Anthelmintic resistance and multidrug resistance in sheep gastro-intestinal nematodes in France, Greece and Italy

Geurden, Thomas; Hoste, Hervé; Jacquiet, Philippe; Traversa, Donato; Sotiraki, Smaragda; Regalbono, Antonio Frangipane di; Tzanidakis, Nikolaos; Kostopoulou, Despoina; Gaillac, Christie; Privat, Simon; Giangaspero, Annunziata; Zanardello, Claudia; Noé, Laura; Vanimisetti, Bindu; Bartram, David

doi:10.1016/j.vetpar.2014.01.016

Cited by 102 publications

(79 citation statements)

References 27 publications

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“…AHR in small ruminants has been reported in various regions of world: Argentina (EDDI et al, 1996); Uruguai (NARI et al, 1996;BONINO & MEDEROS, 2003), Paraguai (MACIEL et al, 1996); South Africa ( VAN WYK et al, 1997); Mexico (TORRES-ACOSTA et al, 2003); Australia (SUTER et al, 2004;PLAYFORD et al, 2014) (ZANZANI et al, 2014);Ireland (KEANE et al, 2014); France, Greece and Italy (GEURDEN et al, 2014). The countries with the largest number of cross-sectional studies over time are New Zealand, the United Kingdom and Brazil (FALZON et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Overview of anthelmintic resistance of gastrointestinal nematodes of small ruminants in Brazil

Salgado

Santos

2016

Rev. Bras. Parasitol. Vet.

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Frequent and inappropriate use of all classes of antiparasitic drugs in small ruminants has led to failures in their effectiveness, culminating in a global problem of anthelmintic resistance. Brazil stands out as one of the world's leaders in publications about anthelmintic resistance, and for having the most numerous reports of this resistance in small ruminants in the Americas. These studies have involved mainly the fecal egg count reduction test (FECRT) and its correlation with field management practices. In vivo effectiveness testing is conducted in areas where livestock is of greater economic significance, e.g., in the South (sheep) and Northeast (goats), or is important for research and economic centers, such as the Southeast (sheep). The most widely studied species is sheep, for which the widest range of drugs is also evaluated. Despite significant advances achieved in molecular research, laboratory analyses should include knowledge about the reality in the field so that they can become feasible for the producer. Moreover, molecular studies can be underpinned by the analysis of field studies, such as the maintenance of antiparasitic effectiveness over time and the mechanisms involved in this process.Keywords: Small ruminants, anthelmintic resistance, sheep, goat, gastrointestinal nematodes. ResumoO uso frequente e inapropriado de todas as classes de antiparasitários em pequenos ruminantes levou a falhas de eficácia, culminando na problemática global de resistência anti-helmíntica. O Brasil destaca-se como sendo um dos pioneiros nas publicações com resistência anti-helmíntica no mundo e por ter o maior número de relatos em pequenos ruminantes na América. Essas pesquisas envolvem principalmente o teste da redução da contagem de ovos nas fezes (TRCOF) e sua correlação com as práticas de manejo utilizadas no campo. Os estudos de testes de eficácia in vivo localizam-se em áreas onde há maior importância dos rebanhos como nas Regiões Sul (ovinos) e Nordeste (caprinos), ou com importância em polos de pesquisa e econômicos, como a região Sudeste (ovinos). Ovina é a espécie mais estudada e com maior gama de drogas avaliadas. Mesmo com grande avanço em pesquisas moleculares, as análises laboratoriais devem envolver o conhecimento da realidade do campo para que possam se tornar viáveis ao produtor. Além disso, a análise dos estudos de campo pode nortear estudos moleculares como, por exemplo, a manutenção da eficácia das drogas ao longo dos anos e os mecanismos envolvidos em tal processo.

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Section: Introductionmentioning

confidence: 99%

Overview of anthelmintic resistance of gastrointestinal nematodes of small ruminants in Brazil

Salgado

Santos

2016

Rev. Bras. Parasitol. Vet.

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“…BZ resistance is widely distributed in meat and dairy sheep industries in France as it has been demonstrated in the western (Hubert et al 1991;Chartier et al 1998), southwestern (Geurden et al 2014), and southeastern parts (Beugnet 1992) of the country. Recently, a macrocyclic lactone resistance has been demonstrated for the first time in a meat sheep farm from mainland France (Paraud et al 2016) where T. circumcincta was the IVM-and MOX-resistant species.…”

Section: Discussionmentioning

confidence: 99%

“…In almost all sheep markets in Europe, AR to benzimidazoles (BZ) has been described (Papadopoulos et al 2012); however, ivermectin (IVM) and moxidectin (MOX) resistances are still less frequent in Europe than in the southern hemisphere with the noticeable exception of the UK (Bartley et al 2006) and Ireland (Keane et al 2014). BZ resistance has been frequently described in mainland France (Chartier et al 1998;Geurden et al 2014). The first case of cross-resistance to moxidectin and ivermectin has been detected recently on a meat sheep farm in France (Paraud et al 2016), but limited studies have addressed the issue of IVM resistance in this country.…”

