Anthelmintic-like activity of polyphenolic compounds and their interactions against the cattle nematode Cooperia punctata

Escareño-Díaz, S.; Alonso-Díaz, Miguel Ángel; Gives, Pedro Mendoza de; Castillo-Gallegos, Epigmenio; Fernex, Elke von Son-de

doi:10.1016/j.vetpar.2019.08.003

Cited by 17 publications

(19 citation statements)

References 35 publications

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“…Our procedure allowed the qualitative identification of quercetin as the main phytochemical present in the L . leucocephala extract, a phytochemical that has been previously reported to have anthelmintic activity against other parasites (Escareño‐Díaz et al 2019), and our findings are consistent with Tu et al (2021), who recently reported that quercetin had a 91% effectivity against Gyrodactylus spp.…”

Section: Discussionsupporting

confidence: 92%

Anthelmintic‐like Activity of Leucaena leucocephala Aqueous Extract Against Gyrodactylus spp. in Naturally Infected Tilapia Fingerlings

et al. 2021

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Aquaculture represents the fastest growing food‐producing sector worldwide. However, the intensification of fish farms has led to environmental, health, and welfare challenges. The monogenean ectoparasite Gyrodactylus spp. has been considered one of the most pathogenic ectoparasites affecting fish, and no specific control strategies besides formalin baths have been established. The use of bioactive plants has been recently proposed as an alternative method to control gyrodactylid infections. Leucaena leucocephala is a tropical legume that has been found with anthelmintic activity against both internal and external parasites of cattle. Therefore, the objective of this study was to assess the anthelmintic‐like activity of L. leucocephala aqueous extract against Gyrodactylus spp. in hybrid tilapia Oreochromis spp. fingerlings and its effect on host health through histopathological analysis. Five concentrations of L. leucocephala aqueous extract were employed (2.00, 1.00, 0.50, 0.25, and 0.125 mg/mL) to estimate the mean effective concentration (EC50). Distilled water and formalin (1:4,000) were used as negative and positive controls, respectively. The aqueous extract of L. leucocephala showed a dose‐dependent behavior, with an EC50 of 0.228 mg/mL. Quercetin was identified as the main phytochemical present in the extract. Postmortem analysis of fingerlings exposed to the plant extract had no treatment‐associated pathological findings, thus indicating that the use of L. leucocephala baths had no negative impact that could jeopardize the lives of treated animals. After the in vivo assessment, the aqueous extract of L. leucocephala appears to be a promising alternative to control gyrodactylid infection in tilapia.

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Section: Discussionsupporting

confidence: 92%

Anthelmintic‐like Activity of Leucaena leucocephala Aqueous Extract Against Gyrodactylus spp. in Naturally Infected Tilapia Fingerlings

et al. 2021

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“…lower efficacy). As it is unlikely that these flavonols would be improving egg hatching, an antagonistic relationship may be indicated here, which could be attributed to structural differences between the PAs and the other compounds [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of the anthelmintic efficacy of European heather extracts on Teladorsagia circumcincta and Trichostrongylus colubriformis egg hatching and larval motility

