Antennal transcriptome analysis of olfactory genes and tissue expression profiling of odorant binding proteins in Semanotus bifasciatus (cerambycidae: coleoptera)

Abstract

Background Insect olfactory proteins can transmit chemical signals in the environment that serve as the basis for foraging, mate searching, predator avoidance and oviposition selection. Semanotus bifasciatus is an important destructive borer pest, but its olfactory mechanism is not clear. We identified the chemosensory genes of S. bifasciatus in China, then we conducted a phylogenetic analysis of the olfactory genes of S. bifasciatus and other species. And the expression profiles of odorant binding proteins (OBPs) genes in different tissues and different genders of S. bifasciatus were determined by quantitative real-time PCR for the first time. Results A total of 32 OBPs, 8 chemosensory proteins (CSPs), 71 odorant receptors (ORs), 34 gustatory receptors (GRs), 18 ionotropic receptors (IRs), and 3 sensory neuron membrane proteins (SNMPs) were identified. In the tissue expression analysis of OBP genes, 7 OBPs were higher expressed in antennae, among them, SbifOBP2, SbifOBP3, SbifOBP6, SbifOBP7 and SbifOBP20 were female-biased expression, while SbifOBP1 was male-biased expression and SbifOBP22 was no-biased expression in antennae. In addition, the expressed levels of SbifOBP4, SbifOBP12, SbifOBP15, SbifOBP27 and SbifOBP29 were very poor in the antennae, and SbifOBP4 and SbifOBP29 was abundant in the head or legs, and both of them were male-biased expression. While SbifOBP15 was highly expressed only at the end of the abdomen with its expression level in females three times than males. Other OBPs were expressed not only in antennae but also in various tissues. Conclusion We identified 166 olfactory genes from S. bifasciatus, and classified these genes into groups and predicted their functions by phylogenetic analysis. The majority of OBPs were antenna-biased expressed, which are involved in odor recognition, sex pheromone detection, and/or host plant volatile detection. However, also some OBPs were detected biased expression in the head, legs or end of the abdomen, indicating that they may function in the different physiological processes in S. bifasciatus.

Identification of odorant‐binding proteins and functional analysis of antenna‐specific BhorOBP28 in Batocera horsfieldi (Hope)

“…We detected all previously described BhorOBPs and confirmed the ORFs of all 36 OBPs by molecular cloning and sequencing. Compared with other beetles of the family Cerambycidae, the number of BhorOBPs identified here is less than that in A. glabripennis (61 OBPs) and A. chinensis (46 OBPs), 41,42 and more than that was reported in M. alternatus (29 OBPs), X. quadripes (24 OBPs), and S. bifasciatus (32 OBPs), 43–45 which might be related to their host range and habitat. Phylogenetic analysis revealed that most of the BhorOBPs have a clear ortholog with other OBPs in Cerambycidae.…”

“…A Cerambycidae-based phylogenetic analysis was performed, including 33 OBPs from B. horsfieldi, 48 OBPs from Anoplophora glabripennis, 41 35 OBPs from Anoplophora chinensis, 42 23 OBPs from M. alternatus, 43 23 OBPs from Xylotrechus quadripes, 44 23 OBPs from Semanotus bifasciatus. 45 N-terminal signal peptide sequences were predicted using the SignalP-5.0 server (http:// www.cbs.dtu.dk/services/SignalP/). Mature OBPs' amino acid sequences were aligned using MAFFT multiple sequence alignment (https://www.ebi.ac.uk/Tools/msa/mafft/).…”

A Highly Expressed Antennae Odorant-Binding Protein Involved in Recognition of Herbivore-Induced Plant Volatiles in Dastarcus helophoroides

“…Compared to other coleopteran insects, D. helophoroides showed a relatively average repertoire of OBPs (21 OBPs), which is similar to Holotrichia parallela (25 OBPs) [ 41 ], Harmonia axyridis (19 OBPs) [ 42 ], Colaphellus bowringi (26 OBPs) [ 43 ], and Tenebrio molitor (19 OBPs) [ 44 ]. Meanwhile, more OBPs were found in Anoplophora chinensis (46 OBPs) [ 45 ], Semanotus bifasciatus (32 OBPs) [ 46 ], M. alternatus (29 OBPs) [ 40 ], and T. castaneum (50 OBPs) [ 36 ]. The variation in the number of chemosensory genes could be linked to an adaptation to living habitats and other environmental factors.…”

Ligand binding properties of three odorant‐binding proteins in striped flea beetle Phyllotreta striolata towards two phthalate esters

“…In contrast, PstrOBP13 and PstrOBP16, members of the minus‐C OBP family, did not exhibit marked expression bias. This broad and unspecific expression characteristic of minus‐C OBPs is common to a variety of Coleopteran insects (Li, Hao, et al, 2022; Wu et al, 2022). These expression features were consistent with the classification of PstrOBPs, suggesting that they follow evolutionary laws to perform diverse functions.…”