Anthocyanin accumulation and expression pattern of anthocyanin biosynthesis genes in developing wheat coleoptiles

Ahmed, Nasar Uddin; Maekawa, Masahiko; Noda, Kazuhiko

doi:10.1007/s10535-009-0043-x

Cited by 33 publications

(20 citation statements)

References 20 publications

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“…These genes were expressed in a highly correlative way with MrMYB1 expression, as cultivar, tissue and light regulation were consistent with a primary effect on MrMYB1, which then affects the pathways expression. CHS and CHI were least influenced by tissue, cultivar or fruit bagging treatments, suggesting its non-rate limiting role in anthocyanin biosynthesis, as has been observed in some other plant species (Ju et al 1995;Boss et al 1996;Lo Piero et al 2005;Ahmed et al 2009;Yuan et al 2009). The EBG have been separated by such behavior from LBG in species such as grape, wheat and gentian (Boss et al 1996;Kobayashi et al 2002;Nakatsuka et al 2008;Ahmed et al 2009).…”

Section: The Bayberry Anthocyanin Pathwaymentioning

confidence: 53%

Coordinated regulation of anthocyanin biosynthesis in Chinese bayberry (Myrica rubra) fruit by a R2R3 MYB transcription factor

Niu

Zhang

et al. 2010

Planta

253

166

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Chinese bayberry (Myrica rubra) is a fruit crop with cultivars producing fruit ranging from white (Shuijing, SJ) to red (Dongkui, DK) and dark red-purple (Biqi, BQ), as a result of different levels of anthocyanin accumulation. Genes encoding the anthocyanin biosynthesis enzymes chalcone synthase, chalcone isomerase, flavanone 3-hydroxylase (F3H), flavonoid 3'-hydroxylase (F3'H), dihydroflavonol 4-reductase (DFR), anthocyanidin synthase (ANS) and UDPglucose: flavonoid 3-O-glucosyltransferase (UFGT), as well as MrMYB1, a R2R3 MYB transcription factor homologous to known activators of anthocyanin biosynthesis, were isolated from ripe fruit of BQ. Differences in mRNA abundance of MrF3H, MrF3'H, MrDFR1, MrANS and MrUFGT were highly correlated with differential accumulation of anthocyanins between cultivars, suggesting coordinated regulation by transcription factors. The transcript level of MrMYB1 was strongly associated with the anthocyanin content in ripe fruit of the three cultivars, as well as different anthocyanin containing tissues of BQ fruit. Fruit bagging strongly inhibited anthocyanin accumulation in fruit as well as the expression of all anthocyanin biosynthetic genes and MrMYB1. Overexpression of MrMYB1 stimulated both anthocyanin accumulation and activated an Arabidopsis-DFR promoter in tobacco (Nicotiana tabacum). MrMYB1d, an allele with a 1 bp deletion at nucleotide 30 of coding sequence, was observed in SJ and DK fruit, suggesting that a nonsense mutation of the MYB1 protein may be responsible for no or low expression of MYB1 in the white and red fruit. These results show that coordinated expression of multiple biosynthetic genes is involved in anthocyanin accumulation in Chinese bayberry fruit, and this is regulated by MrMYB1.

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Section: The Bayberry Anthocyanin Pathwaymentioning

confidence: 53%

Coordinated regulation of anthocyanin biosynthesis in Chinese bayberry (Myrica rubra) fruit by a R2R3 MYB transcription factor

Niu

Zhang

et al. 2010

Planta

253

166

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“…Auxin transport and accumulation may also have an influence in somatic embryo differentiation in Brachiaria. Ahmed et al (2009) showed anthocyanin accumulation in epidermal cells of wheat coleoptiles germinated under white light, suggesting that light is one of the most important environmental stimuli regulating anthocyanin accumulation. Moreover, they demonstrated that anthocyanin biosynthesis is tissue specific and highly conserved among plant species.…”

Section: Resultsmentioning

confidence: 97%

Somatic embryogenesis and organogenesis in apomictic and sexual Brachiaria brizantha

