Antennal morphology and sensilla ultrastructure of Ascia monuste (Linnaeus) (Lepidoptera: Pieridae)

Limberger, Guilherme Martins; Brugnera, Ricardo; Fonseca, Duane Barros da

doi:10.1016/j.micron.2020.103000

Cited by 10 publications

(5 citation statements)

References 26 publications

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“…We found that the EAG responses to low and high concentrations of α-pinene in males were higher than those in females (Table 1), indicating that males are inherently more sensitive to some odour compounds than females. In addition, the perception of food odours by adult butterflies is thought to be mediated by a combination of sensilla squamiformia, sensilla trichodea, and sensilla coeloconica on the antennae [23,24]. In this study, we found that the number of sensilla trichodea and sensilla coeloconica in the antennae of T. limniace males was higher than that of females; thus, the number of antennal sensilla in males and females may be associated with their learning differences.…”

Section: Discussionmentioning

confidence: 49%

“…The antennae were washed for 30 s using 70% ethanol solution and stove-dried at 40 • C for 24 h. After drying, the specimens were attached to a holder using electric adhesive tape, sputter-coated with gold for 45 s (Hitachi e-1010, Tokyo, Japan), and examined and photographed with an S-3000 N SEM (at 16 kV, Hitachi, Tokyo, Japan). The different sensilla types were discriminated based on their morphological features described in the literature [22][23][24]. The abundance of different sensilla types of T. limniace varied, and, thus, we counted the sensilla on each segment from the dorsal, ventral, and lateral parts of the antenna.…”

Section: Scanning Electron Microscopementioning

confidence: 99%

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Pupal and Adult Experience Affect Adult Response to Food Odour Components in the Flower-Visiting Butterfly Tirumala limniace

Li,

Wang,

Bian

et al. 2024

Insects

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Butterflies have the ability to learn to associate olfactory information with abundant food sources during foraging. How the co-occurrence of both food and food odours affects the learning behaviour of adults and whether butterflies perceive the odour of their surroundings and develop a preference for that odour during the pupal stage have rarely been tested. We examined the effect of experience with food odour components (α-pinene and ethyl acetate) during the pupal and adult stages on the foraging behaviour of the flower-visiting butterfly Tirumala limniace. We found that α-pinene exposure during the pupal stage changed the foraging preference of newly emerged adults. T. limniace exhibits olfactory learning in the adult stage, and adult learning may influence their previous pupal memory. Moreover, adults’ odour preference did not continue to increase over multiple training times. The learning ability of adults for floral odours (α-pinene) was greater than that for non-floral odours (ethyl acetate). In contrast to previous studies, we found that males learned odours more efficiently than females did. This could be attributed to differences in antennal sensilla, affecting sensitivity to compounds and nectar demand between males and females. Our study provides further insight into how olfactory learning helps flower-visiting butterflies use food odours to forage better.

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Section: Discussionmentioning

confidence: 49%

Section: Scanning Electron Microscopementioning

confidence: 99%

Pupal and Adult Experience Affect Adult Response to Food Odour Components in the Flower-Visiting Butterfly Tirumala limniace

Li,

Wang,

Bian

et al. 2024

Insects

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“…Similarly, the sulci, where the ST clusters occur, do not always appear in pairs. In A. monuste (Linnaeus), each flagellomere has only one sulcus on its venter [21]. In other butterfly genera, such as Parnara and Pelopidas of the Hesperiidae, STs also cluster into square sulci or do not form a sulcus [32].…”

Section: Discussionmentioning

confidence: 99%

“…Sensilla were identified and categorized according to the terminology described by Boddum et al, Crook and Mordue, Schneider, and Limberger et al [18][19][20][21]. The androconial scales in scent patches were described according to the wing scale terminology of Ghiradella [22].…”

Section: Terminologymentioning

confidence: 99%

Morphological Characterization of the Antenna and Scent Patch of Three Danaus Species (Papilionoidea: Nymphalidae, Danainae)

