Anthocyanins from purple sweet potato alleviate doxorubicin‐induced cardiotoxicity in vitro and in vivo

Abstract

In this study, anthocyanins were extracted and purified from purple sweet potato anthocyanins (PSPA) and the alleviative effect of PSPA on doxorubicin (DOX)‐induced cardiotoxicity was investigated. High Performance Liquid Chromatography‐Mass Spectrometry (HPLC‐MS) results showed that 10 kinds of substances were identified in PSPA and the PSPA was mainly composed of cyanidin (62.9%) and peonidin (21.46%). In in vitro experiments, PSPA reduced the excessive release of inflammatory factors (NO and TNF‐α) induced by DOX and decreased the secretion of trimethylamine oxide (TMAO), lactic dehydrogenase (LDH), and creatine kinase (CK) caused by myocardial injury. In in vivo experiments, PSPA inhibited the release of NO and MDA levels in heart tissue. Meanwhile, mice treated with PSPA decreased the levels of LDH, CK, TNF‐α, and TMAO in serum and heart tissue when compared with the DOX group. In addition, the histopathological results of the heart tissue also showed a protective effect of PSPA on the pathological changes in heart. These results provide a reference for the application of PSPA as a functional food to intervene in DOX‐induced cardiotoxicity. Practical applications The effects of anthocyanins from purple sweet potato anthocyanins (PSPA) on doxorubicin (DOX)‐induced cardiotoxicity were investigated in vitro and in vivo. The results indicated that PSPA could significantly ameliorate DOX‐induced heart failure. The obtained results could provide the potential application of PSPA as an alternative therapy for cardiotoxicity caused by DOX in the functional food industry.

Phenotyping the Anthocyanin Content of Various Organs in Purple Corn Using a Digital Camera

“…Anthocyanins are widely used in cosmetics and in food colorings in industry [60][61][62][63]. Numerous studies have shown that anthocyanins have very good medicinal value and significant health effects, such as anti-cancer, anti-inflammation, anti-aging, anti-obesity and protection of vision [64][65][66][67][68]. A a considerable supply of anthocyanins is required for industry use.…”

Combination Anticancer Therapies Using Selected Phytochemicals

“…Anthocyanin from purple sweet potato had decreased doxorubicin cardiac toxicity using different concentrations (100, 200, and 400 μg/mL) according to in vitro and in vivo study. The previously mentioned effect was due to the decrease in inflammatory factors, such as nitric oxide and TNF-α, also due to the decline in creatine kinase, trimethylamine oxide, and lactic dehydrogenase triggered by myocardial damage [ 293 ]. Moreover, with 20 μg/mL trastuzumab, 1 μg/mL anthocyanins cyanidin 3 glucoside proved to show a synergistic effect in vitro and in vivo.…”

Purple sweet potato anthocyanins normalize the blood glucose concentration and restore the gut microbiota in mice with type 2 diabetes mellitus

“…The high acylated anthocyanin content provides color and greater stability and is directly absorbed into the bloodstream [ 5 , 6 ]. Previous studies have suggested that PSPA shows a plethora of biological activities, including antioxidant [ 7 ], anti-inflammatory [ 8 ] anticancer [ 9 ], and cardiovascular protective functions [ 10 ]. Many of these health benefits are attributed to the potent antioxidant activity of anthocyanins, which can protect DNA, proteins, and lipids from damage and activate specific detoxification enzymes, such as glutathione reductase, glutathione peroxidase, and glutathione S-transferase, to reduce oxidative stress [ 11 ].…”