Cabral

Carneiro

Lacerda

et al. 2011

Plant Cell Tiss Organ Cult

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Brachiaria brizantha (syn. Urochloa brizantha) is an important tropical forage grass widely cultivated in Brazil. In order to optimize tissue culture conditions for B. brizantha, in vitro culture of mature seeds, basal segments and leaf segments from in vitro plants of an apomictic and a sexual genotype of B. brizantha was performed. When cultured on different media, leaf segments yielded nonembryogenic calluses which formed several roots. Friable calluses from mature seeds and basal segments explants incubated on Murashige and Skoog medium supplemented with 2,4-dichlorophenoxyacetic acid and 6-benzyladenine yielded 80% compact and nodular embryogenic structures. Calluses with such compact embryogenic structures were highly regenerable upon transfer to medium supplemented with kinetin and naphthalene acetic acid. They produced isolated somatic embryos, multiple fused scutelli or isolated scutellum with polyembryos that germinated into isolated or multiple shoots. Green and morphologically normal plants were obtained for the two genotypes. Changing the media from pH 5.8 to pH 4.0 increased the number of explants that formed calluses as well as the number of shoots per explant. When embryogenic calluses from mature seeds were successively sub-cultured for 4 months, aiming at repetitive somatic embryogenesis, all the regenerated plants were albinos. The embryogenic nature of the compact structure was confirmed by scanning electron microscopy.

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“…The phenylpropanoid pathway of anthocyanin synthesis has been well characterized, and there are several reviews that describe it in detail (Jaakola et al 2002;Winkel-Shirley 2006;Vogt 2010;Teixeira et al 2013). Anthocyanin synthesis occurs mainly in epidermal cells of different organs such as stem, leaves, flowers, and fruits (Jackson et al 1992;Huits et al 1994;Bae and Kim 2006;Ahmed et al 2009;Gould et al 2009). Although some authors (Pelletier and Shirley 1996;Buer and Muday 2004;Buer et al 2007) have indicated that roots and tissues grown in the dark are largely incapable of synthesizing significant levels of anthocyanins because the biosynthetic enzymes are all light dependent, other authors (Buer et al 2007;Neufeld et al 2011) have demonstrated that in Galax urceolata and Ipomoea batatas root tissues anthocyanin biosynthesis can occur without light.…”

Section: Overview Of Biosynthesis and Transport Of Anthocyaninsmentioning

confidence: 99%

Evaluating the involvement and interaction of abscisic acid and miRNA156 in the induction of anthocyanin biosynthesis in drought-stressed plants

González‐Villagra

Kurepin

2017

Planta

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Main conclusion ABA is involved in anthocyanin synthesis through the regulation of microRNA156, augmenting the level of expression of anthocyanin synthesisrelated genes and, therefore, increasing anthocyanin level.Drought stress is the main cause of agricultural crop loss in the world. However, plants have developed mechanisms that allow them to tolerate drought stress conditions. At cellular level, drought stress induces changes in metabolite accumulation, including increases in anthocyanin levels due to upregulation of the anthocyanin biosynthetic pathway. Recent studies suggest that the higher anthocyanin content observed under drought stress conditions could be a consequence of a rise in the abscisic acid (ABA) concentration. This plant hormone crosses the plasma membrane by specific transporters, and it is recognized at the cytosolic level by receptors known as pyrabactin resistance (PYR)/regulatory component of ABA receptors (PYR/RCARs) that regulate downstream components. In this review, we discuss the hypothesis regarding the involvement of ABA in the regulation of microRNA156 (miRNA156), which is upregulated as part of dehydration stress responsiveness in different species. The miRNA156 upregulation produces a greater level of anthocyanin gene expression, forming the multienzyme complex that will synthesize an increased level of anthocyanins at the cytosolic face of the rough endoplasmic reticulum (RER). After synthesis, anthocyanins are transported from the RER to the vacuole by two possible models of transport: (1) membrane vesicle-mediated transport, or (2) membrane transporter-mediated transport. Thus, the aim was to analyze the recent findings on synthesis, transport and the possible mechanism by which ABA could increase anthocyanin synthesis under drought stress conditions potentially throughout microRNA156 (miRNA156).

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Anthocyanin accumulation and expression pattern of anthocyanin biosynthesis genes in developing wheat coleoptiles

Cited by 33 publications

References 20 publications

Coordinated regulation of anthocyanin biosynthesis in Chinese bayberry (Myrica rubra) fruit by a R2R3 MYB transcription factor

Coordinated regulation of anthocyanin biosynthesis in Chinese bayberry (Myrica rubra) fruit by a R2R3 MYB transcription factor

Somatic embryogenesis and organogenesis in apomictic and sexual Brachiaria brizantha

Evaluating the involvement and interaction of abscisic acid and miRNA156 in the induction of anthocyanin biosynthesis in drought-stressed plants

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