Anthocyanin galactosyltransferase from Aralia cordata, cDNA cloning and characterization

Nagashima, Shigeyuki; Okamoto, Aya; Suzuki, Hideyuki; Asada, Yujiro; Kondo, Tohru; Yoshikawa, Takafumi

doi:10.5511/plantbiotechnology.21.191

Cited by 10 publications

(8 citation statements)

References 15 publications

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“…B), a 44‐amino‐acid sequence found in most plant UFGT enzymes (Yonekura‐Sakakibara and Hanada ). AcUFGT3a shared 42–99% CDS identify with other plant UFGTs (Nagashima et al ). In particular, it showed 99% CDS identity to AcF3GT1 of the cultivar ‘HD22’ (Montefiori et al ).…”

Section: Resultsmentioning

confidence: 99%

Biochemical and functional characterization of AcUFGT3a, a galactosyltransferase involved in anthocyanin biosynthesis in the red‐fleshed kiwifruit (Actinidia chinensis)

Liu

Zhou

et al. 2017

Physiologia Plantarum

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Much of the diversity of anthocyanin pigmentation in plant tissues is due to the action of glycosyltransferases, which attach sugar moieties to the anthocyanin aglycone. This step can increase both their solubility and stability. We investigated the pigmentation of the outer and inner pericarps of developing fruits of the red-fleshed kiwifruit Actinidia chinensis cv. 'Hongyang'. The results show that the red color of the inner pericarp is due to anthocyanin. Based on expression analyses of structural genes, AcUFGT was shown to be the key gene involved in the anthocyanin biosynthetic pathway. Expression of AcUFGT in developing fruit paralleled changes in anthocyanin concentration. Thirteen putative UFGT genes, including different transcripts, were identified in the genome of 'Hongyang'. Among these, only the expression of AcUFGT3a was found to be highly consistent with anthocyanin accumulation. Fruit infiltrated with virus-induced gene silencing showed delayed red colorations, lower anthocyanin contents and lower expressions of AcUFGT3a. At the same time, transient overexpression of AcUFGT3a in both Actinidia arguta and green apple fruit resulted in higher anthocyanin contents and deeper red coloration. In vitro biochemical assays revealed that recombinant AcUFGT3a recognized only anthocyanidins as substrate but not flavonols. Also, UDP-galactose was used preferentially as the sugar donor. These results indicate AcUFGT3a is the key enzyme regulating anthocyanin accumulation in red-fleshed kiwifruit.

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Section: Resultsmentioning

confidence: 99%

Biochemical and functional characterization of AcUFGT3a, a galactosyltransferase involved in anthocyanin biosynthesis in the red‐fleshed kiwifruit (Actinidia chinensis)

Liu

Zhou

et al. 2017

Physiologia Plantarum

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“…, 2003). F3GT1 showed 67% identity to the predicted translation of the GaT gene of Aralia cordata ( AcGaT ) (Nagashima et al. , 2004) and 60.9% identity to the predicted translation of the F3GaT gene from Petunia hybrida ( PhF3GaT ) (Miller et al.…”

Section: Resultsmentioning

confidence: 99%

Identification and characterisation of F3GT1 and F3GGT1, two glycosyltransferases responsible for anthocyanin biosynthesis in red‐fleshed kiwifruit (Actinidia chinensis)

Montefiori¹,

Espley²,

Stevenson³

et al. 2010

The Plant Journal

177

149

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Much of the diversity of anthocyanins is due to the action of glycosyltransferases, which add sugar moieties to anthocyanidins. We identified two glycosyltransferases, F3GT1 and F3GGT1, from red-fleshed kiwifruit (Actinidia chinensis) that perform sequential glycosylation steps. Red-fleshed genotypes of kiwifruit accumulate anthocyanins mainly in the form of cyanidin 3-O-xylo-galactoside. Genes in the anthocyanin and flavonoid biosynthetic pathway were identified and shown to be expressed in fruit tissue. However, only the expression of the glycosyltransferase F3GT1 was correlated with anthocyanin accumulation in red tissues. Recombinant enzyme assays in vitro and in vivo RNA interference (RNAi) demonstrated the role of F3GT1 in the production of cyanidin 3-O-galactoside. F3GGT1 was shown to further glycosylate the sugar moiety of the anthocyanins. This second glycosylation can affect the solubility and stability of the pigments and modify their colour. We show that recombinant F3GGT1 can catalyse the addition of UDP-xylose to cyanidin 3-galactoside. While F3GGT1 is responsible for the end-product of the pathway, F3GT1 is likely to be the key enzyme regulating the accumulation of anthocyanin in red-fleshed kiwifruit varieties.

