Antennal resolution of pulsed pheromone plumes in three moth species

Bau, Josep; Justus, Kristine A.; Cardé, Ring T.

doi:10.1016/s0022-1910(02)00062-8

Cited by 50 publications

(27 citation statements)

References 30 publications

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“…For example, we can predict that EAG amplitude is reduced by a factor of 10 at ϳ32 Hz in C. cautella and ϳ106 Hz in S. exigua. These estimates agree with previous EAG measurements using regularly pulsed stimuli in a laminar flow wind tunnel in which both species could resolve pheromone pulses up to Ն33 Hz, with S. exigua giving more robust responses at high frequencies (Bau et al 2002).…”

Section: Antennal Resolution Of Pheromone Plume Dynamicssupporting

confidence: 90%

“…Electroantennogram (EAG) experiments with pulsed pheromone plumes in a laminar flow wind tunnel suggested that regularly pulsed stimuli at frequencies Ն30 Hz can be resolved by some moth species (Bau et al 2002). However, as described in the preceding text, pheromone rarely arrives at an antenna as a regularly pulsed signal but rather as an irregular signal with a complex temporal spectrum.…”

Section: Introductionmentioning

confidence: 99%

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Dynamic Properties of Antennal Responses to Pheromone in Two Moth Species

Justus¹,

Cardé²,

French³

2005

Journal of Neurophysiology

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. Dynamic properties of pheromone plumes are behaviorally important in some moths for inducing upwind flight, but little is known about the time-dependent properties of odor transduction or the mechanisms that limit receptor dynamic sensitivity. We stimulated male antennae of two moth species, Cadra cautella and Spodoptera exigua, with pheromone plumes in a wind tunnel while recording electroantennograms (EAG) and concentration of a surrogate plume (propylene, which mimics a pheromone plume) using a photoionization detector (PID). Turbulent plumes were produced by mechanical baffles, creating broad frequency range dynamic concentration changes at the antennae. Frequency response functions and coherence functions between PID and EAG signals were used to measure the dynamic responses of the two species to pheromone blends and individual components. A single time constant filter fitted the responses of both species, but S. exigua was about three times faster than C. cautella. Responses to individual pheromone components were significantly different in S. exigua but not in C. cautella. We also fitted the data with a simple blockstructured nonlinear cascade. This supported the simple filter model but also suggested that the response saturates at an early stage of chemotransduction.

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Section: Antennal Resolution Of Pheromone Plume Dynamicssupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Dynamic Properties of Antennal Responses to Pheromone in Two Moth Species

Justus¹,

Cardé²,

French³

2005

Journal of Neurophysiology

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“…We therefore propose that the activation of OrX-Orco through intracellular signalling is a unique adaptation in the Pterygota to regulate the sensitivity of OrX-Orco heteromers. Future studies comparing olfactory signalling in pterygote (winged) versus apterygote insects, as well as detailed analyses utilizing high-speed tracking of flying insects and simultaneous measurement of plume structure and physiological response during flight behaviour (Vickers et al, 2001;Bau et al, 2002;Van Breugel and Dickinson, 2014) are necessary to test these hypotheses further.…”

Section: Discussionmentioning

confidence: 99%

Intracellular regulation of the insect chemoreceptor complex impacts odor localization in flying insects

Getahun

Thoma

Lavista-Llanos

et al. 2016

Journal of Experimental Biology

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Flying insects are well known for airborne odour tracking and have evolved diverse chemoreceptors. While ionotropic receptors (IRs) are found across protostomes, insect odorant receptors (ORs) have only been identified in winged insects. We therefore hypothesized that the unique signal transduction of ORs offers an advantage for odour localization in flight. Using Drosophila, we found expression and increased activity of the intracellular signalling protein PKC in antennal sensilla following odour stimulation. Odour stimulation also enhanced phosphorylation of the OR co-receptor Orco in vitro, while site-directed mutation of Orco or mutations in PKC subtypes reduced the sensitivity and dynamic range of OR-expressing neurons in vivo, but not IR-expressing neurons. We ultimately show that these mutations reduce competence for odour localization of flies in flight. We conclude that intracellular regulation of OR sensitivity is necessary for efficient odour localization, which suggests a mechanistic advantage for the evolution of the OR complex in flying insects.

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“…(40) With the help of technologies such as electrophysiology, Bau et al (41) studied EAG responses with respect to changes in the impulse frequencies of sex pheromones, the temperature, and the humidity, and they also studied the specificity of insect olfactory responses to the impulse frequencies of sex pheromones. Vickers et al also conducted research on related aspects.…”

Section: Electroantennography (Eag) Techniques For Insectsmentioning

confidence: 99%

Recent Advances in Olfactory Receptor (OR) Biosensors and Cell Signaling Cascade Amplification Systems

2018

Sensors and Materials

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There are a variety of methods to detect odorant molecules, including gas chromatography (GC) and its related coupled techniques, electronic noses, and olfactory receptor (OR)-based biosensors. Nevertheless, studies in which cell and cell signaling amplifier systems were directly utilizied to detect odors are very few. In this review, we aim to provide some references and suggestions for researchers in related fields by summarizing OR sensors and olfactory cell signaling cascade amplifier systems. In this paper, we summarize the detection methods for odorants, introduce the research and progress on OR sensors, and present a proposal of utilizing cell or tissue signaling cascade amplifier systems to amplify electrochemical signals and the use of the G protein signaling cascade amplifier system to prepare electrochemical biosensors. In recent years, the detection technology of electrochemical sensors has matured. Meanwhile, olfactory sensation within organisms basically transfer nerve signals or metabolic endocrine signals in the form of electrochemical signals. Therefore, ORs combined with cell signaling cascade amplifier systems, as well as electrochemical sensors of signal cascade amplifier systems, have incredible prospects for development and application. The proposal of OR electrochemical biosensors and their cell signaling cascade amplifier systems, provides a new idea and a quantitative method for the detection and assessment of odorants and their physiologic processes in the nervous system.

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Antennal resolution of pulsed pheromone plumes in three moth species

Cited by 50 publications

References 30 publications

Dynamic Properties of Antennal Responses to Pheromone in Two Moth Species

Dynamic Properties of Antennal Responses to Pheromone in Two Moth Species

Intracellular regulation of the insect chemoreceptor complex impacts odor localization in flying insects

Recent Advances in Olfactory Receptor (OR) Biosensors and Cell Signaling Cascade Amplification Systems

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