Anterior and Posterior Optic Nerve Head Blood Flow in Nonhuman Primate Experimental Glaucoma Model Measured by Laser Speckle Imaging Technique and Microsphere Method

Wang, Lin; Cull, Grant; Piper, Chelsea; Burgoyne, Claude F.; Fortune, Brad

doi:10.1167/iovs.12-10911

Cited by 141 publications

(143 citation statements)

References 36 publications

Supporting

Mentioning

135

Contrasting

Order By: Relevance

“…Therefore, it is plausible that vascular insufficiency of the small vessels occurs in the prelaminar region of the glaucomatous optic disc. This finding might agree with previous reports on preperimetric glaucoma using laser speckle flowgraphy 13,17 and the microsphere method. 13 However, the discriminatory power of the PLFI/UA to differentiate eyes with POAG from normal eyes was weak compared to the VD (AUC 0.832, P ¼ 0.002, Table 5).…”

Section: Discussionsupporting

confidence: 94%

“…1 In experimental glaucoma, dysfunctions in autoregulatory mechanisms might lead to initial short-term increases and later decreases in the blood flow based on a laser speckle flow graphic study of the posterior optic nerve blood flow. 13 In this study, decreases in the VD in OH eyes occur in the superficial peripapillary retina and might reflect dysregulation of the blood flow in the superficial optic disc.…”

Section: Discussionmentioning

confidence: 61%

“…14 If the optimal interscan time to visualize small vessels was 1.5 to 3 ms, the interscan time of 5 ms in the AngioVue can lead to easy signal saturation from the large vessels. 13 If the signal from the large vessels is saturated and the caliber of the large vessels is relatively unchanged, the signals from the large vessels will not interfere with quantitative analysis of the flow index of the small vessels in the optic disc.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Discriminatory Power of Superficial Vessel Density and Prelaminar Vascular Flow Index in Eyes With Glaucoma and Ocular Hypertension and Normal Eyes

Chihara¹,

Dimitrova

Amano³

et al. 2017

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

PURPOSE. We evaluate the ability of optical coherence tomography angiography parameters, such as the peripapillary vessel density of the superficial retina and prelaminar flow index of the optic disc (PLFI), to differentiate primary open-angle glaucoma (POAG) and ocular hypertension (OH) from normal eyes. METHODS.The vessel density, PLFI, mean deviation of the visual field, circumpapillary retinal nerve fiber layer thickness (cpNFLT), and global loss volume of the ganglion cell complex were evaluated in one eye of 105 subjects with POAG and OH and normal eyes. The discriminatory powers of these parameters were evaluated based on the area under the curve (AUC) of the receiver operation characteristic curve and multiple comparisons.RESULTS. The vessel density (P < 0.001) and PLFI/unit area (PLFI/UA; P ¼ 0.020) in eyes with POAG were significantly less than in normal eyes. The vessel density in eyes with OH was significantly (P ¼ 0.018) reduced, whereas the PLFI/UA, global loss volume and cpNFLT were unaffected. The AUCs of the vessel density to discriminate glaucoma and OH from normal eyes were 0.832 and 0.724, respectively, and were significantly better than the PLFI/UA, in which the AUCs were 0.662 (P ¼ 0.002) and 0.569 (P ¼ 0.038), respectively. The powers of the vessel density and PLFI/UA to discriminate POAG from normal eyes were inferior to the global loss volume (P ¼ 0.006 and <0.0001) and cpNFLT (P ¼ 0.055 and P < 0.0001, respectively). CONCLUSIONS.The vessel density and PLFI/UA decreased significantly in glaucomatous eyes. The vessel density was more efficient than the PLFI/UA for differentiating glaucoma and OH from normal eyes.

show abstract

Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Discriminatory Power of Superficial Vessel Density and Prelaminar Vascular Flow Index in Eyes With Glaucoma and Ocular Hypertension and Normal Eyes

Chihara¹,

Dimitrova

Amano³

et al. 2017

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

show abstract

“…[52][53][54] It already has been used in various animal models previously to assess the validity of other blood flow measurement techniques, such as magnetic resonance (MR), 28 positron emission tomography (PET), 27 computed tomography (CT), 29 as well as ultrasound perfusion imaging techniques, 31 laser Doppler velocimetry, 26,30 and LSFG. 43,55 In this study, we found an excellent agreement of TRBF measurements assessed with dual-beam Doppler OCT and the microsphere method. The conformity of both approaches can be well appreciated in the Bland-Altman plot (see Fig.…”

Section: Discussionsupporting

confidence: 69%

Total Retinal Blood Flow in a Nonhuman Primate Optic Nerve Transection Model Using Dual-Beam Bidirectional Doppler FD-OCT and Microsphere Method

Told

Wang

Cull

et al. 2016

Invest. Ophthalmol. Vis. Sci.

