Anthocyanin production in callus cultures of Cleome rosea: Modulation by culture conditions and characterization of pigments by means of HPLC-DAD/ESIMS

Simões, Cláudia Maria Oliveira; Bizarri, Carlos Henrique Brasil; Cordeiro, Lívia da Silva; Castro, Tatiana Carvalho de; Coutada, Leonardo César Machado; Silva, Antônio J. R. da; Albarello, Norma; Mansur, Elisabeth

doi:10.1016/j.plaphy.2009.06.005

Cited by 68 publications

(37 citation statements)

References 34 publications

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“…Similar response to reduced NH 4 ? nitrogen was also reported by Simões et al (2009), who postulated that the increase in anthocyanin content in callus culture of Cleome rosea was probably due to nutritional stress induced by reduction of salt concentration to quarter strength. a 4%, b 5% (control), c 6%, d 7% sucrose.…”

Section: Effects Of Nhsupporting

confidence: 54%

“…to NO 3 -nitrogen concentration) were different. Such media preference could be due to a shift of cells from growth state to metabolite production state, as suggested by Simões et al (2009).…”

Section: Callus Inductionmentioning

confidence: 94%

“…(Chi and Cormier 1991), Oxalis linearis (Meyer and Staden 1995), Oxalis reclinata (Makunga et al 1997), Platanus acerifolia (Alami and Clerivet 2000), Daucus carota (Sudha and Ravishankar 2003), Fragaria ananassa (Asano et al 2002;Edahiro et al 2005), Ipomoea batatas (Nishiyama and Yamakawa 2004), Raphanus sativus (Betsui et al 2004), rose (Hennayake et al 2006), Torenia fourunieri (Nagira et al 2006) and Cleome rosea (Simões et al 2009). However, research on in vitro induction of anthocyanin pigments from flower crops, especially roses, using solid callus cultures is non-existent to our knowledge.…”

Section: Introductionmentioning

confidence: 99%

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Induction of anthocyanin pigments in callus cultures of Rosa hybrida L. in response to sucrose and ammonical nitrogen levels

Ram

Prasad

Kaur

et al. 2010

Plant Cell Tiss Organ Cult

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The influence of varied concentrations of sucrose and ammonical (NH 4 ? ) nitrogen on in vitro induction and expression of anthocyanin pigments from Rosa hybrida cv. 'Pusa Ajay' was investigated. Of two explants (petal and leaf discs) selected and cultured under two different conditions (light and dark), leaf discs were found to be most suitable for callus initiation. Profuse and early callus induction was observed when leaf discs of rose were cultured under total dark conditions on solid Murashige and Skoog (MS) medium supplemented with 4.0 mg l -1 2,4-dichlorophenoxyacetic acid (2,4-D). Early pigment initiation, enhancement and maximum anthocyanin production from calluses were recorded when leaf discs were cultured on Euphorbia millii (EM) medium supplemented with 7% sucrose compared with calluses cultured at 4% sucrose concentration under 16/8 h (light/dark) photoperiod regime. Reducing the concentration of NH 4 ? nitrogen in the solid MS medium led to slight improvement in anthocyanin production in rose leaf calluses.

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Section: Effects Of Nhsupporting

confidence: 54%

Section: Callus Inductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Induction of anthocyanin pigments in callus cultures of Rosa hybrida L. in response to sucrose and ammonical nitrogen levels

Ram

Prasad

Kaur

et al. 2010

Plant Cell Tiss Organ Cult

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“…Wang et al demonstrated that shading effectively improved tea quality by altering the expression of flavonoid pathway genes in tea leaves, changing the flavonoid content . Moreover, in the callus of Cleome rosea, increasing the light intensity significantly increased the anthocyanin content (Simoes et al 2009). The photoperiod, an important light factor, also regulates the biosynthesis of secondary metabolites in plants (Jaakola and Hohtola 2010).…”

Section: Introductionmentioning

confidence: 97%

Photoperiod and shading regulate coloration and anthocyanin accumulation in the leaves of malus crabapples