Yang,

Ding,

Wang

et al. 2024

Insects

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The scent system of Danaus is important for the study of butterfly sexual communication and relevant investigations in biomimetics due to its involvement with mimicry. Using light, scanning, and transmission electron microscopy, the morphological characteristics of Danaus’ antennae and scent patches of the scent system for three species, D. chrysippus, D. genutia, and D. plexippus, were investigated herein. Their apical clubs of the flagellums contain sensilla trichodea, sensilla chaetica, and sensilla coeloconica. The scent patch scales typically have a tree-like structure in its lumen at the nano-scale. Comparisons were made between the androconial scales and the other scales in scent patches. Rank sum tests showed significant differences in scent patch scales’ characteristics between the species, as well as in the ultrastructure of antennal segments between species and sexes. Spearman’s correlation tests showed significant correlations between the morphological characteristics of androconial scales in scent patches. Moreover, the antennal characteristics were significantly correlated. The morphological characteristics of the females’ antennae were significantly correlated with those of the males’ antennae and androconial scales. However, the significance and coefficient of these correlations were inconsistent across species and sexes. This study provides fundamental morphological information that helps in understanding the pheromone recognition system of Danaus.

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“…Insects have evolved a sophisticated olfactory system to accomplish many essential behaviors, such as locating the mates, choosing the best nutrition‐ and oviposition‐host plants, and avoiding predators (Benton, 2009; J. J. Zhou, 2010). Antennae are specialized organs of insect for olfaction, and the surface is covered with different types of sensilla (Limberger et al, 2021). Olfactory receptor neurons (ORNs) extended theirs dendrites into the sensilla lymph where peripheral olfactory signal transduction occur.…”

Section: Introductionmentioning

confidence: 99%

Functional analysis of the odorant receptor coreceptor in odor detection in Grapholita molesta (lepidoptera: Tortricidae)

Xiu-lin

Chen

et al. 2021

Arch Insect Biochem Physiol

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The olfactory system must detect and discriminate various semiochemicals in the environment. In response to such diversity, insects have evolved a family of odorant‐gated ion channels composed of a common receptor (coreceptor, Orco) and a ligand‐binding tuning odorant receptor (OR) that confers odour specificity. This study aims to examine the expression pattern of Orco gene of Grapholita molesta (GmolOrco) and to elucidate the role of GmolOrco in detecting G. molesta sex pheromone and green leaf volatiles by using gene silencing via RNA interference (RNAi) coupled antennal electrophysiological (EAG). Multiple sequence alignment showed that GmolOrco shared high sequence similarities with the Orco ortholog of lepidopterans. The results of real‐time quantitative PCR detection demonstrated that GmolOrco was predominantly expressed in adult antennae and had the highest expression quantity in adult period among the different developmental stages. Compared with the noninjected controls, GmolOrco expression in GmolOrcodouble‐stranded RNA (dsRNA)‐injected males was reduced to 39.92% and that in females was reduced to 40.43%. EAG assays showed that the responses of GmolOrco‐dsRNA injected males to sex pheromones (Z)‐8‐dodecenyl acetate (Z8‐12:OAc) and (Z)‐8‐dodecenyl alcohol (Z8‐12:OH) were significantly reduced, and the GmolOrco‐dsRNA‐injected female to green leaf volatile (Z)‐3‐hexenyl acetate also significantly declined. We inferred that Orco‐mediated olfaction was different in male and female G. molesta adults and was mainly involved in recognizing the sex pheromones released by female moths.

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Antennal morphology and sensilla ultrastructure of Ascia monuste (Linnaeus) (Lepidoptera: Pieridae)

Cited by 10 publications

References 26 publications

Pupal and Adult Experience Affect Adult Response to Food Odour Components in the Flower-Visiting Butterfly Tirumala limniace

Pupal and Adult Experience Affect Adult Response to Food Odour Components in the Flower-Visiting Butterfly Tirumala limniace

Morphological Characterization of the Antenna and Scent Patch of Three Danaus Species (Papilionoidea: Nymphalidae, Danainae)

Functional analysis of the odorant receptor coreceptor in odor detection in Grapholita molesta (lepidoptera: Tortricidae)

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