Self Cite

View full text Add to dashboard Cite

Citation: Told R, Wang L , Cull G, et al. Total retinal blood flow in a nonhuman primate optic nerve transection model using dual-beam bidirectional Doppler FD-OCT and microsphere method. Invest Ophthalmol Vis Sci. 2016;57:143257: -144057: . DOI:10.1167 PURPOSE. We validated noninvasive Doppler-optical coherence tomography (OCT) blood flow measurements against the terminal microsphere method in a surgical induced optic nerve transection nonhuman primate model. METHODS.In 6 nonhuman primates, total retinal blood flow (TRBF) was measured with a custom-built dual-beam bidirectional Doppler Fourier Domain (FD)-OCT. Peripapillary retinal nerve fiber layer thickness (RNFLT) was measured by Spectralis spectral-domain (SD)-OCT. Measurements were performed every 10 to 15 days before and after unilateral optic nerve transection (ONT) until RNFLT was reduced by more than 40% from baseline. Before the animals were killed, TRBF was measured using the microsphere technique. RESULTS.A significant correlation between all arterial and venous Doppler OCT TRBF measurements was found in ONT and contralateral control eyes (both P < 0.01, n ¼ 6). The Bland-Altman analysis showed a bias of 0.57 in the ONT group and 0.02 in the contralateral control group. Also, excellent agreement was observed between Doppler OCT and microsphere measurements (P < 0.01, r ¼ 0.976, bias ¼ 0.54). After ONT, TRBF and RNFLT decreased by À51% 6 42% and À44% 6 2% (n ¼ 5), respectively. In the contralateral control eyes, TRBF and RNFLT were unchanged.CONCLUSIONS. Very good accordance was found between TRBF measurements, obtained with dual-beam bidirectional Doppler FD-OCT and the microsphere method. It also was possible to monitor changes over time in TRBF after ONT with Doppler OCT. These findings highlight the accuracy and potential of noninvasive Doppler OCT to provide valuable information for detecting early changes in ocular disease in future.

show abstract

“…Beyond bench-side physiological research, there are direct implications for prognostic, diagnostic, and intraoperative imaging applications, as speckle imaging of microcirculatory flows is increasingly becoming applied for gauging local and systemic tissue health [8,9]. Consequently, laser speckle flowmetry studies are expanding in dermatological [10][11][12][13][14], ophthalmological [15][16][17], and neurosurgical settings [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Optimization of camera exposure durations for multi-exposure speckle imaging of the microcirculation

Kazmi

Balial

Dunn

2014

Biomed. Opt. Express

View full text Add to dashboard Cite

Improved Laser Speckle Contrast Imaging (LSCI) blood flow analyses that incorporate inverse models of the underlying laser-tissue interaction have been used to develop more quantitative implementations of speckle flowmetry such as Multi-Exposure Speckle Imaging (MESI). In this paper, we determine the optimal camera exposure durations required for obtaining flow information with comparable accuracy with the prevailing MESI implementation utilized in recent in vivo rodent studies. A looping leave-one-out (LOO) algorithm was used to identify exposure subsets which were analyzed for accuracy against flows obtained from analysis with the original full exposure set over 9 animals comprising n = 314 regional flow measurements. From the 15 original exposures, 6 exposures were found using the LOO process to provide comparable accuracy, defined as being no more than 10% deviant, with the original flow measurements. The optimal subset of exposures provides a basis set of camera durations for speckle flowmetry studies of the microcirculation and confers a twofold faster acquisition rate and a 28% reduction in processing time without sacrificing accuracy. Additionally, the optimization process can be used to identify further reductions in the exposure subsets for tailoring imaging over less expansive flow distributions to enable even faster imaging.

show abstract

Anterior and Posterior Optic Nerve Head Blood Flow in Nonhuman Primate Experimental Glaucoma Model Measured by Laser Speckle Imaging Technique and Microsphere Method

Abstract: Chronic IOP elevation causes significant ONH BF decreases in the EG model. The high correlation between the BF reduction measured by LSFG and the microsphere method provides evidence that the LSFG is capable of assaying BF for a critical deep ONH region.

Cited by 141 publications

References 36 publications

Discriminatory Power of Superficial Vessel Density and Prelaminar Vascular Flow Index in Eyes With Glaucoma and Ocular Hypertension and Normal Eyes

Discriminatory Power of Superficial Vessel Density and Prelaminar Vascular Flow Index in Eyes With Glaucoma and Ocular Hypertension and Normal Eyes

Total Retinal Blood Flow in a Nonhuman Primate Optic Nerve Transection Model Using Dual-Beam Bidirectional Doppler FD-OCT and Microsphere Method

Optimization of camera exposure durations for multi-exposure speckle imaging of the microcirculation

Contact Info

Product

Resources

About