerigar (Melopsittacus undulatus)

Cited by 7 publications

References 4 publications

Evaluation of Multidrug Therapy With Azithromycin, Rifampin, and Ethambutol for the Treatment ofMycobacterium aviumsubspaviumin Ring-neck Doves (Streptopelia risoria): An Uncontrolled Clinical Study

Evaluation of Multidrug Therapy With Azithromycin, Rifampin, and Ethambutol for the Treatment ofMycobacterium aviumsubspaviumin Ring-neck Doves (Streptopelia risoria): An Uncontrolled Clinical Study

Experimental infection of budgerigars (Melopsittacus undulatus) with fiveMycobacteriumspecies

Biopsy and Tracheobronchial Aspirates as Additional Tools for the Diagnosis of Bovine Tuberculosis in Living European Bison (Bison bonasus)

Contact Info

Product

Resources

About