Anthelmintic drugs and nematicides: studies in Caenorhabditis elegans

Holden‐Dye, Lindy; Walker, Robert

doi:10.1895/wormbook.1.143.2

Cited by 136 publications

(152 citation statements)

References 200 publications

Supporting

Mentioning

138

Contrasting

Unclassified

Order By: Relevance

“…Nematicides are used to control plant parasite nematodes, which are ubiquitous and globally cause costly yield loses in agriculture [32,69]. To date, there have been limited studies demonstrating nematicides use on non-plant nematodes, as they are mostly treated by anthelminthic drugs.…”

Section: Nematicidesmentioning

confidence: 99%

“…However, fluensulfone has shown to be highly effective against a number of plant nematodes [18,74]. In their study, Kearn et al (2014) have studied fluensulfone effect on C. elegans , a genetic nematode model to study effects of different anthelminthic drugs and nematicides that are used against animal and human parasites [32]. It has been shown that a slightly higher dose of fluensulfone is required to have a similar effect on C. elegans as on plant parasite nematodes, inhibiting egg laying, hatching, development, feeding and moving stages of the nematode [74].…”

Section: Nematicidesmentioning

confidence: 99%

“…Most cholinesterase inhibiting nematicides have been banned or restricted for use due to their adverse toxic effects on non-target organisms including humans, and the environment, which is associated with absence of species’ selectivity [32,71]. Another disadvantage of non-fumigant nematicides is their mobility in soil which can potentially cause widespread non-target toxic effects.…”

Section: Nematicidesmentioning

confidence: 99%

See 2 more Smart Citations

Advocating for both Environmental and Clinical Approaches to Control Human Strongyloidiasis

2016

View full text Add to dashboard Cite

Strongyloidiasis is an underestimated disease caused by the soil-transmitted parasite of the genus Strongyloides. It is prevalent in socioeconomically disadvantaged communities and it is estimated that global infection could be as high as 370 million people. This paper explores current methods of strongyloidiasis treatment, which rely on administration of anthelminthic drugs. However these drugs cannot prevent reinfection and drug resistance has already been observed in veterinary models. This highlights the need for a combined approach for controlling Strongyloides that includes both clinical treatment and environmental control methods. Currently, nematicides are widely used to control plant parasites. The review suggests that due to the species’ similarity and similar modes of action, these nematicides could also be used to control animal and human parasitic nematodes in the environment.

show abstract

Section: Nematicidesmentioning

confidence: 99%

Section: Nematicidesmentioning

confidence: 99%

Section: Nematicidesmentioning

confidence: 99%

See 1 more Smart Citation

Advocating for both Environmental and Clinical Approaches to Control Human Strongyloidiasis

2016

View full text Add to dashboard Cite

show abstract

“…According to latest estimates, more than 2 billion people are infected with these parasites [4]. Although many synthetic drugs including preparation, benzimidazole, imidazothiazole, and albendazole are available in the market, and in addition to it, various herbal plant parts also utilized as an anthelmintic agent, but these synthetic drugs are losing their effectiveness as resistance develops in nematodes against drugs [5]. Only a few studies have been conducted regarding the use of bacterial metabolites as anthelmintic [6][7][8], therefore in the present study, two probiotic strains: E. faecium BM10 KY788342 and Lactobacillus casei GM10 KY794586 evaluated for anthelmintic activity and antiinflammatory activity.…”

Section: Introductionmentioning

confidence: 99%

The Unexplored Roles of Probiotic Bacteria: In Vitro Anti-Inflammatory and Anthelmintic Activity of Enterococcus Faecium Bm10 Ky788342 and Lactobacillus Casei Gm10 Ky794586

