Anther culture as an effective tool in winter wheat (Triticum aestivum L.) breeding

Lantos, Csaba; Pauk, János

doi:10.1134/s102279541608007x

Cited by 28 publications

(47 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, technically, a simpler method is cultivation of anthers collected from the transgenic plants rather than microspore cultures. Many studies reported that androgenesis has a high potential for biological and molecular studies, as well as for plant breeding of crop plants [ 13 , 14 , 15 , 16 ]. Conventional selection protocols for obtaining pure homozygous transgenic lines is approximately 10–11 years in the monocot crop species, barley and wheat.…”

Section: Discussionmentioning

confidence: 99%

Homozygous Transgenic Barley (Hordeum vulgare L.) Plants by Anther Culture

Ohnoutková

Vlčko

2020

Plants

View full text Add to dashboard Cite

Production of homozygous lines derived from transgenic plants is one of the important steps for phenotyping and genotyping transgenic progeny. The selection of homozygous plants is a tedious process that can be significantly shortened by androgenesis, cultivation of anthers, or isolated microspores. Doubled haploid (DH) production achieves complete homozygosity in one generation. We obtained transgenic homozygous DH lines from six different transgenic events by using anther culture. Anthers were isolated from T0 transgenic primary regenerants and cultivated in vitro. The ploidy level was determined in green regenerants. At least half of the 2n green plants were transgenic, and their progeny were shown to carry the transgene. The process of dihaploidization did not affect the expression of the transgene. Embryo cultures were used to reduce the time to seed of the next generation. The application of these methods enables rapid evaluation of transgenic lines for gene function studies and trait evaluation.

show abstract

Section: Discussionmentioning

confidence: 99%

Homozygous Transgenic Barley (Hordeum vulgare L.) Plants by Anther Culture

Ohnoutková

Vlčko

2020

Plants

View full text Add to dashboard Cite

show abstract

“…From the prepared spikes, 300 anthers were isolated in each 90 mm diameter glass Petri dishes containing 12 ml ‘W14mf’ induction medium [39,40]. On the first three days of culture, the Petri dish were incubated at 32 °C to apply heat shock treatment.…”

Section: Methodsmentioning

confidence: 99%

Utilization of in Vitro Anther Culture in Spelt Wheat Breeding

Lantos

Purgel

Ács

et al. 2019

Plants

View full text Add to dashboard Cite

The efficiency of in vitro anther culture was screened in a full diallel population of four spelt wheat genotypes and ten F1 hybrids. Genotype dependency was observed based on the data of embryo-like structures (ELS), green-, albino plantlets. In the diallel population and ten F1 hybrids, the green plantlets production ranged from 13.75 to 85.00 and from 6.30 to 51.00, respectively. The anther culture-derived plants of F1 hybrids were grown up in the nursery. At the harvest, 436 spontaneous doubled haploid (DH) plants were identified among the 1535 anther culture-derived transplanted and grown up individual plants. The mean of spontaneous rediploidization was 28.4% which ranged from 9.76% to 54.24%. In two consecutive years, the agronomic values of ‘Tonkoly.pop1’ advanced line were compared with seven DH lines of ‘Tonkoly.pop1’ in the nursery. The DH lines achieved competitive values in comparison with ‘Tonkoly.pop1’ advanced line based on the 11 measured parameters (heading date, plant height, yield, hardness, width and length of seed, TKW, hulling yield, flour yield, protein and wet gluten content). These observations presage the efficient utilization of anther culture in spelt wheat breeding.

show abstract

“…Les résultats de l'analyse de la variance n'ont pas mis en évidence une interaction génotype x milieu de culture. Un résultat similaire a été obtenu par Lantos et Pauk (2016). Toutefois, l'androgenèse des céréales est souvent caractérisée par une interaction génotype x milieu d'induction (Fadel et Wenzel, 1990 ;Foroughi-Wehr, 1993 ;Jauhar, 2003).…”

Section: Influence Du Génotype Et Du Milieu De Culture Sur L'inductiounclassified

Response of some Tunisian bread wheat varieties (Triticum aestivum L.) to anther culture system

Ltifi¹,

Sahli²,

Brahmi³

et al. 2018

LSJ

View full text Add to dashboard Cite

This study focusedon wheat haploid production by anther culture. The main purpose wasto compare the in vitro androgenesis response often wheat Tunisian bread wheat varieties on two different induction media. The anthers were introduced in vitro when microspores were in the middle uninucleate stage and obtainedfrom treated spikes at 4° C for two weeks. The culture media 190-2 and BAC3 were used. Plants regenerated from induced embryos and calli were grown on the 190-2 medium without any growth regulators. The response to anther culture was different between the studied varieties. Androgenesis induction was better on the 190-2 medium than on BAC3 medium. In addition, the development of calli and the regeneration of embryos were better on 190-2 medium than on BAC3. Both green plantlets and albina were regenerated from induced embryos on the 190-2 medium, while no green plant regenerated from the embryos growingon BAC3 medium. Four varieties over ten studied gave good number of green plants. These results indicatedthat 190-2 medium was most favorable for the in vitro androgenesis of the Tunisian bread wheat varieties.

show abstract

Anther culture as an effective tool in winter wheat (Triticum aestivum L.) breeding

Cited by 28 publications

References 30 publications

Homozygous Transgenic Barley (Hordeum vulgare L.) Plants by Anther Culture

Homozygous Transgenic Barley (Hordeum vulgare L.) Plants by Anther Culture

Utilization of in Vitro Anther Culture in Spelt Wheat Breeding

Response of some Tunisian bread wheat varieties (Triticum aestivum L.) to anther culture system

Contact Info

Product

Resources

About