Anthocyanin profiles in south Patagonian wild berries by HPLC-DAD-ESI-MS/MS

Ruíz, Antonieta; Hermosín‐Gutiérrez, Isidro; Vergara, Carola; Baer, Dietrich von; Zapata, Melcy; Hitschfeld, Antonieta; Obando, Luis Alberto Valverde; Mardones, Claudia

doi:10.1016/j.foodres.2013.01.043

Cited by 113 publications

(110 citation statements)

References 28 publications

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“…[23][24][25] In the case of calafate extract, the reported anthocyanin pattern agrees with the one previously found. 10,26 Finally, in blueberry extracts, the compounds observed are in accordance with those reported before, 21,27 with malvidin-3-glucoside, malvidin-3-galactoside, malvidin-3-arabinoside, and delphinidin-3-galactoside as major components. Thus, the identification of anthocyanins by LC-MS in the three extracts studied was consistent with previous reports by other authors.…”

Section: It Has Been Recognized That Increased Body Adiposity Is Habisupporting

confidence: 89%

Chilean Native Fruit Extracts Inhibit Inflammation Linked to the Pathogenic Interaction Between Adipocytes and Macrophages

Reyes-Farías

Vasquez

Ovalle-Marin

et al. 2015

Journal of Medicinal Food

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Obesity is characterized by an increase in the infiltration of monocytes into the adipose tissue, causing an inflammatory condition associated with, for example, the development of insulin resistance. Thus, anti-inflammatory-based treatments could emerge as a novel and interesting approach. It has been reported that Chilean native fruits maqui (Aristotelia chilensis) and calafate (Berberis microphylla) present high contents of polyphenols, which are known for their antioxidant and anti-inflammatory properties. The aim of this study was to evaluate the ability of extracts of these fruits to block the pathogenic interaction between adipocytes and macrophages in vitro and to compare its effect with blueberry (Vaccinium corymbosum) extract treatment, which has been already described to possess several biomedical benefits. RAW264.7 macrophages were treated with 5 μg/mL lipopolysaccharides (LPS), with conditioned media (CM) from fully differentiated 3T3-L1 adipocytes, or in a coculture (CC) with 3T3-L1 adipocytes, in the presence or absence of 100 μM [total polyphenolic content] of each extract for 24 h. The gene expression and secretion profile of several inflammatory markers were evaluated. Nitric oxide secretion induced by LPS, CM, and CC was reduced by the presence of maqui (-12.2%, -45.6%, and -14.7%, respectively) and calafate (-27.6%, -43.9%, and -11.8%, respectively) extracts. Gene expression of inducible nitric oxide synthase and TNF-α was inhibited and of IL-10 was induced by maqui and calafate extract incubation. In conclusion, the extracts of these fruits present important inhibitory-like features over the inflammatory response of the interaction between adipocytes and macrophages, comprising a potential therapeutic tool against comorbidities associated with obesity development.

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Section: It Has Been Recognized That Increased Body Adiposity Is Habisupporting

confidence: 89%

Chilean Native Fruit Extracts Inhibit Inflammation Linked to the Pathogenic Interaction Between Adipocytes and Macrophages

Reyes-Farías

Vasquez

Ovalle-Marin

et al. 2015

Journal of Medicinal Food

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“…For the optimization of separation conditions, mobile phases, gradient, and flow were optimized based on those previously reported for Chilean berries by Ruiz et al . where a Kromasil 100‐5‐C 18 (4.6 × 250 mm, 5 μm) column was used.…”

Section: Resultsmentioning

confidence: 99%

“…Stationary phases with a lower particle size have been widely studied due to diminution in eddy diffusion coefficients in the van Deemter equation, a decreased axial diffusion by diminution of intraparticle porosity, and a reduced mass transference by decreasing diffusion into the particles of the stationary phase, allowing for a reduction in the total analysis time in comparison with conventional HPLC chromatography. After optimization of the chromatographic separation conditions, the strawberry extracts were analyzed more quickly and efficiently than in the previously used method, where a total analysis time of 50 min was required. Similar results have been previously reported for the separation of flavonols from Berberis microphylla fruits, where the total analysis time was reduced from 30 to 15 min …”

Section: Resultsmentioning

confidence: 99%

“…An extraction methodology using a methanol and formic acid mixture and a chromatographic separation method High Performance Liquid Chromatography with Diode Array Detector (HPLC‐DAD) for the determination of phenolic compounds, mainly of anthocyanins and flavonols, in strawberry fruits was developed based on the procedure reported by Ruiz et al . through the use of a Kromasil ClassicShell‐2.5‐C 18 (4.6 × 100 mm, 2.5 μm) column.…”

Section: Methodsmentioning

confidence: 99%

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Effect of fertilization and arbuscular mycorrhizal fungal inoculation on antioxidant profiles and activities in Fragaria ananassa fruit

et al. 2018

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“…Four major anthocyanidins were identified and quantified from the crude extracts according to the method by Ruiz et al (2013) with modifications. A Shimadzu HPLC system equipped with a diode array detector, column heater and automatic injection.…”

Section: Identification and Quantificationmentioning

confidence: 99%

Stability and degradation kinetics of crude anthocyanin extracts from H. sabdariffa

2017

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Hibiscus sabdariffa is an under-utilized plant that has reported to have great potential in the food industry. The vibrant red pigment from the calyces indicate a source of anthocyanins. Anthocyanins would make ideal natural food colourants with additional nutritional benefits however stability is a hindering factor. Stability studies were the main focus of this study. Crude anthocyanins were extracted using four different solvent systems. The crude extracts were analysed under the following parameters; heat (50 and 80 °C), light (darkness and 20 W light) and pH (pH 1-9) stability. Degradation kinetic studies were done on thermally treated samples. Radical scavenging ability was thereafter calculated. Anthocyanidins were identified and quantified by HPLC coupled with a Diode Array Detector (DAD). Total phenolic content was determined with Folin -Ciocalteu's method. Approximately 87% of pigments were retained when heated at 50 °C while heating at 80 °C resulted in 61% pigment retention. The pH stability of samples incubated for 7 days indicated that crude anthocyanins degraded slower at acidic pH. Light stability showed slower degradation in dark incubated samples resulting in 84% pigment retention after a 10 day period. H. sabdariffa shows potential for the application of a food in products such as jelly and yoghurt.

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Anthocyanin profiles in south Patagonian wild berries by HPLC-DAD-ESI-MS/MS

Cited by 113 publications

References 28 publications

Chilean Native Fruit Extracts Inhibit Inflammation Linked to the Pathogenic Interaction Between Adipocytes and Macrophages

Chilean Native Fruit Extracts Inhibit Inflammation Linked to the Pathogenic Interaction Between Adipocytes and Macrophages

Effect of fertilization and arbuscular mycorrhizal fungal inoculation on antioxidant profiles and activities in Fragaria ananassa fruit

Stability and degradation kinetics of crude anthocyanin extracts from H. sabdariffa

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