Anthelmintic activity of Piper sylvaticum Roxb. (family: Piperaceae): In vitro and in silico studies

Paul, Arkajyoti; Adnan, Md.; Majumder, Mohuya; Kar, Niloy; Meem, Muntasir; Rahman, Mohammed Shahariar; Rauniyar, Akash Kumar; Rahman, Nishat; Chy, Md. Nazim Uddin; Kabir, Mohammad Shah Hafez

doi:10.1186/s40816-018-0077-8

Cited by 23 publications

(16 citation statements)

References 19 publications

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“…Again, it gives us not only the possible mechanism of action of a protein or enzyme but also the binding moods inside the binding site of proteins or enzyme. So, we have also used the molecular docking of some compounds of P. chaba to make a collaboration between this compounds and different types of enzymes responsible for inflammation to illustrate the biochemical process of the antiinflammatory activity [32][33][34]. These compounds were docked against three targets which were COX-1 (PDB ID: 2OYE), COX-2 (PDB ID: 6COX) and NF-κβ (PDB ID: 5LDE).…”

Section: Discussionmentioning

confidence: 99%

Membrane stabilization as a mechanism of the anti-inflammatory activity of ethanolic root extract of Choi (Piper chaba)

et al. 2020

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Background: This experiment is conducted to evaluate the anti-inflammatory effect of Piper chaba roots. Methods: The in-vitro anti-inflammatory activity of Piper chaba was carried out by human red blood cell (HRBC) membrane stabilization method which includes heat-induced hemolysis and hypo tonicity-induced hemolysis and also by another method of egg albumin denaturation assay. Results: Anti-inflammatory activity study of crude ethanolic extract was performed using heat induced membrane stabilization method, hypo-tonicity induced HRBC membrane stabilization method and egg albumin denaturation method. Crude ethanolic extracts of P. chaba showed promising in vitro anti-inflammatory activity in a concentration dependent manner. Using acetyl salicylic acid (ASA) as standard drug and was compared with ethanolic extract to determine anti-inflammatory activity. Heat induced anti-inflammatory test revealed that crude ethanolic extract of P. chaba (500 μg/ml) and positive control ASA(500 μg/ml) have 52.667% and 78% respectively, hypo tonicity induced antiinflammatory test showed 35.67% and 59% inhibition of red blood cell (RBC) hemolysis. Egg albumin denaturation method also evaluated that crude ethanolic extract (1000 μg/ml) and ASA (1000 μg/ml) showed 60% and 97.12% inhibition of egg albumin denaturation. Conclusion: The plant of P. chaba of the genus Piper possesses promising anti-inflammatory activities.

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Section: Discussionmentioning

confidence: 99%

Membrane stabilization as a mechanism of the anti-inflammatory activity of ethanolic root extract of Choi (Piper chaba)

et al. 2020

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“…A cubic box of specific dimensions centered on the centroid of the active site residues was generated for the receptor. The bounding box was set to 14 Å × 14 Å × 10 Å and it's essential to identify the active binding site in the target protein [29].…”

Section: Grid Generationmentioning

confidence: 99%

In vivo and in silico evaluation of analgesic activity of Lippia alba

Aziz¹,

Mehedi

Akter

et al. 2019

Clin Phytosci

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Background: This study was conducted to evaluate the analgesic activity of different extracts of Lippia alba (L. alba) along with in silico evaluation of analgesic activity of the isolated compounds from L. alba against cyclooxygenase-2 enzyme and ADME/T analysis of isolated compounds. Method: In vivo analgesic activity of different extracts of L. alba was evaluated by acetic acid-induced writhing, tail immersion and hot plate on Swiss albino mice of either sex. In silico activity of the isolated compounds and ADME/T analysis were performed by Schrödinger-Maestro (Version 10.1) and OSIRIS Data warrior (version 4.6.1) softwares. Results: Three different extracts (Methanolic extract: ME; Petroleum ether extract: PEE; Dichloromethane extract: DCME) of 250 mg/kg and 500 mg/kg doses were used in the experiments to evaluate analgesic activity. In acetic acid-induced writhing test, significant results were seen for PEE (500 mg/kg) and DCME (500 mg/kg), which were 53.09 ± 2.87 & 50.09 ± 4.24%, respectively. In tail immersion test, the best latency time was found at + 60 min for PEE (500 mg/kg) which is (5.65 ± 0.25) sec. For hot plate test, DCME at a dose 500 mg/kg showed the highest increase in latency time, which was 13.48 ± 0.33 s. In the case of in silico evaluation of analgesic activity, the compounds such as geranial, neral, (E)caryophyllene, caryophyllene oxide, mussaenide, and 8-epi-loganin meet the condition of Lipinski's rule of five. Among these safe compounds, 8-epi-loganin showed the best docking score of − 8.17 kcal/mol against cyclooxygenase-2 enzyme (PDB ID: 6COX), which was almost similar to that of the standard drug, Celecoxib (− 11.11 kcal/mol). Conclusion: In conclusion, L. alba can be a potent source of analgesic medicine and further modification and simulation studies are required to establish the effectiveness of 8-epi-loganin.