Section: Introductionmentioning

confidence: 99%

A real-time PCR approach to identify anthelmintic-resistant nematodes in sheep farms

et al. 2017

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Resistance to fenbendazole, ivermectin, and moxidectin was explored by a fecal egg count reduction test in four meat sheep flocks in southwestern France where anthelmintic resistance was suspected. The FECR test results of the present study confirmed the presence of benzimidazole resistance in three out of the four farms and the presence of ivermectin resistance in one flock. In addition, a suspicion of moxidectin resistance was shown in this latter farm. Both conventional morphological and molecular identifications were performed on larval cultures before and after the treatment in the studied farms. A high positive correlation was found between the number of larvae counted under binocular microscope and the number of larvae estimated by the qPCR analysis (R 2 = 0.88) and a high Cohen's Kappa value (0.91) in the detection of strongylid larvae in larval cultures. According to qPCR results, Trichostrongylus species demonstrated high levels of BZ resistance and Teladorsagia circumcincta was involved in the IVM resistance in one farm. The molecular procedures used in this study have the potential to be beneficial for anthelmintic resistance surveillance in sheep industry.

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“…However, anthelmintic resistance of GINs is an expanding problem and is of major concern in many countries (Kaplan 2004;Sutherland and Leathwick 2011;Kaplan and Vidyashankar 2012;Torres-Acosta et al, 2012a;Geurden et al, 2014). Additionally, anthelmintic resistance is an increasing problem not only in small ruminants (Kaplan 2004;Kaplan and Vidyashankar 2012) but in cattle (Sutherland and Leathwick 2011;Cotter et al, 2015) and horses (Matthews 2014;Nielsen et al, 2014).…”

Section: Status Of Anthelmintic Resistance and Management Strategiesmentioning

confidence: 99%

“…Since the first case of resistance was observed in the early 1960s, resistance of GINs to the three groups of anthelmintics (benzimidazoles, nicotinic agonists, and macrolytic lactones) has become prevalent all over the world (Kaplan 2004;Wolstenholme et al, 2004;Fleming et al, 2006;Kaplan and Vidyashankar 2012;Cotter et al, 2015). Additionally, multiple resistance in single nematode strains also is of concern (Taylor et al, 2009;Papadopoulos et al, 2012;Torres-Acosta et al, 2012a;Geurden et al, 2014). However, the levels of resistance of GINs against anthelmintics may differ between areas (Kaplan and Vidyashankar 2012;Papadopoulos et al, 2012;Torres-Acosta et al, 2012a).…”

Section: Status Of Anthelmintic Resistance and Management Strategiesmentioning

confidence: 99%

Effects of dietary protein supplementation and plane of nutrition on the resistance and the resilience of Boer goats against artificial Haemonchus contortus infection under confined conditions

Can¹

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The effects of protein and/ or energy supplementation have been well studied in sheep. However, the few pen studies conducted with goats have failed to identify the role of protein supplementation and combinations with energy supplements against H. contortus infection.The general objective of this thesis was to investigate the effects of protein supplementation and plane of nutrition on resistance and resilience of Boer goats to infection from Haemonchus contortus. To achieve this the first experiment was designed to determine the appropriate amount of additional dietary protein (by having three groups of mature goats effectively fed three different levels of dietary protein) required by nematode free animals given a single relatively high dose of infective H. contortus larvae, to not become infected or at least develop resistance and/or resilience. It was assumed, based on the scientific literature, that a single relatively high dose of infective larvae would elicit strong pathogenic effects where additional dietary protein would give some assistance to the does with their defense to Haemonchus. As often is the case with research on goats the results were not unequivocally clear.Based on these results it was clear that a single dose of infective larvae was insufficient to become established and that these animals had already developed some level of immune response and thus it was assumed, again based on the scientific literature, to elicit strong pathogenic effects the animals tested needed to be younger (more naïve to Haemonchus) and with greater i.e. continued exposure to infective larvae as would happen under grazing conditions (trickle infection) such that different quantities of dietary protein would give these animals different responses to infection by Haemonchus. Again the responses by the goats only indicated subtle differences in immune responses against trickle H. contortus L3 infection. These results, again unexpected, indicated that level of intake (or more specifically plane of nutrition, prior to or during exposure) may be at least as important as protein supplementation in reasonably well fed animals; and or that the method of infection (oral drenching with L3 larvae) was possibly an unreliable method of infection requiring a more consistent method of infection. As such the third experiment involved nematode free mature animals (the only animals available at that time) fed on very different planes of nutrition preceding and during the experiment given relatively high trickle doses of H. contortus larvae via intra-ruminal infection.Overlaid across these experiments were constraints imposed on the amount of infective L3 larvae used to achieve infection; 100 L3 larvae per kg live-weight as a single dose was the maximum initially approved by the University Animal Ethics committee, as they had concerns about animals dying, they also stipulated the use of oral drenching for infective larvae. It was only after these methods failed ii to elicit a strong response was approval given by the Univers...

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Anthelmintic resistance and multidrug resistance in sheep gastro-intestinal nematodes in France, Greece and Italy

Cited by 102 publications

References 27 publications

Overview of anthelmintic resistance of gastrointestinal nematodes of small ruminants in Brazil

Overview of anthelmintic resistance of gastrointestinal nematodes of small ruminants in Brazil

A real-time PCR approach to identify anthelmintic-resistant nematodes in sheep farms

Effects of dietary protein supplementation and plane of nutrition on the resistance and the resilience of Boer goats against artificial Haemonchus contortus infection under confined conditions

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