Antennal expressed genes of the yellow fever mosquito (Aedes aegypti L.); characterization of odorant-binding protein 10 and takeout

Bohbot, Jonathan D.; Vogt, Richard G.

doi:10.1016/j.ibmb.2005.03.010

Cited by 85 publications

(79 citation statements)

References 111 publications

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“…Mean ± s.e.m. (Bohbot and Vogt, 2005;Du et al, 2003;Sarov-Blat et al, 2000). To investigate this hypothesis further, we studied the sexual dimorphism of locomotor activity, which we have previously reported to be linked to JH (Belgacem and Martin, 2002).…”

Section: The Journal Of Experimental Biologymentioning

confidence: 99%

Regulation of feeding behaviour and locomotor activity bytakeoutinDrosophila

Meunier

Belgacem

Martin

2007

Journal of Experimental Biology

140

143

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SUMMARY The hormonal regulation of feeding behaviour is well known in vertebrates,whereas it remains poorly understood in insects. Here, we report that the takeout gene is an essential component of nutritional homeostasis in Drosophila. takeout encodes a putative juvenile hormone (JH)binding protein and has been described as a link between circadian rhythm and feeding behaviour. However, the physiological role of takeout and its putative link to JH remain unknown. In this study, we show that takeout (to1) flies failed to adapt their food intake according to food availability and that most defects could be genetically rescued. When food is abundant, to1 are hyperphagic, yielding to hypertrophy of the fat body. When food reappears after a starvation period, to1 flies do not increase their food intake as much as wild-type flies. This defect in food intake regulation is partly based on the action of Takeout on taste neurons, because the sensitivity of to1 gustatory neurons to sugars does not increase after starvation, as in wild-type neurons. This lack of regulation is also evident at the locomotor activity, which normally increases during starvation, a behaviour related to food foraging. In addition, to1 flies lack sexual dimorphism of locomotor activity,which has previously been linked to the JH circulating level. Moreover,application of the JH analog methoprene rescues the phenotype. These results suggest that takeout plays a central role as a feeding regulator and may act by modulating the circulating JH level.

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Section: The Journal Of Experimental Biologymentioning

confidence: 99%

Regulation of feeding behaviour and locomotor activity bytakeoutinDrosophila

Meunier

Belgacem

Martin

2007

Journal of Experimental Biology

140

143

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“…On the other hand, the homology to JHBP and the potential role of takeout homologs as small molecule binding proteins or carriers in antennae (Robertson et al, 1999;Bohbot and Vogt, 2005), and exocrine glands (Hojo et al, 2005), suggest that the CA proteins act as intracellular JH or JH precursor carrier proteins. This intriguing possibility calls for a new look at metabolite trafficking in these endocrine glands.…”

Section: Takeout/jhbp Homolog and Retinol-binding Protein Homologmentioning

confidence: 99%

Comparative genomics of insect juvenile hormone biosynthesis☆

Noriega

Ribeiro

Koener

et al. 2006

Insect Biochemistry and Molecular Biology

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The biosynthesis of insect juvenile hormone (JH) and its neuroendocrine control are attractive targets for chemical control of insect pests and vectors of disease. To facilitate the molecular study of JH biosynthesis, we analyzed ESTs from the glands producing JH, the corpora allata (CA) in the cockroach Diploptera punctata, an insect long used as a physiological model species and compared them with ESTs from the CA of the mosquitoes Aedes aegypti and Anopheles albimanus. The predicted genes were analyzed according to their probable functions with the Gene Ontology classification, and compared to Drosophila and Anopheles gambiae genes. A large number of reciprocal matches in the cDNA libraries of cockroach and mosquito CA were found. These matches defined known and suspected enzymes of the JH biosynthetic pathway, but also several proteins associated with signal transduction that might play a role in the modulation of JH synthesis by neuropeptides. The identification in both cockroach and mosquito CA of homologs of the small ligand binding proteins from insects, Takeout/JH binding protein and retinol-binding protein highlights a hitherto unsuspected complexity of metabolite trafficking, perhaps JH precursor trafficking, in these endocrine glands. Furthermore, many reciprocal matches for genes of unknown function may provide a fertile ground for an in-depth study of allatal-specific cell physiology.

