Anther- and Pollen-Expressed Genes

Mascarenhas, Joseph P.

doi:10.1007/978-3-7091-6950-6_6

Cited by 27 publications

(12 citation statements)

References 84 publications

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“…Biochemical analysis showed, moreover, that the mRNAs, ribosomes, and tRNAs required for germination are synthesized during pollen maturation and persist in the pollen grain until they are utilized for translation during the germination process (reviewed in Mascarenhas, 1988). In most species, the block in tube growth induced by actinomycin D occurs later than that induced with protein synthesis inhibitors (reviewed in Mascarenhas, 1975).…”

Section: Regulation Of Gene Actlvlty In the Germinating Pollen Graln mentioning

confidence: 99%

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Molecular Mechanisms of Pollen Tube Growth and Differentiation

Mascarenhas¹

1993

The Plant Cell

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Section: Regulation Of Gene Actlvlty In the Germinating Pollen Graln mentioning

confidence: 99%

“…The evidence seems to indicate that the RNA that is synthesized is not rRNA or tRNA (reviewed in Mascarenhas, 1988). Rather, the RNAs that are synthesized during Tradescantia pollen germination and tube growth appear to be mRNAs or mRNA precursors (Mascarenhas et al, 1974).…”

Section: Regulation Of Gene Actlvlty In the Germinating Pollen Graln mentioning

confidence: 99%

Molecular Mechanisms of Pollen Tube Growth and Differentiation

Mascarenhas¹

1993

The Plant Cell

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150

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“…cDNA clones corresponding co, cortex; pi, pith; ph, phloem; tre, tracheary elements; x, xylem; xp, xylem parenchyma. to mRNAs expressed in all sporophytic cell layers of the anthers, except the tapetum, have been isolated from tobacco (Goldberg, 1988;Drews and Goldberg, 1989), and tapetalspecific cDNA clones have been characterized in tomato (Smith etal., 1987) and tobacco (Goldberg, 1988). Also, pollen-specific cDNA clones have been isolated from corn and spiderwort (Mascarenhas, 1988;Hanson et al, 1989). McCormick et al (1987) have described the cloning and characterization of anther-specific cDNA clones from tomato.…”

Section: Osm::gus Gene Is Highly Expressed During Natural Desiccationmentioning

confidence: 99%

Regulation of the Osmotin Gene Promoter

Kononowicz¹,

Nelson²,

Singh³

et al. 1992

The Plant Cell

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By introducing a chimeric gene fusion of the osmotin promoter and [beta]-glucuronidase into tobacco by Agrobacterium-mediated transformation, we have demonstrated a very specific pattern of temporal and spatial regulation of the osmotin promoter during normal plant development and after adaptation to NaCl. We have found that the osmotin promoter has a very high natural level of activity in mature pollen grains during anther dehiscence and in pericarp tissue at the final, desiccating stages of fruit development. GUS activity was rapidly lost after pollen germination. The osmotin promoter thus appears to be unique among active pollen promoters described to date in that it is active only in dehydrated pollen. The osmotin promoter was also active in corolla tissue at the onset of senescence. Adaptation of plants to NaCl highly stimulated osmotin promoter activity in epidermal and cortex parenchyma cells in the root elongation zone; in epidermis and xylem parenchyma cells in stem internodes; and in epidermis, mesophyll, and xylem parenchyma cells in developed leaves. The spatial and temporal expression pattern of the osmotin gene appears consistent with both osmotic and pathogen defense functions of the gene.

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“…In most of the plants studied, the mRNAs or proteins required for germination are already present in the mature pollen grain, and new protein synthesis is required only for growth of the pollen tubes following germination. Although the pollen of most plants studied contains ribosomes, mRNAs and tRNAs, new RNA synthesis does occur during pollen germination and tube growth (Mascarenhas, 1988;Ursin et al, 1989). The nature of pollen tube growth is different from other plant cell types.…”

Section: Introductionmentioning

confidence: 99%

Specific distribution of mRNAs in maize growing pollen tubes observed by whole‐mount in situ hybridization with non‐radioactive probes

et al. 1995

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The distribution of a number of specific mRNAs has been observed in maize growing pollen tubes. Whole-mount in situ hybridization using digoxygenin-labelled RNA probes has been tested. The technique appears to be a simple, rapid and reliable method in this system, in immature anthers and also in other tissues. Results for three probes are presented. They correspond to a hydroxyproline-rich glycoprotein (HRGP), an abundant component of the maize cell wall, to an a-tubulin (encoded by the Tuba 1 gene) highly expressed in the radicular system of the plant and also in pollen, and to an isoform of the malic enzyme, involved in the basic metabolism of the plant. The mRNAs corresponding to these three proteins are differently distributed in the germinating pollen. While HRGP mRNA is only present in the tube, malic enzyme mRNA is only present in the body of the pollen cell, and a-tubulin mRNA is present in both parts of the cell but shows a higher accumulation in the tip of the pollen tube.

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Anther- and Pollen-Expressed Genes

Cited by 27 publications

References 84 publications

Molecular Mechanisms of Pollen Tube Growth and Differentiation

Molecular Mechanisms of Pollen Tube Growth and Differentiation

Regulation of the Osmotin Gene Promoter

Specific distribution of mRNAs in maize growing pollen tubes observed by whole‐mount in situ hybridization with non‐radioactive probes

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