Anthocyanin fingerprinting and dynamics in differentially pigmented exotic soybean genotypes using modified HPLC–DAD method

Krishnan, Veda; Rani, Raja; Pushkar, Suchithra; Lal, S. K.; Srivastava, Sudhir; Kumari, Sweta; Vinutha, T.; Dahuja, Anil; Praveen, Shelly; Sachdev, Archana

doi:10.1007/s11694-020-00443-y

Cited by 13 publications

(11 citation statements)

References 30 publications

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“…Based on this fingerprinting, it is possible to recognize the adulteration and/or sophistication of particular products that claim to contain a certain fruit. This method allows for the detection of a misidentification of the initial plant raw material or adulteration with other plants [3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Genetic Differentiation in Anthocyanin Content among Berry Fruits

Ponder

Hallmann

Kwolek

et al. 2021

CIMB

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Anthocyanins are widely distributed secondary metabolites that play an essential role in skin pigmentation of many plant organs and microorganisms. Anthocyanins have been associated with a wide range of biological and pharmacological properties. They are also effective agents in the prevention and treatment of many chronic diseases. Berries are particularly abundant in these compounds; therefore, their dietary intake has health-promoting effects. The aim of this study was to identify and determine the anthocyanin content in selected species and cultivars of berry fruits, such as raspberry, blackberry, red currant, blackcurrant, and highbush blueberry, widely consumed by Europeans. The concentrations of anthocyanins were determined by HPLC, identifying individual compounds: cyanidin-3-O-glucoside, pelargonidin-3-O-glucoside, delphinidin-3-O-glucoside, delphinidin-3-O-rutinoside, cyanidin-3-O-rutinoside, delphinidin-3-O-galactoside, cyanidin-3-O-galactoside, and malvidin-3-O-galactoside. The experimental data showed that the selected species and cultivars of berry fruits differ significantly in the contents of anthocyanins. Among all species tested, blackberry and blackcurrant were characterized significantly by the highest content of anthocyanins (sum), while the lowest content was found in red currant fruits. Additionally, the content of individual anthocyanin compounds in particular species and cultivars was also different. Considering the high content of anthocyanins and their potential positive impact on human health and protection against disease, berries should be part of healthy nutrition.

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Section: Introductionmentioning

confidence: 99%

Genetic Differentiation in Anthocyanin Content among Berry Fruits

Ponder

Hallmann

Kwolek

et al. 2021

CIMB

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“…Therefore, the leaves of small-seeded cultivars such as KA and AR may serve as a food source. Black soybean seeds contain high levels of anthocyanins . Anthocyanins share a biosynthetic pathway with kaempferol and quercetin.…”

Section: Resultsmentioning

confidence: 99%

“…Black soybean seeds contain high levels of anthocyanins. 42 Anthocyanins share a biosynthetic pathway with kaempferol and quercetin. Cyanidin, the most abundant anthocyanin in black soybeans, is synthesized from dihyroquercetin, a precursor of quercetin.…”

Section: Resultsmentioning

confidence: 99%

Metabolite Changes in Soybean (Glycine max) Leaves during the Entire Growth Period

Park,

Lee,

Park

et al. 2023

ACS Omega

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Although soybean (Glycine max) leaves generate building blocks to produce seeds, a comprehensive understanding of the metabolic changes in soybean leaves during the entire growth stages is limited. Here, we investigated the metabolite changes in soybean leaves from five cultivars among four vegetative (V) and eight reproductive (R) stages using metabolite profiling coupled with chemometrics. Principal component analysis (PCA) of all samples showed a clear separation by growth stage. The total amount of monosaccharides and organic acids for energy production were highly detected in the V stage samples, accumulating in concentrations 2.5 and 1.7 times higher than in the R stage samples, respectively. The results of partial least-squares-discriminant analysis (PLS-DA) revealed a clear separation from R1 to R5 by the first PLS, suggesting significant alterations in the metabolic networks up to R5. After flowering, the stage of seed formation, R5, was associated with lower levels of most amino acids and an accumulation of phytosterols. The negative correlation observed between amino acids and phytosterol levels suggests a sophisticated coordination between carbon and nitrogen metabolism in plant, ensuring and supporting optimal growth (r = −0.50085, P = 0.0001). In addition, R-stage samples had decreased monosaccharide levels, indicating redistribution to seeds and senescence-related metabolite changes. Thus, metabolite profiling coupled with chemometrics could be a useful tool for investigating alterations in metabolic networks during various plant growth and development stages. Furthermore, we observed variations in flavonoid contents among the different cultivars. The results could be a basis of further studies on the source−sink interactions in the plant system.