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“…At least 80 To genes have now been identified from seven orders of insect, including 20 from D. melanogaster, but few have been characterized in any detail. Due to the localization and/or expression profiles of their transcripts, some are proposed to have roles in chemosensory perception (5)(6)(7)(8), whereas others are thought to be involved in binding terpenoids (9), including juvenile hormones (JH) (10 -15).…”

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confidence: 99%

Crystal Structure of Epiphyas postvittana Takeout 1 with Bound Ubiquinone Supports a Role as Ligand Carriers for Takeout Proteins in Insects

Hamiaux

Stanley

Greenwood

et al. 2009

Journal of Biological Chemistry

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Takeout (To) proteins are found exclusively in insects and have been proposed to have important roles in various aspects of their physiology and behavior. Limited sequence similarity with juvenile hormone-binding proteins (JHBPs), which specifically bind and transport juvenile hormones in Lepidoptera, suggested a role for To proteins in binding hydrophobic ligands. We present the first crystal structure of a To protein, EpTo1 from the light brown apple moth Epiphyas postvittana, solved in-house by the single-wavelength anomalous diffraction technique using sulfur anomalous dispersion, and refined to 1.3 Å resolution. EpTo1 adopts the unusual ␣/␤-wrap fold, seen only for JHBP and several mammalian lipid carrier proteins, a scaffold tailored for the binding and/or transport of hydrophobic ligands. EpTo1 has a 45 Å long, purely hydrophobic, internal tunnel that extends for the full length of the protein and accommodates a bound ligand. The latter was shown by mass spectrometry to be ubiquinone-8 and is probably derived from Escherichia coli. The structure provides the first direct experimental evidence that To proteins are ligand carriers; gives insights into the nature of endogenous ligand(s) of EpTo1; shows, by comparison with JHBP, a basis for different ligand specificities; and suggests a mechanism for the binding/release of ligands. Takeout (To) 3 family have been proposed to have important roles in various aspects of insect physiology and behavior and as such may represent novel targets for insect control strategies. The original member, To, was discovered in Drosophila melanogaster as a clock-regulated gene, acting as a molecular link between circadian rhythms and feeding behavior (1, 2). In addition, To expression is regulated by the somatic sex determination pathway and affects male courtship behavior (3, 4). To has a predicted secretion signal (1) and is secreted in the hemolymph of adult males (4). At least 80 To genes have now been identified from seven orders of insect, including 20 from D. melanogaster, but few have been characterized in any detail. Due to the localization and/or expression profiles of their transcripts, some are proposed to have roles in chemosensory perception (5-8), whereas others are thought to be involved in binding terpenoids (9), including juvenile hormones (JH) (10 -15). Members of theTo proteins share limited sequence similarity and a conserved N-terminal disulfide bond with juvenile hormone-binding proteins (JHBPs). These proteins specifically bind and transport JH to target tissues in the Lepidoptera, further suggesting a role for To proteins in binding hydrophobic ligands (2). There are, however, two major differences between the families: a second disulfide bond in the JHBPs, not present in To; and the presence of two conserved C-terminal sequence motifs that are unique to the To family (2). Very recently, the structure of JHBP from the moth Galleria mellonella (GmJHBP) has been solved, revealing an unusual fold in which a highly curved antiparallel ␤-sheet wraps around a...

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Antennal expressed genes of the yellow fever mosquito (Aedes aegypti L.); characterization of odorant-binding protein 10 and takeout

Cited by 85 publications

References 111 publications

Regulation of feeding behaviour and locomotor activity bytakeoutinDrosophila

Regulation of feeding behaviour and locomotor activity bytakeoutinDrosophila

Comparative genomics of insect juvenile hormone biosynthesis☆

Crystal Structure of Epiphyas postvittana Takeout 1 with Bound Ubiquinone Supports a Role as Ligand Carriers for Takeout Proteins in Insects

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