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“…An unrooted phylogenetic tree was generated (Fig. 5 a), which showed that DkFGT belongs to a subclade F (Bowles et al 2005 ; Gachon et al 2005 ) with seven other sequences, six of which have already been shown to have anthocyanidin/flavonoid 3 -O- GT activities, i.e., flavonol 3 -O- rhamnosyltransferase (UGT78D1; Jones et al 2003 ), flavonoid 3 -O- GlcT (UGT78D2; Tohge et al 2005 ) and flavonoid 3- O -arabinosyltransferase (UGT78D3; Yonekura-Sakakibara et al 2008 ) from A. thaliana (Tohge et al 2005 ), and three flavonoid 3 -O- galactosyltransferase (F3GalT) from petunia (Miller et al 1999 ), Aralia cordata (Nagashima et al 2004 ), and Vigna mungo L. (Mato et al 1998 ). This group is distinct from group E, which includes the enzyme involved in PA accumulation, UGT72L1 (Pang et al 2008 ), and group L, which contains FGTs with less strict region-specificity (Tian et al 2006 ).…”

Section: Resultsmentioning

confidence: 99%

Molecular identification of 1-Cys peroxiredoxin and anthocyanidin/flavonol 3-O-galactosyltransferase from proanthocyanidin-rich young fruits of persimmon (Diospyros kaki Thunb.)

Ikegami

Akagi

Potter

et al. 2009

Planta

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Fruits of persimmon (Diospyros kaki Thunb.) accumulate large amounts of proanthocyanidins (PAs) in the early stages of development. Astringent (A)-type fruits remain rich in soluble PAs even after they reach full-mature stage, whereas non-astringent (NA)-type fruits lose these compounds before full maturation. As a Wrst step to elucidate the mechanism of PA accumulation in this non-model species, we used suppression subtractive hybridization to identify transcripts accumulating diVerently in young fruits of A-and NA-type. Interestingly, only a few clones involved in PA biosynthesis were identiWed in A-NA libraries. Represented by multiple clones were those encoding a novel 1-Cys peroxiredoxin and a new member of family 1 glycosyltransferases. Quantitative RT-PCR analyses conWrmed correlation of the amount of PAs and accumulation of transcripts encoding these proteins in young persimmon fruits. Furthermore, the new family 1 glycosyltransferase was produced in Escherichia coli and shown to eYciently catalyze galactosylation at 3-hydroxyl groups of several anthocyanidins and Xavonols. These Wndings suggest a complex mechanism of PA accumulation in persimmon fruits.

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Anthocyanin galactosyltransferase from Aralia cordata, cDNA cloning and characterization

Cited by 10 publications

References 15 publications

Biochemical and functional characterization of AcUFGT3a, a galactosyltransferase involved in anthocyanin biosynthesis in the red‐fleshed kiwifruit (Actinidia chinensis)

Biochemical and functional characterization of AcUFGT3a, a galactosyltransferase involved in anthocyanin biosynthesis in the red‐fleshed kiwifruit (Actinidia chinensis)

Identification and characterisation of F3GT1 and F3GGT1, two glycosyltransferases responsible for anthocyanin biosynthesis in red‐fleshed kiwifruit (Actinidia chinensis)

Molecular identification of 1-Cys peroxiredoxin and anthocyanidin/flavonol 3-O-galactosyltransferase from proanthocyanidin-rich young fruits of persimmon (Diospyros kaki Thunb.)

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