Anthelmintic induced congenital malformations in sheep embryos using netobimin

Navarro, Marc; Cristòfol, Carles; Carretero, Ana; Arboix, M.; Ruberte, Jesús

doi:10.1136/vr.142.4.86

Cited by 31 publications

(19 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Different studies have shown teratogenic effects of the anthelmintic benzimidazoles Cristofol et al, 1995;Navarro et al, 1998;Teruel et al). The teratogenic activity of ABZ in vivo is attributed to its active albendazole sulphoxide (ABZSO) metabolite which has the capacity of reaching the embryos in elevated concentrations (Cristofol et al, 1995(Cristofol et al, , 1997Capece et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…Among the anthelmintic agents, benzimidazoles present a broad spectrum of activity with high effectiveness and safety (Campbell, 1990), however administered during gestation, they have demonstrated teratogenic effects such as external, skeletal and vascular abnormalities (Cristofol et al, 1997;Navarro et al, 1998Navarro et al, , 1999Teruel et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Albendazole Sulphoxide on Embryonic, Foetal and Placental Parameters in Wistar Rats

2009

View full text Add to dashboard Cite

SUMMARY:The aim of this work was to evaluate the effect of albendazole sulphoxide (ABZSO), administered to Wistar rats during pregnancy on embryonic, foetal and placental parameters. A colpocytological control was performed daily and detection of spermatozoa in the vaginal smear was considered as day 0 of pregnancy. For the preimplantational study, ABZSO (10 mg/kg) was orally administered at day 2 of pregnancy; at day 4 the embryos were collected. For the postimplantational study, ABZSO (10 mg/kg) was orally administered by gavages at day 2, 6 or 10 of pregnancy (G2, G6 and G10 Groups respectively); the control group was administered the same volume of carboxymethylcellulose vehicle used to prepare the drug suspension. Fetuses were obtained from pregnant rats sacrificed on day 20 of gestation. Maternal body weight gains were analyzed using the one-way ANOVA test. Embryonic and foetal variables were analized on a per litter basis by the Kruskal-Wallis test. Skeletal anomalies were analyzed using an X 2 test. The significance level accepted was established at P<0.05. In the preimplantational analysis, the cleavage rate was lower in the experimental group (P<0.05). In the postimplantational analysis there were no differences in the net weight increase among females of the different groups (P>0.05). The number of fetuses and the foetal vesicles weight were lower in the G10 group (P<0.05). This group showed the highest percentage of resorptions (P<0.05) and fetuses morphologically abnormal. An increase in the number of bones affected in fetuses of G6 and G10 groups was observed. The most common malformations were at vertebral, costal and head level. Weights and placental diameters were lower in the G10 group (P<0.05). We conclude that ABZSO at the dose used in this study affects the cleavage rate in preimplantational embryo development, without interrupting pregnancy. Furthermore; the developmental toxicity is related to day of administration.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of Albendazole Sulphoxide on Embryonic, Foetal and Placental Parameters in Wistar Rats

2009

View full text Add to dashboard Cite

show abstract

“…The possible resorptions were analyzed after inspection of ovaries and uterus. All fetuses were analyzed for skeletal malformation according to Navarro et al (1998) [3]. The litter was considered as a unit of analysis.…”

Section: Methodsmentioning

confidence: 99%

Poster presentations

2009

Vet Pharm & Therapeutics

View full text Add to dashboard Cite

“…Among the anthelmintic agents, benzimidazole presents a broad spectrum of activity with high effectiveness and safety (Campbell, 1990) gestation, it has demonstrated teratogenic effects such as external, skeletal and vascular abnormalities (Cristófol et al, 1997;Navarro et al, 1998Navarro et al, , 1999Teruel et al, 2003Teruel et al, , 2009aCapece et al, 2003;Moreti et al, 2005). Several studies suggest that the capacity of benzimidazolic drugs to bind with the tubulin of cells is responsible for the toxic effects observed during gestation (Delatour & Parish, 1986;Piscopo & Smoak, 1997) and its efficacy is attributed to its active albendazole sulphoxide metabolite (ABZSO), owing to its higher systemic availability compared to ABZ (Delatour et al, 1984).…”

Section: Introductionmentioning

confidence: 99%

Albendazole Sulphoxide Administered Prior to Mating andits Relation with Fertilization and Mouse Embryo Development

2011

View full text Add to dashboard Cite

SUMMARY:The aim of this work was to evaluate the effect of albendazole sulphoxide (ABZSO) administered to Balb C mice prior to mating on fertilization rate and preimplantational embryo development. Twenty four female mice 5-8 weeks of age were superovulated by intraperitoneal injection of 7.5 UI of equine chorionic gonadotropin (eCG, Novormon®, Laboratorios Syntex S.A., Argentina); 48 h later they received 10 IU of human chorionic gonadotropin (hCG, Profasi®, Laboratorios Serono, Méjico) and were paired with males of proven fertility. Females received 100 mg/kg or 200 mg/kg of ABZSO orally at the time of hCG administration, prior to mating. The control group received carboxymethylcellulose, vehicle used to prepare the drug suspension. Pregnant females were killed by cervical dislocation at day 4 of pregnancy and non fertilized oocyte and embryos were flushed from uteri. The possible effects of ABZSO were evaluated considering the fertilization rate, the total number of collected embryos per female; the percentage of embryos morphologically normal; the differentiation rate (determined by the relation between the number of blastocyst and the total of morphologically normal embryos) and the cleavage rate determined by counting the nuclei. The variables were analyzed on a per litter basis using the Kruskal-Wallis test. The fertilization rate was lower in females administered ABZSO at a dose of 200 mg/kg (P<0.05). However, no statistically significant differences were found in the embryonic parameters after the administration of 100 mg/kg or 200 mg/kg of ABZSO compared to the untreated control group (P>0.05). In conclusion, a single acute exposure to ABZSO prior to mating at around the time of fertilization at a dose higher than the one usually administered in human and veterinary medicine affects the fertilization rate but it has no adverse effects on early embryo development.

show abstract

Anthelmintic induced congenital malformations in sheep embryos using netobimin

Cited by 31 publications

References 22 publications

Effects of Albendazole Sulphoxide on Embryonic, Foetal and Placental Parameters in Wistar Rats

Effects of Albendazole Sulphoxide on Embryonic, Foetal and Placental Parameters in Wistar Rats

Poster presentations

Albendazole Sulphoxide Administered Prior to Mating andits Relation with Fertilization and Mouse Embryo Development

Contact Info

Product

Resources

About