et al. 2022

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Background Gastrointestinal nematode (GIN) control is traditionally achieved with the use of anthelmintic drugs, however due to regulations in organic farming and the rise in anthelmintic resistance, alternatives are sought after. A promising alternative is the use of bioactive plant feeding due to the presence of plant secondary metabolites (PSMs) such as proanthocyanidins (PAs). This study focussed on the perennial shrub heather (Ericaceae family), a plant rich in PAs, highly abundant across Europe and with previously demonstrated anthelmintic potential. Methods In vitro assays were used to investigate heather’s anthelmintic efficacy against egg hatching and larval motility. Heather samples were collected from five European countries across two seasons, and extracts were tested against two GIN species: Teladorsagia circumcincta and Trichostrongylus colubriformis. Polyphenol group-specific ultraperformance liquid chromatography-tandem mass spectrometry analysis was performed to identify relevant polyphenol subgroups present, including the PA concentration and size and ratio of the subunits. Partial least squares analysis was performed to associate efficacy with variation in PSM composition. Results Heather extracts reduced egg hatching of both GIN species in a dose-dependent manner by up to 100%, while three extracts at the highest concentration (10 mg/ml) reduced larval motility to levels that were not significantly different from dead larvae controls. PAs, particularly the procyanidin type, and flavonol derivatives were associated with anthelmintic activity, and the particular subgroup of polyphenols associated with the efficacy was dependent on the GIN species and life stage. Conclusions Our results provide in vitro evidence that heather, a widely available plant often managed as a weed in grazing systems, has anthelmintic properties attributed to various groups of PSMs and could contribute to sustainable GIN control in ruminant production systems across Europe. Graphical Abstract

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“…In another study, phenolic compounds quercetin, rutin, coumarin, and caffeic acid were assayed against eggs of the cattle nematode Cooperia punctata [ 27 ]. A synergic effect was observed with the combination of caffeic acid with coumaric acid, quercetin, and rutin [ 29 ]. These studies, combined with the findings of the present work, support the hypothesis that the anthelmintic effect is attributed to the hydrocinnamic acid derivatives.…”

Section: Discussionmentioning

confidence: 99%

Phenolic Acids and Flavonoids from Pithecellobium dulce (Robx.) Benth Leaves Exhibit Ovicidal Activity against Haemonchus contortus

Olmedo-Juárez

Jimenez-Chino

Bugarin

et al. 2022

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Pithecellobium dulce (Robx.) Benth is an arboreal legume used in traditional medicine for the treatment of several ailments, including a number of intestinal disorders, and as a natural deworming. The objective of this study was to evaluate the ovicidal activity of a hydroalcoholic extract (HA-E) and its fractions (aqueous, Aq-F and organic, EtOAc-F) from P. dulce leaves, as well as subfractions (C1F1–C17) obtained from EtOAc-F against Haemonchus contortus eggs. The HA-E, Aq-F, and EtOAc-F were assessed at 0.03–5.00 mg/mL and subfractions (C1F1–C17) were assessed at 0.06–1.00 mg/mL. The HA-E and Aq-F showed an ovicidal activity close to 100% at 2.5 mg/mL, and EtOAc-F displayed the highest anthelmintic effect (100% at 0.25 mg/mL). Meanwhile, the sub-fractions with the highest ovicidal effect were C1F6, C1F9, C1F10, C1F11, and CIF13. The main compounds identified in the most active fractions (C1F9, C1F11, and C1F13) were kaempferol (1), quercetin (2), coumaric acid (3), ferulic acid (4), luteolin 7-O-rhamnoside (5), quercetin 3-O-rhamnoside (6), and a caffeoyl derivate (NI). The results indicate that P. dulce leaves exhibit a potent anthelmintic activity and contain bioactive compounds able to inhibit egg hatching in H. contortus. Therefore, this plant could be used for the control of gastrointestinal nematodes in small ruminants.

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Anthelmintic-like activity of polyphenolic compounds and their interactions against the cattle nematode Cooperia punctata

Cited by 17 publications

References 35 publications

Anthelmintic‐like Activity of Leucaena leucocephala Aqueous Extract Against Gyrodactylus spp. in Naturally Infected Tilapia Fingerlings

Anthelmintic‐like Activity of Leucaena leucocephala Aqueous Extract Against Gyrodactylus spp. in Naturally Infected Tilapia Fingerlings

Comparative analysis of the anthelmintic efficacy of European heather extracts on Teladorsagia circumcincta and Trichostrongylus colubriformis egg hatching and larval motility

Phenolic Acids and Flavonoids from Pithecellobium dulce (Robx.) Benth Leaves Exhibit Ovicidal Activity against Haemonchus contortus

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