Anthelmintic efficacy of Flemingia vestita (Fabaceae): effect of genistein on glycogen metabolism in the cestode, Raillietina echinobothrida

Tandon, Veena; Das, Bidyadhar; Saha, Nirmalendu

doi:10.1016/s1383-5769(03)00006-0

Cited by 26 publications

(26 citation statements)

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“…In addition, in vitro treatment of R. echinobothrida with the crude root-peel extract and genistein also caused alterations in the levels of the free amino acid pool and tissue ammonia (Tandon et al 1998). In an earlier study, we also showed that glycogen was decreased significantly due to increased glycogenolysis and decreased glycogenesis during treatments of R. echinobothrida with these plant materials (Tandon et al 2003).…”

Section: Introductionsupporting

confidence: 54%

“…In an earlier study, it was found that at the time of paralysis, the glycogen concentration in R. echinobothrida decreased by 19-44% under the influence of F. vestita derived components. This was accompanied by the stimulation of glycogenolytic enzymes, particularly the active form of glycogen phosphorylase (Tandon et al 2003). Glycolysis, the main energy-generating pathway, with lactate as an end product in nematode parasites (Omar et al 1996), seems to be a promising target for new drugs against trypanosomatid parasites, because this pathway plays an essential role in ATP supply (Lakhdar-Ghazal et al 2002).…”

Section: Discussionmentioning

confidence: 99%

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Anthelmintic efficacy of Flemingia vestita (Fabaceae): alteration in the activities of some glycolytic enzymes in the cestode, Raillietina echinobothrida

2004

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The crude root-peel extract of Flemingia vestita, genistein and praziquantel were tested against some selected glycolytic enzymes--hexokinase (HK), phosphofructokinase (PFK), phosphoenolpyruvate carboxykinase (PEPCK), pyruvate kinase (PK), lactate dehydrogenase (LDH), malate dehydrogenase (MDH) and malic enzyme (ME)--of the fowl tape worm, Raillietina echinobothrida. Following exposure to the various treatments, the activities of HK, PFK, PEPCK and LDH increased by 33-39%, 41-125%, 44-49% and 55-67%, respectively, and that of PK decreased by 14-26% in the parasite at the time of paralysis. The MDH and ME activities of the tissue homogenate were also found to be higher by 22-43% and 28-59%, respectively, in the treatments. However, whereas the activity of both cytosolic and mitochondrial MDH increased by 33-58% and 43-73%, respectively, the cytosolic ME activity showed an increase of 33-39%, and there was no significant enhancement in the mitochondrial ME activity. Histochemically, the enhancement in the activities of HK, LDH and MDH was clearly discernible. The enhanced glycolytic activity seems to be a function of anthelmintic stress caused by the phytochemicals.

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Section: Introductionsupporting

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Anthelmintic efficacy of Flemingia vestita (Fabaceae): alteration in the activities of some glycolytic enzymes in the cestode, Raillietina echinobothrida

2004

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“…Glucose and other simple carbohydrates are the main energy source for cestodes and trematodes (Roberts 1983;Bryant and Behm 1989). In an earlier study pertaining to the efficacy of Flemingia vestita-derived components, it was found that at the paralysis time, the glycogen concentration in Raillietina (Tandon et al 2003). The root peel extracts alterated the enzyme activity in the cestode and trematode tegument (Pal and Tandon 1998;Kar et al 2002).…”

Section: Discussionmentioning

confidence: 96%

Anthelmintic activity of ginger, curcumin, and praziquentel against Raillietina cesticillus (in vitro and in vivo)

Elbahy

Bazh

2015

Parasitol Res

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This work evaluates the anthelminitic activity of ginger and curcumin on the cestode Raillietina cesticillus. Live parasites were collected from intestine of naturally infected chickens in PBS 0.9 % and then incubated at 37 °C in media containing ginger extract at three different concentrations (125, 250, and 500 mg); every concentration was dissolved in 10 ml media. The curcumin extract was used at three different concentrations (250, 500, and 1000 mg); each was dissolved in 10 ml media. Praziquantel at a concentration of 600 mg was added to 10 ml media. A control one without extract was reported. Regression of worms increased gradually in all concentrations. At 500 mg ginger (50 ± 0 %), worms were regressed at 48 h post-exposure (h.p.e.). Also (50 ± 1.8 %), worms were regressed at 1000 mg curcumin at the same time. On the other hand, praziquantel showed the highest regression (65 ± 2.3 %). The extract efficacy was exhibit as concentration-time-dependent mainly at higher concentrations used after 48 h. In vivo effects of ginger and curcumin were lower than those in vitro.

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“…A remarkable level of increase of neurotoxic substances like ammonia and neuromodulatory amino acids namely g-amino-butyric acids and citrulline were observed in Fasciolopsis buski, an intestinal trematode, when treated with genistein (Kar et al, 2004). Further, the chemical is also found to be responsible for alteration of several enzymes of glucose and glycogen metabolism in R. echinobothrida (Das et al, 2004a,b;Tandon and Das, 2007;Tandon et al, 2003). Because genistein is also reported to be present in M. pachycarpa (Okamoto et al, 2006), probably the chemical suppresses tegumental enzyme activities in R. echinobothrida in a similar way leading to deformation and disruption of its tegument.…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural observations on tegumental surface of Raillietina echinobothrida and its alterations caused by root‐peel extract of Millettia pachycarpa

Roy

Dasgupta

Tandon

2008

Microscopy Res & Technique

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Millettia pachycarpa Benth (Leguminosae) has a usage in traditional medicine system practiced among the Lushai tribes of Mizoram, a state in North East India, who customarily consume the aqueous extract of the root peel of the plant to get rid of intestinal worm infections. The crude ethanol, methanol, and acetone fractions of the plant were assayed against Raillietina echinobothrida, the intestinal cestode parasite of domestic fowl, to authenticate the putative anthelmintic efficacy and cestocidal potential in particular of the plants. In vitro exposure of the worm to the extract at a concentration of 25 mg/mL phosphate buffered saline (at 37 degrees C +/- 1 degrees C) revealed distortion and disruption of mitochondria, nucleus, nucleolus, nuclear membrane, basal lamina, and tegumental vacuolization in the distal cytoplasm leading to scar formation in the surface. The possible use of the plant as a potential anthelmintic against cestode parasite is discussed.

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Anthelmintic efficacy of Flemingia vestita (Fabaceae): effect of genistein on glycogen metabolism in the cestode, Raillietina echinobothrida

Cited by 26 publications

References 14 publications

Anthelmintic efficacy of Flemingia vestita (Fabaceae): alteration in the activities of some glycolytic enzymes in the cestode, Raillietina echinobothrida

Anthelmintic efficacy of Flemingia vestita (Fabaceae): alteration in the activities of some glycolytic enzymes in the cestode, Raillietina echinobothrida

Anthelmintic activity of ginger, curcumin, and praziquentel against Raillietina cesticillus (in vitro and in vivo)

Ultrastructural observations on tegumental surface of Raillietina echinobothrida and its alterations caused by root‐peel extract of Millettia pachycarpa

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