Anthocyanin improves kidney function in diabetic kidney disease by regulating amino acid metabolism

Abstract

Background Diabetic kidney disease (DKD) is among the most important causes for chronic kidney disease. Anthocyanins (ANT) are polyphenolic compounds present in various food and play an important role in ameliorating hyperglycemia and insulin sensitivity. However, the effects of ANT in DKD are still poorly understood. This study aimed to investigate the effect of ANT (cyanidin-3-O-glucoside [C3G]) on the renal function of DKD, and whether the anti-DKD effect of ANT is related to metabolic pathways. Methods To explore the role of ANT in DKD, we performed the examination of blood glucose, renal function, and histopathology. As for the mechanism, we designed the label-free quantification proteomics and nontargeted metabolomics analysis for kidney and serum. Subsequently, we revealed the anti-DKD effect of ANT through the bioinformatic analysis. Results We showed that the fasting blood glucose level (− 6.1 mmol/L, P = 0.037), perimeter of glomerular lesions (− 24.1 μm, P = 0.030), fibrosis score of glomerular (− 8.8%, P = 0.002), and kidney function (Cystatin C: − 701.4 pg/mL, P = 0.043; urine creatinine: − 701.4 mmol/L, P = 0.032) were significantly alleviated in DKD mice after ANT treatment compared to untreated in the 20th week. Further, proteins and metabolites in the kidneys of DKD mice were observed to be dramatically altered due to changes in amino acid metabolism with ANT treatment; mainly, taurine and hypotaurine metabolism pathway was upregulated (P = 0.0001, t value = 5.97). Furthermore, upregulated tryptophan metabolism (P < 0.0001, t value = 5.94) and tyrosine metabolism (P = 0.0037, t value = 2.91) pathways had effects on serum of DKD mice as responsed ANT regulating. Conclusions Our results suggested that prevention of the progression of DKD by ANT could be related to the regulation of amino acid metabolism. The use of dietary ANT may be one of the dietary strategies to prevent and treat DKD.

Untargeted serum metabolomics analysis of Trichinella spiralis-infected mouse

“…spiralis infection, we performed pathway analysis with metabolomic data alone, as well as metabolomic data from this study integrated with proteomic data from a previous study by our group [ 37 ]. Changes in level of metabolites and proteins would fundamentally affect pathways and lead to impaired functions, reflecting molecular mechanism in the organism [ 53 , 54 ]. Integration of proteomic to metabolomic data increased the number and confidence of the identified pathways dramatically.…”

Insights Into the Functional Mechanism of Diabetic Kidney Disease Treatment with Sinensetin Based on Network Pharmacology and Molecular Docking

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Clinical efficacy and safety of Xuefu Zhuyu decoction in the treatment of diabetic kidney disease: A protocol for systematic review and meta-analysis

“…However, Traditional Chinese medicine has a significant effect on the treatment of chronic kidney disease, and some experts suggest that using triptolide and emodin can improve the treatment of proteinuria in the treatment of chronic kidney disease, [16,17] traditional Chinese medicine ingredients such as ginsenosides, tanshinone IIA, anthocyanin also have beneficial effects on DKD. [18][19][20] Xuefu Zhuyu decoction, as the first prescription of promoting blood circulation and removing blood stasis, it has more clinical RCTs for DKD, especially in China. However, there is no systematic review comprehensively describing the effectiveness and safety of Xuefu Zhuyu decoction for DKD.…”