Antennapedia: The complexity of a master developmental transcription factor

Rosales‐Vega, Marco; Reséndez‐Pérez, Diana; Vázquez, Martha

doi:10.1002/dvg.23561

Cited by 3 publications

(1 citation statement)

References 108 publications

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“…This spatial restriction of expression may arise from the negative regulatory influences exerted by the Ultrabithorax (Ubx) [11]. Antp expression is tightly regulated during development, with two promoters (P1 and P2) targeting mid-thoracic tissue, each influenced by specific enhancers active in early embryogenesis, and various mechanisms modulating larval tissue expression [12].…”

Section: Introductionmentioning

confidence: 99%

CRISPR/Cas9-Mediated Mutagenesis of Antennapedia in Spodoptera frugiperda

Wang,

Zhao,

Liu

et al. 2023

Insects

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The homeotic gene Antennapedia (Antp) has been identified as playing a pivotal role in the morphogenesis of the thorax and wings across various insect species. Leveraging insights from previous studies, the functional characterization of Antp in S. frugiperda was undertaken using RT-qPCR and the CRISPR/Cas9 genome-editing system. Phylogenetic analyses indicate that Antp shares a high degree of sequence homology among Lepidoptera species. The expression profile of SfAntp was detected by RT-qPCR. The results showed that SfAntp was expressed in the whole growth cycle of S. frugiperda, the expression level was the highest in the egg stage, and the expression level was higher from 12 h to 48 h. Tissue-specific expression profiling demonstrated that SfAntp was most abundantly expressed in the thoracic segments and legs. To functionally disrupt SfAntp, two sgRNA sites were designed at the first exon of SfAntp and the gene was knocked out by CRISPR/Cas9 via microinjection. The results showed that the deletion of SfAntp produced a mutant phenotype of thoracic fusion, thoracic leg defect, leg-like protrusions between the head and thoracic segments and pupation deformity. In addition, deletion of SfAntp resulted in high embryo mortality. Through DNA sequencing, it was found that the target site of the SfAntp mutant had different degrees of frameshift mutations, indicating that the mutant phenotype was indeed caused by the knockout of SfAntp.

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Section: Introductionmentioning

confidence: 99%

CRISPR/Cas9-Mediated Mutagenesis of Antennapedia in Spodoptera frugiperda

Wang,

Zhao,

Liu

et al. 2023

Insects

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The Hox protein Antennapedia orchestrates Drosophila adult flight muscle development

Poliacikova,

Aouane,

Caruso

et al. 2024

Sci. Adv.

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Muscle development and diversity require a large number of spatially and temporally regulated events controlled by transcription factors (TFs). Drosophila has long stood as a model to study myogenesis due to the highly conserved key TFs involved at all stages of muscle development. While many studies focused on the diversification of Drosophila larval musculature, how distinct adult muscle types are generated is much less characterized. Here, we identify an essential regulator of Drosophila thoracic flight muscle development, the Hox TF Antennapedia (Antp). Correcting a long-standing belief that flight muscle development occurs without the input of Hox TFs, we show that Antp intervenes at several stages of flight muscle development, from the establishment of the progenitor pool in the embryo to myoblast differentiation in the early pupa. Furthermore, the precisely regulated clearance of Hox in the developing flight muscle fibers is required to allow for fibrillar muscle fate diversification, setting these muscles apart from all other adult tubular muscle types.

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Novel Antennapedia and Ultrabithorax trimeric complexes with TBP and Exd regulate transcription

Villarreal-Puente,

Altamirano-Torres,

Jiménez-Mejía

et al. 2024

Hereditas

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Background Hox proteins interact with DNA and many other proteins, co-factors, transcriptional factors, chromatin remodeling components, non-coding RNAs and even the extracellular matrix that assembles the Hox complexes. The number of interacting partners continues to grow with diverse components and more transcriptional factors than initially thought. Hox complexes present many activities, but their molecular mechanisms to modulate their target genes remain unsolved. Results In this paper we showed the protein-protein interaction of Antp with Ubx through the homeodomain using BiFC in Drosophila. Analysis of Antp-deletional mutants showed that AntpHD helixes 1 and 2 are required for the interaction with Ubx. Also, we found a novel interaction of Ubx with TBP, in which the PolyQ domain of TBP is required for the interaction. Moreover, we also detected the formation of two new trimeric complexes of Antp with Ubx, TBP and Exd using BiFC-FRET; these proteins, however, do not form a trimeric interaction with BIP2 or TFIIEβ. The novel trimeric complexes reduced Antp transcriptional activity, indicating that they could confer specificity for repression. Conclusions Our results increase the number of transcriptional factors in the Antp and Ubx interactomes that form two novel trimeric complexes with TBP and Exd. We also report a new Ubx interaction with TBP. These novel interactions provide important clues of the dynamics of Hox-interacting complexes involved in transcriptional regulation, contributing to better understand Hox function.

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Antennapedia: The complexity of a master developmental transcription factor

Cited by 3 publications

References 108 publications

CRISPR/Cas9-Mediated Mutagenesis of Antennapedia in Spodoptera frugiperda

CRISPR/Cas9-Mediated Mutagenesis of Antennapedia in Spodoptera frugiperda

The Hox protein Antennapedia orchestrates Drosophila adult flight muscle development

Novel Antennapedia and Ultrabithorax trimeric complexes with TBP and Exd regulate transcription

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