Anthelmintic effect of a methanol extract of leaves of Dregea volubilis on Paramphistomum explanatum

Abstract

Dregea volubilis (family Asclepediaceae) is widely used as anthelmintic in traditional system of medicine in eastern and southern part of India. The aim of this study was to evaluate the anthelmintic activity of the methanol extract of D. volubilis leaves (MEDV) and to observe its effect through SEM study. Live parasites (trematode Paramphistomum explanatum) were collected from buffalo in 0.9% phosphate-buffered saline (PBS). It was incubated at 37 ± 1°C in media containing either no extract (control), the test drug, MEDV at four dose levels (10, 25, 50, and 100 mg/ml) or the standard drug, albendazole, at a dose of 10 mg/ml. The effectiveness of the extract was judged on the basis of the loss of spontaneous movement and/or complete destruction or death of the trematodes. After being removed from the experimental medium, trematodes were dipped in PBS at 37 ± 1°C and on gentle stimulation, the paralyzed parasite showed immobility. Death was confirmed when it completely lost its motility even when vigorously shaken or dipped in warm water (50°C). The trematodes, both drug treated and others, were further processed for SEM study using standard method. The anthelmintic activity was found with all the doses through paralysis and death of the organisms (p < 0.001). Maximum anthelmintic activity was found with a dose of 100 mg/ml. Minor damage was observed with both suckers, but severe distortion was found with tegumental surface of the treated trematodes. The present SEM-based study established the anthelmintic activity of MEDV.

Gastrothylax crumenifer: Ultrastructure and histopathology study of in vitro trematodicidal action of Marattia fraxinea (Sm.)

“…Various studies on in vitro and in vivo anti-trematodal activity of medicinal plants viz., alcoholic extract of Allium sativum and Piper longum [58], Balanites aegyptica [17], bark of Prosopis cineraria [18], Plumbagin on Fasciola gigantica and Paramphistomum cervi [37,59], 50 medicinal plants against Schistosoma mansoni and Echinostoma caproni used in Côte d' Ivoire [16], Bombax malabaricum leaves against Paramphistomum explanatum [15], Dregea volubilis leaves against Paramphistomum explanatum [60], Artemisia annua, A.absinthium, A.siminatriloba and Fumaria officinalis against adult Schistosoma mansoni, Fasciola hepatica and Echinostoma caproni in vitro [14], Aegle marmelos, Andrographis lineata, A.paniculata, Cocculuc hirsutus, Eclipta prostrata and Tagetes erecta against Paramphistomum cervi [13], Flemingia estita root tuber against Paramphistomum sp. [9].…”

Anthelmintic effect of a methanol extract of Bombax malabaricum leaves on Paramphistomum explanatum

“…A number of drugs have been assessed and recommended for the treatment of paramphistomosis (Panyarachun et al 2010;Rolfe and Boray 1988). It was reported earlier by our group that the methanol extract of leaves of Dregea volubilis was effective against P. explanatum (Hossain et al 2011b). Interestingly enough, it was found that different plants do possess anthelmintic activity (Agaie and Onyeyili 2007;Aswar et al 2008;Ferraz et al 2010;Mehlhorn et al 2011;Roy and Swargiary 2009;Schmahl et al 2010;Tandon et al 1997;Yadav and Tangpu 2009).…”

Screening of 42 medicinal plants for in vivo anthelmintic activity against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus)

“…Several studies proved that different plant extracts have significant killing effects in vitro and in vivo on nematodes, cestodes, and trematodes Klimpel et al 2011;Abdel-Ghaffar et al 2011). Hossain et al (2011 evaluated the anthelmintic activity of the methanol extract of Dregea volubilis leaves against Paramphistomum explanatum and observed its effect through SEM study.…”