Anthelmintic and in vitro antioxidant evaluation of fractions of methanol extract of Leea asiatica leaves

Abstract

Leea asiatica, a folk medicinal plant of India, is used in the treatment of worm infection and other oxidative stress-related disorders, traditionally. In the present study, the in vitro anthelmintic and in vitro antioxidant activity of different fractions of the methanol extract from the Leea asiatica leaves were evaluated. The fraction displayed significant anthelmintic activity against Indian adult earthworms (Pheretima posthuma). The ethyl acetate fraction showed a better paralysis activity (13.99 ± 0.59), while the methanol fraction showed a better death time (63.76 ± 0.73 minutes), when compared with other fractions, at a dose of 50 mg/ml concentration. The anthelmintic activity of methanol and the ethyl acetate fraction were almost similar and comparable to the standard drug, piperazine citrate. The petroleum ether fraction did not produce a potent anthelmintic effect compared to the standard. The in vitro antioxidant activity was evaluated by using the diphenyl-picrylhydrazyl (DPPH) radical scavenging assay, nitric oxide radical scavenging assay, lipid peroxidation assay, and the ferric thiocyanate method. The ethyl acetate fraction showed better antioxidant activity in all tested methods. The IC50 value of the ethyl acetate fraction in the DPPH radical, nitric oxide radical scavenging assay, and lipid peroxidation assay were 9.5, 13.0, and 57.0 μg/ml, respectively. The fractions significantly (P < 0.05) inhibited the peroxidation of linoleic acid. The results confirmed the folk use of Leea asiatica in warm infection and the plant could be viewed as a potential source of natural anthelmintic and antioxidant compound.

Phytochemical Study of Aerial Parts of Leea asiatica

“…Leea asiatica has also been used as a folk medicine, especially in India, to treat worm infection, bone fracture, liver disorder, and oxidative stress-related diseases. Up to date, three published reports have described the biological effects of L. asiatica extracts, including anthelmintic and antioxidant-related nephroprotective and hepatoprotective activities [14,15,16], and no phytochemical study on this species has been attempted yet even though L. asiatica has been used as a traditional medicine, as have other Leea species. The identification of the phytochemicals of herbal medicine is very important because it can be used as a primary data for the prediction of biological effects, safety information, and clarification of medicinal use.…”

Hepatoprotective and antioxidant activity of Leea asiatica leaves against acetaminophen-induced hepatotoxicity in rats

“…We have conducted a ethnomedicinal survey in Tripura, India and found that the plant is used to treat liver disorder by the ethnic people (Bhandary et al, 1995;Prasad et al, 2008;. In our previous investigation we reported that leaves of L. asiatica possess in vitro antioxidant, anthelmintic and nephroprotective activity (Sen et al, , 2012 (Manokaran et al, 2008;Singh et al, 1995). After 48 h of acetaminophen administration, the blood was collected under light ether anaesthesia and serum was separated by centrifugation of blood at 4000 × g, which was used for the biochemical estimations.…”

Anthelmintic Activity of Sub-Fractions From the N-Hexane Fraction of Picria Fel-Terrae Leaves on Pheretima Posthuma

“…However, the earthworms have been widely used for the initial study of in vitro anthelmintic activity [10]. According to Sen et al, substances that have anthelmintic activity on the earthworms is also effective against parasitic worms of human [11]. The present study exhibited the anthelmintic activity of SF4 is most potent to P. posthuma among the others even than the standard pyrantel.…”