Anthocyanins from <i>Aronia melanocarpa</i> Bound to Amylopectin Nanoparticles: Tissue Distribution and In Vivo Oxidative Damage Protection

Liu

Chemistry & Biodiversity

et al. 2023

Bletilla striata (Thunb.) Reichb.f. is a perennial herb with abundant active ingredients. Previous research mainly focused on its tubers, however, the study on flowers, especially the variation of active ingredient contents at different flowering stages, was rarely seen. This study analyzed the total phenols, flavonoids, polysaccharides, anthocyanins, and cyanidin‐3‐O‐glucoside content of B. striata flowers which were in cultivated in Herb Garden of Zhejiang A&F University and collected in May, 2019, in order to investigate the changes in active ingredients and antioxidant capacity among different flowering stages (bud, initial, and full bloom). Changes in radical scavenging capability of DPPH (1,1‐Diphenyl‐2‐picrylhydrazyl radical), ABTS (2,2′‐azinobis(3‐ethylbenzthiazoline‐6‐sulphonate)), and hydroxy were analyzed. Significant differences in active ingredient content of flowers were detected among different flowering stages. The total phenolic content increased continuously during the entire flowering stage. The contents of total flavonoid, total polysaccharide, and cyanidin‐3‐O‐glucoside reached peaks at the initial blooming stage and then fell as the flowering process continued. The antioxidant activity in initial stage was the highest than in any other flowering stages. Therefore, we conclude that the initial blooming stage is the best harvesting stage of B. striata flowers. This study provides a robust basis for the harvest and utilization of B. striata flowers in food, medical, and cosmetic industries.

Section: Resultsmentioning

confidence: 99%

Section: 2)mentioning

confidence: 99%

Effect of Flowering Stages on the Content of Active Ingredients and Antioxidant Capability of Bletilla striata Flowers

Liu

Chemistry & Biodiversity

et al. 2023

“…In Wistar rats, the administration of anthocyanins led to a dose-dependent improvement in the activity of serum antioxidant enzymes such as catalase, superoxide dismutase and glutathione peroxidase [ 182 ]. Furthermore, the nano-encapsulation of anthocyanins increased their stability and bioavailability in various tissues, improving protection against oxidative damage in in vivo studies [ 190 ]. In another study in guinea pigs fed a high-cholesterol diet fortified with an 8% addition of fresh blueberries for 75 days, a reduction in oxidative stress was observed by reducing malondialdehyde concentrations [ 183 ].…”

Section: The Effects Of Anthocyanins On the Prevention And Supportive...mentioning

confidence: 99%

Effects of Anthocyanins on Components of Metabolic Syndrome—A Review

Godyla-Jabłoński,

Raczkowska,

Jodkowska

et al. 2024

Nutrients

Metabolic syndrome (MetS) is a significant health problem. The co-occurrence of obesity, carbohydrate metabolism disorders, hypertension and atherogenic dyslipidaemia is estimated to affect 20–30% of adults worldwide. Researchers are seeking solutions to prevent and treat the conditions related to MetS. Preventive medicine, which focuses on modifiable cardiovascular risk factors, including diet, plays a special role. A diet rich in fruits and vegetables has documented health benefits, mainly due to the polyphenolic compounds it contains. Anthocyanins represent a major group of polyphenols; they exhibit anti-atherosclerotic, antihypertensive, antithrombotic, anti-inflammatory and anticancer activities, as well as beneficial effects on endothelial function and oxidative stress. This review presents recent reports on the mechanisms involved in the protective effects of anthocyanins on the body, especially among people with MetS. It includes epidemiological data, in vivo and in vitro preclinical studies and clinical observational studies. Anthocyanins are effective, widely available compounds that can be used in both the prevention and treatment of MetS and its complications. Increased consumption of anthocyanin-rich foods may contribute to the maintenance of normal body weight and modulation of the lipid profile in adults. However, further investigation is needed to confirm the beneficial effects of anthocyanins on serum glucose levels, improvement in insulin sensitivity and reduction in systolic and diastolic blood pressure.

“…Anthocyanin, as active molecules widely present in dark-colored fruits and vegetables, are flavonoid structures with polyphenol substitution. , Anthocyanins have been reported to possess a variety of bioactive properties, including antioxidant, antidiabetic, anti-inflammatory, and antitumor activities. Despite extensive research on the regulatory pathways of anthocyanins, , the precise molecular mechanisms and specific targets of their actions remain to be fully elucidated.…”

Section: Introductionmentioning

confidence: 99%

Covalent Interactions of Anthocyanins with Proteins: Activity-Based Protein Profiling of Cyanidin-3-O-glucoside

Hu,

Yu,

Huang

et al. 2024

J. Agric. Food Chem.

Anthocyanins are common natural pigments with a variety of physiological activities. Traditional perspectives attribute their molecular mechanism to noncovalent interactions influencing signaling pathways. However, this ignores the nature of its benzopyrylium skeleton, which readily reacts with the electron-rich groups of proteins. Here, we modified cyanidin-3-O-glucoside (C3G) via activity-based protein profiling technology by our previous synthesis route and prepared the covalent binding probe (C3G-Probe) and the noncovalent photoaffinity probe (C3G-Diazirine). The properties of C3G′s covalent binding to proteins were also discovered by comparing the labeling of the two probes to the whole HepG2 cell proteome. We further explored its target proteins and enriched pathways in HepG2 and HeLa cells. Western blot analysis further confirmed the covalent binding of C3G to four target proteins: insulin-degrading enzyme, metal cation symporter ZIP14, spermatid perinuclear RNA-binding protein, and Cystatin-B. Pathway analysis showed that covalent targets of C3G were concentrated in metabolic pathways and several ribonucleoprotein complexes that were also coenriched. The results of this study provide new insights into the interaction of the naturally active molecule C3G with proteins.