Zhang

Meng

et al. 2015

Plant Cell Tiss Organ Cult

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In many ornamental plants, anthocyanin accumulation in leaves is a critical determinant of foliage coloration. However, the mechanism by which photoperiod and shading affect coloration and anthocyanin accumulation in the leaves of woody plants is less clear. By examining the leaves and calli of crabapple (Malus spp.) cultivars with different leaf color characteristics, we analyzed the foliage coloration, flavonoid contents, and expression levels of anthocyanin biosynthetic genes and the McMYB10 transcription factor under different photoperiods and shading treatments. The red color parameters, flavonoid contents, and expression levels of some anthocyaninrelated genes in both the leaf and callus were generally higher under sun-exposure or long-day treatments compared to shading or short-day treatments. These data indicate that red color formation in cultivars with leaves that are naturally red requires a suitable photoperiod and light intensity; high light intensity or long-day conditions activate anthocyanin biosynthesis-related genes, leading to anthocyanin accumulation. Our results also indicate that the regulation of anthocyanin biosynthetic metabolism in response to light conditions differs between red-leafed and green-leafed cultivars. Taken together, our data reveal that photoperiod and light intensity are important in the regulation of pigmentation and anthocyanin accumulation in crabapple leaves and calli. Moreover, we provide a useful approach for studying and inducing the production of flavonoids by crabapple callus culture under certain photoperiods.

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“…Their production in spontaneous plants is very limited by numerous problems such as the fluctuations of environmental factors and the abiotic constraints, difficult cultivation, deficient abundance (Matkowski 2008). Actually, the plant cell and tissue culture become an alternative potential means for the production and the accumulation of many valuable secondary metabolites because it can provide several benefits to minimize these problems allowing the continuous, reliable, and predictable production of these molecules independently of seasonal variations as well as the optimization and the enhancement of their biosynthesis under well controlled conditions (Simoés et al 2009). …”

Section: Introductionmentioning

confidence: 99%

Effects of 2,4-dichlorophenoxyacetic acid combined to 6-Benzylaminopurine on callus induction, total phenolic and ascorbic acid production, and antioxidant activities in leaf tissue cultures of Crataegus azarolus L. var. aronia

et al. 2015

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The present research work describes the effects of 2,4-dichlorophenoxyacetic acid (2,4-D)/Ben-zylaminopurine (BAP) ratio on callus induction, total phenolic and ascorbic acid production, and antioxidant activities in leafderived calli of Crataegus azarolus (hawthorn). The supplementation of 1.0 mg/L 2,4-D and 1.0 mg/L BAP to MS medium was found to be the most efficient for callus induction (as percentage and fresh weigh). The results of biochemical analysis showed that the highest total phenolic contents were obtained in callus cultured on MS medium supplemented with 2.0 mg/L 2,4-D and 1.0 mg/L BAP (52 ± 0.56 mg GAE/g DM) and were significantly lower than those of intact leaves (76±1.72mg GAE/g DM). However, the highest ascorbic acid contents were found in callus cultured on MS medium supplemented with 1.0 mg/L 2,4-D and 0.5 mg/L BAP (0.96 ± 0.13 mg AAE/g DM) and these amounts were statistically similar to those found in leaf tissues (0.74 ± 0.07 mg AAE/g DM). Antioxidant activities of callus extracts were determined using two TEAC assays and results showed that extract of callus cultured on MS medium supplemented with 2.0 mg/L 2,4-D and 1.0 mg/L BAP have the greatest antiradical activities against DPPH (124 ± 2.92 mg TE/g DM) and ABTS (0.19 ± 0.02 mg TE/g DM) compared to the leaves of field-grown plant. Thus, the use of high level of 2,4-D over BAP can be suitable to enhance the quality more than the quantity of bioactive compounds in leaf callus culture of hawthorn.

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Anthocyanin production in callus cultures of Cleome rosea: Modulation by culture conditions and characterization of pigments by means of HPLC-DAD/ESIMS

Cited by 68 publications

References 34 publications

Induction of anthocyanin pigments in callus cultures of Rosa hybrida L. in response to sucrose and ammonical nitrogen levels

Induction of anthocyanin pigments in callus cultures of Rosa hybrida L. in response to sucrose and ammonical nitrogen levels

Photoperiod and shading regulate coloration and anthocyanin accumulation in the leaves of malus crabapples

Effects of 2,4-dichlorophenoxyacetic acid combined to 6-Benzylaminopurine on callus induction, total phenolic and ascorbic acid production, and antioxidant activities in leaf tissue cultures of Crataegus azarolus L. var. aronia

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