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“…Additionally, a quantitative phytochemical analysis of MEPSL also reported that it contains substantial amounts of phenol (65.83 mg gallic acid equivalent/g dried extract), flavonoids (102.56 mg quercetin equivalent/g dried extract), and condensed tannins (89.32 mg catechin equivalent/g dried extract). Furthermore, MEPSS contains significant amounts of phenol (93.39 mg), flavonoids (53.74 mg), and condensed tannins (55.82 mg) [9,11,14]. On the other hand, a previous acute toxicity study described that the plant had no mortality, abnormal behavior, and neurological changes up to 2000 mg/kg dose, which is a clear indication that the plant extract has low toxicity profile and is safe for a therapeutic dose [11].…”

Section: Resultsmentioning

confidence: 97%

“…Kumar et al reported antioxidant activity of the roots and fruits [13]. Paul et al reported the anthelmintic activity of stem [14] and Haque et al described the antidiarrheal activity of the stem [15]. Chy et al stated that the plant (stem) has anti-nociceptive and anti-inflammatory properties [11].…”

Section: Introductionmentioning

confidence: 99%

Comparative Study of Piper sylvaticum Roxb. Leaves and Stems for Anxiolytic and Antioxidant Properties Through In Vivo, In Vitro, and In Silico Approaches

et al. 2020

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Piper sylvaticum Roxb. is traditionally used by the indigenous people of tropical and subtropical countries like Bangladesh, India, and China for relieving the common cold or a variety of chronic diseases, such as asthma, chronic coughing, piles, rheumatic pain, headaches, wounds, tuberculosis, indigestion, and dyspepsia. This study tested anxiolytic and antioxidant activities by in vivo, in vitro, and in silico experiments for the metabolites extracted (methanol) from the leaves and stems of P. sylvaticum (MEPSL and MEPSS). During the anxiolytic evaluation analyzed by elevated plus maze and hole board tests, MEPSL and MEPSS (200 and 400 mg/kg, body weight) exhibited a significant and dose-dependent reduction of anxiety-like behavior in mice. Similarly, mice treated with MEPSL and MEPSS demonstrated dose-dependent increases in locomotion and CNS simulative effects in open field test. In addition, both extracts (MEPSL and MEPSS) also showed moderate antioxidant activities in DPPH scavenging and ferric reducing power assays compared to the standard, ascorbic acid. In parallel, previously isolated bioactive compounds from this plant were documented and subjected to a molecular docking study to correlate them with the pharmacological outcomes. The selected four major phytocompounds displayed favorable binding affinities to potassium channel and xanthine oxidoreductase enzyme targets in molecular docking experiments. Overall, P. sylvaticum is bioactive, as is evident through experimental and computational analysis. Further experiments are necessary to evaluate purified novel compounds for the clinical evaluation.

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Anthelmintic activity of Piper sylvaticum Roxb. (family: Piperaceae): In vitro and in silico studies

Cited by 23 publications

References 19 publications

Membrane stabilization as a mechanism of the anti-inflammatory activity of ethanolic root extract of Choi (Piper chaba)

Membrane stabilization as a mechanism of the anti-inflammatory activity of ethanolic root extract of Choi (Piper chaba)

In vivo and in silico evaluation of analgesic activity of Lippia alba

Comparative Study of Piper sylvaticum Roxb. Leaves and Stems for Anxiolytic and Antioxidant Properties Through In Vivo, In Vitro, and In Silico Approaches

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