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“…Analysis of anthocyanin compounds (C3G and P3G) in the BR extract was performed using HPLC technique (Infinity 1260, Agilent Technologies, Santa Clara, CA, USA) using reversed-phase C18 column (Zorbax Eclipse Plus C18, 250 mm × 4.6 mm, 5 µm, supplied from Agilent Technologies, Santa Clara, CA, USA). The HPLC protocol was followed from a previously described method [30]. The mobile phase was composed of mobile phase A (0.4% trifluoroacetic acid in distilled water) and mobile phase B (0.45% trifluoroacetic acid in acetonitrile) under isocratic conditions.…”

Section: Identification Of Active Anthocyanin Compounds In Br Extract...mentioning

confidence: 99%

Cyanidin-3-O-glucoside and Peonidin-3-O-glucoside-Rich Fraction of Black Rice Germ and Bran Suppresses Inflammatory Responses from SARS-CoV-2 Spike Glycoprotein S1-Induction In Vitro in A549 Lung Cells and THP-1 Macrophages via Inhibition of the NLRP3 Inflammasome Pathway

et al. 2022

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Black rice is a functional food that is high in anthocyanin content, primarily C3G and P3G. It possesses nutraceutical properties that exhibit a range of beneficial effects on human health. Currently, the spike glycoprotein S1 subunit of SARS-CoV-2 (SP) has been reported for its contribution to pathological inflammatory responses in targeting lung tissue and innate immune cells during COVID-19 infection and in the long-COVID phenomenon. Our objectives focused on the health benefits of the C3G and P3G-rich fraction of black rice germ and bran (BR extract) on the inhibition of inflammatory responses induced by SP, as well as the inhibition of NF-kB activation and the NLRP3 inflammasome pathway in an in vitro model. In this study, BR extract was identified for its active anthocyanins, C3G and P3G, using the HPLC technique. A549-lung cells and differentiated THP-1 macrophages were treated with BR extract, C3G, or P3G prior to exposure to 100 ng/mL of SP. Their anti-inflammatory properties were then determined. BR extract at concentrations of 12.5–100 μg/mL exhibited anti-inflammation activity for both A549 and THP-1 cells through the significant suppression of NLRP3, IL-1β, and IL-18 inflammatory gene expressions and IL-6, IL-1β, and IL-18 cytokine secretions in a dose-dependent manner (p < 0.05). It was determined that both cell lines, C3G and P3G (at 1.25–10 μg/mL), were compatibly responsible for the significant inhibition of SP-induced inflammatory responses for both gene and protein levels (p < 0.05). With regard to the anti-inflammation mechanism, BR extract, C3G, and P3G could attenuate SP-induced inflammation via counteraction with NF-kB activation and downregulation of the inflammasome-dependent inflammatory pathway proteins (NLRP3, ASC, and capase-1). Overall, the protective effects of anthocyanins obtained from black rice germ and bran can be employed in potentially preventive strategies that use pigmented rice against the long-term sequelae of COVID-19 infection.

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Anthocyanin fingerprinting and dynamics in differentially pigmented exotic soybean genotypes using modified HPLC–DAD method

Cited by 13 publications

References 30 publications

Genetic Differentiation in Anthocyanin Content among Berry Fruits

Genetic Differentiation in Anthocyanin Content among Berry Fruits

Metabolite Changes in Soybean (Glycine max) Leaves during the Entire Growth Period

Cyanidin-3-O-glucoside and Peonidin-3-O-glucoside-Rich Fraction of Black Rice Germ and Bran Suppresses Inflammatory Responses from SARS-CoV-2 Spike Glycoprotein S1-Induction In Vitro in A549 Lung Cells and THP-1 Macrophages via Inhibition of the NLRP3 Inflammasome Pathway

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