Jain

Mehta

2017

Asian J Pharm Clin Res

View full text Add to dashboard Cite

Objective: The aim of the present study was to evaluate the in vitro anti-inflammation activity and anthelmintic potential of two novel isolated probiotic strains through Enterococcus faecium BM10 KY788342 and Lactobacillus casei GM10 KY794586. Methods:In vitro anti-inflammatory activity was evaluated using protein denaturation inhibition method. Pheretima posthuma was used as a suitable in vitro model, and time of paralysis and death were used as parameters to evaluate anthelmintic potential of probiotic strains.Results: Lyophilized solutions of L. casei GM10 showed significant protein denaturation inhibition (56.20±0.86%) followed by E. faecium BM10 (52.28±0.31%) comparable to diclofenac (93.62±1.39%) at the maximum concentration of 250 μg/ml. Intracellular cell-free extract of E. faecium BM10 showed a strong anthelmintic (vermicidal) activity (6±0.23 minutes), followed by L. casei GM10 (9±0.05 minutes) comparable to piperazine citrate (20±0.422 minutes) and albendazole (24±0.43 minutes) at the maximum concentration of 100 mg/ml. Conclusion:Results of the present study concluded that both tested lactic acid bacteria strains exhibited significant in vitro anti-inflammatory activity and can be used as potent and safe anthelmintic agent.

show abstract

“…These phenotypes are easily scored but provide little insight into underlying mechanisms. For high-throughput screening, the free-living nematode Caenorhabditis elegans offers convenience, low cost and molecular-genetic tools (Holden-Dye and Walker, 2014, Burns et al., 2015), but has yet to produce a commercial product (Geary et al., 2015). Hits identified in C. elegans must subsequently be tested on the targeted parasitic species or close relatives.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic platform for electrophysiological recordings from host-stage hookworm and Ascaris suum larvae: A new tool for anthelmintic research

Weeks

Roberts

Robinson

et al. 2016

International Journal for Parasitology: Drugs and Drug Resistan

View full text Add to dashboard Cite

The screening of candidate compounds and natural products for anthelmintic activity is important for discovering new drugs against human and animal parasites. We previously validated in Caenorhabditis elegans a microfluidic device (‘chip’) that records non-invasively the tiny electrophysiological signals generated by rhythmic contraction (pumping) of the worm's pharynx. These electropharyngeograms (EPGs) are recorded simultaneously from multiple worms per chip, providing a medium-throughput readout of muscular and neural activity that is especially useful for compounds targeting neurotransmitter receptors and ion channels. Microfluidic technologies have transformed C. elegans research and the goal of the current study was to validate hookworm and Ascaris suum host-stage larvae in the microfluidic EPG platform. Ancylostoma ceylanicum and A. caninum infective L3s (iL3s) that had been activated in vitro generally produced erratic EPG activity under the conditions tested. In contrast, A. ceylanicum L4s recovered from hamsters exhibited robust, sustained EPG activity, consisting of three waveforms: (1) conventional pumps as seen in other nematodes; (2) rapid voltage deflections, associated with irregular contractions of the esophagus and openings of the esophogeal-intestinal valve (termed a ‘flutter’); and (3) hybrid waveforms, which we classified as pumps. For data analysis, pumps and flutters were combined and termed EPG ‘events.’ EPG waveform identification and analysis were performed semi-automatically using custom-designed software. The neuromodulator serotonin (5-hydroxytryptamine; 5HT) increased EPG event frequency in A. ceylanicum L4s at an optimal concentration of 0.5 mM. The anthelmintic drug ivermectin (IVM) inhibited EPG activity in a concentration-dependent manner. EPGs from A. suum L3s recovered from pig lungs exhibited robust pharyngeal pumping in 1 mM 5HT, which was inhibited by IVM. These experiments validate the use of A. ceylanicum L4s and A. suum L3s with the microfluidic EPG platform, providing a new tool for screening anthelmintic candidates or investigating parasitic nematode feeding behavior.

show abstract

Anthelmintic drugs and nematicides: studies in Caenorhabditis elegans

Cited by 136 publications

References 200 publications

Advocating for both Environmental and Clinical Approaches to Control Human Strongyloidiasis

Advocating for both Environmental and Clinical Approaches to Control Human Strongyloidiasis

The Unexplored Roles of Probiotic Bacteria: In Vitro Anti-Inflammatory and Anthelmintic Activity of Enterococcus Faecium Bm10 Ky788342 and Lactobacillus Casei Gm10 Ky794586

Microfluidic platform for electrophysiological recordings from host-stage hookworm and Ascaris suum larvae: A new tool for anthelmintic research

Contact Info

Product

Resources

About