Anterior Pituitary Hormone in the Blood of Women With Ovarian Deficiency

Fluhmann, C. F.

doi:10.1001/jama.1929.02710090012005

Cited by 46 publications

(5 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The relative gonadotropic activity of whole plasma and of its various fractions is shown in Table I Fluhmann's observations ( 1 ) . Employing as his test animal the immature female mouse, which is approximately 6 times as sensitive to human menopausal gonadotropin as the rat ( 7 ) .…”

Section: Harvard Medical Schoolmentioning

confidence: 99%

Distribution and Partial Purification of Pituitary Gonadotropins of Human Plasma.

et al. 1956

Experimental Biology and Medicine

View full text Add to dashboard Cite

The presence of a substance or substances possessing gonadotropic activity, presumably of pituitary origin, in the serum of ovariectomized women was first demonstrated by Fluhmann( 1 ) . Subsequently, Cohn and his collaborators ( 2 ) reported the detection of two gonadotropic hormones in fractions prepared from certain pools of human plasma. Since these findings could not be fully confirmed in later studies,t we have reexamined the distribution of pituitary gonadotropic activity among various plasma fractions. The bulk of the gonadotropic activity has been located in Fraction I1 + 111, and by subfractionation has been partially purified. Preliminary reports of these studies have appeared elsewhere (394) 9Methods and materials. Postmenopausal plasma was employed exclusively in this study, preliminary bioassays having shown that gonadotropic activity is readily demonstrable in the whole plasma of postmenopausal women, but not in that of men or of adult women of menstrual age. The donors were healthy women 65 years of age or less who were not undergoing treatment with estrogenic preparations of any kind.$ The blood was collected either with citrate or through cationic exchange resins in the ADL-Cohn blood fractionating machine. The data * This work was supported by grants from Barbara C. Willcox Fund of Vincent Memorial Hospital, Amer. Cancer SOC. (Mass. Division), and 'by research grant RG-4444 from National I.H., P.H.S. t Unpulblished data.$ Previous experience has shown that unwitting ingestion of estrogen occasionally results in significant contamination of plasma !pools. Therefore, the whole plasma and those fractions found to produce an increase in uterine weight of intact rats were assayed for estrogen in spayed rats by (modification of method of Bulbring and Burn(5). No indication of estrogenic activity was found. Harvard Medical Schoolherein reported were obtained by fractionating a 10-liter plasma pool which comprised the donations of 40 women, and are identical with the results previously obtained from the fractionation of 20 individual or pooled plasma samples from 3-12 donors. The cold ethanol method VI described by Cohn et al.(6) was employed for the initial plasma fractionation. The following fractions were prepared: Fraction I containing the fibrinogen; I1 + I11 consisting of P-lipoproteins and gamma globulin ; IV-1 containing the a-globulins and cholesterol; IV-4 consisting of Uand P-globulins and the metal-combining globulin; V, which contains the bulk of the albumin ; and VI, the supernatant remaining after fractionation. Partial purification of the gonadotropically-active material in Fraction I1 + I11 was accomplished by precipitating the bulk of the biologically-inactive proteins with zinc. Fraction I1 + I11 was brought to the original plasma volume with 0.15 M sodium chloride and zinc glycinate added until a 5 mM zinc concentration was obtained at a pH of 6.8 and a temperature of 0°C. The supernatant was freed of zinc by dialysis against ethylene diamine tetra-acetic acid (EDTA) and sodium citr...

show abstract

Section: Harvard Medical Schoolmentioning

confidence: 99%

Distribution and Partial Purification of Pituitary Gonadotropins of Human Plasma.

et al. 1956

Experimental Biology and Medicine

View full text Add to dashboard Cite

show abstract

“…Meanwhile, however, attention had been turned to the possibility of detecting similar active substances in conditions other than that of pregnancy. Fluhmann (23) …”

Section: Fractionation Of the Neutral Fraction From Urinementioning

confidence: 99%

Somatic and Endocrine Studies of Puberal and Adolescent Boys

Greulich¹

1942

Monographs of the Society for Research in Child Development

View full text Add to dashboard Cite

“…Central to the reproductive system are cells within the anterior pituitary gland, which secrete two so-called “gonadotropins”—luteinizing hormone (LH) and follicle-stimulating hormone (FSH)—which control both gametogenesis and hormone production in the testes and ovary. Although our recognition of the role played by LH and FSH in relation to gonadal function dates back to the 1920s (Fluhmann, 1929), it was midcentury before the possibility that the hypothalamus might serve to regulate pituitary function, including that of the gonadotropin-secreting cells, was put forward by Green and Harris (1947). As depicted in Fig.…”

Section: Introductionmentioning

confidence: 99%

Chapter 14 Biomathematical Modeling of Pulsatile Hormone Secretion

Evans

Farhy

Johnson

2009

Methods in Enzymology

View full text Add to dashboard Cite

Shortly after the recognition of the profound physiological significance of the pulsatile nature of hormone secretion, computer-based modeling techniques were introduced for the identification and characterization of such pulses. Whereas these earlier approaches defined perturbations in hormone concentration-time series, deconvolution procedures were subsequently employed to separate such pulses into their secretion event and clearance components. Stochastic differential equation modeling was also used to define basal and pulsatile hormone secretion. To assess the regulation of individual components within a hormone network, a method that quantitated approximate entropy within hormone concentration-times series was described. To define relationships within coupled hormone systems, methods including cross-correlation and cross-approximate entropy were utilized. To address some of the inherent limitations of these methods, modeling techniques with which to appraise the strength of feedback signaling between and among hormone-secreting components of a network have been developed. Techniques such as dynamic modeling have been utilized to reconstruct dose-response interactions between hormones within coupled systems. A logical extension of these advances will require the development of mathematical methods with which to approximate endocrine networks exhibiting multiple feedback interactions and subsequently reconstruct their parameters based on experimental data for the purpose of testing regulatory hypotheses and estimating alterations in hormone release control mechanisms.

show abstract

Anterior Pituitary Hormone in the Blood of Women With Ovarian Deficiency

Cited by 46 publications

References 12 publications

Distribution and Partial Purification of Pituitary Gonadotropins of Human Plasma.

Distribution and Partial Purification of Pituitary Gonadotropins of Human Plasma.

Somatic and Endocrine Studies of Puberal and Adolescent Boys

Chapter 14 Biomathematical Modeling of Pulsatile Hormone Secretion

Contact Info

Product

Resources

About