Anthocyanins in the Bracts of <i>Curcuma</i> Species and Relationship of the Species Based on Anthocyanin Composition

Koshioka, Masaji; Umegaki, Naoko; Boontiang, Kriangsuk; Pornchuti, W.; Thammasiri, K.; Tatsuzawa, Fumi; Nakayama, Masayoshi; Tateishi, Akira; Kubota, Shin

doi:10.1177/1934578x1501000320

Cited by 7 publications

(5 citation statements)

References 8 publications

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“…The CUAL08G6149 was also the only one of these nine genes that was aggregated into the “pink” module with the highest correlation to anthocyanin accumulation in the WGCNA analysis ( Supplementary Table 10 ). It was reported that malvidin 3-rutinoside was the major anthocyanin type in distal bracts of C. alismatifolia ( Nakayama et al, 2000 ; Koshioka et al, 2015 ). As illustrated in Figure 3A , F3′5′H is a specific catalyzing enzyme in the malvidin biosynthesis process.…”

Section: Discussionmentioning

confidence: 99%

“…The prominent elliptical distal bracts are generally varying degrees of pink with green tips, which determine the attractiveness of the inflorescences. It has been reported that the color of the distal bracts is determined by the accumulation of anthocyanin pigments ( Nakayama et al, 2000 ; Koshioka et al, 2015 ). Anthocyanins are a class of plant flavonoid secondary metabolites, which are helpful not only in attracting pollinators and facilitating seed dispersion, but also play key roles in biotic and abiotic stress responses ( Saigo et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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The Chromosome-Scale Assembly of the Curcuma alismatifolia Genome Provides Insight Into Anthocyanin and Terpenoid Biosynthesis

Dong¹,

Zou²,

Mao³

et al. 2022

Front. Plant Sci.

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Curcuma alismatifolia, a bulbous flower known for its showy bracts, is widely used around the world as a cut flower, potted, and garden plant. Besides its ornamental value, this species is rich in terpenoid metabolites and could serve as a resource for essential oils. Here, we report a chromosome-level genome assembly of C. alismatifolia and describe its biosynthetic pathways for anthocyanins and terpenoids. This high-quality, assembled genome size is 991.3 Mb with a scaffold N50 value of 56.7 Mb. Evolutionary analysis of the genome suggests that C. alismatifolia diverged from Zingiber officinale about 9.7 million years ago, after it underwent a whole-genome duplication. Transcriptome analysis was performed on bracts at five developmental stages. Nine highly expressed genes were identified, encoding for six enzymes downstream of the anthocyanin biosynthetic pathway. Of these, one gene encoding F3′5′H might be a key node in the regulation of bract color formation. Co-expression network analysis showed that MYB, bHLH, NAC, and ERF transcription factors collectively regulated color formation in the bracts. Characterization of terpenoid biosynthesis genes revealed their dispersal and tandem duplications, both of which contributed greatly to the increase in the number of terpene synthase genes in C. alismatifolia, especially to species-specific expansion of sesquiterpene synthase genes. This work facilitates understanding of genetic basis of anthocyanin and terpenoid biosynthesis and could accelerate the selective breeding of C. alismatifolia varieties with higher ornamental and medicinal value.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Chromosome-Scale Assembly of the Curcuma alismatifolia Genome Provides Insight Into Anthocyanin and Terpenoid Biosynthesis

Dong¹,

Zou²,

Mao³

et al. 2022

Front. Plant Sci.

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“…Among the many cultivars of Siam tulip, “Chiang Mai Pink” is the most popular variety with broad market prospects (Fukai and Udomdee 2005 ; Lu 2007 ; Mao et al 2018 ; Taheri et al 2012 , 2014 ). At present, studies on the traits of C. alismatifolia have mainly focused on bract color (Koshioka et al 2015 ), vase life (Kjonboon and Kanlayanarat 2005 ), inflorescence and flower initiation and development (Fukai and Udomdee 2005 ). As the most important ornamental trait of C. alismatifolia , bract color (Fig.…”

Section: Introductionmentioning

confidence: 99%

The genomic and bulked segregant analysis of Curcuma alismatifolia revealed its diverse bract pigmentation

Liao

Zhang

et al. 2022

aBIOTECH

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Compared with most flowers where the showy part comprises specialized leaves (petals) directly subtending the reproductive structures, most Zingiberaceae species produce showy “flowers” through modifications of leaves (bracts) subtending the true flowers throughout an inflorescence. Curcuma alismatifolia, belonging to the Zingiberaceae family, a plant species originating from Southeast Asia, has become increasingly popular in the flower market worldwide because of its varied and esthetically pleasing bracts produced in different cultivars. Here, we present the chromosome-scale genome assembly of C. alismatifolia “Chiang Mai Pink” and explore the underlying mechanisms of bract pigmentation. Comparative genomic analysis revealed C. alismatifolia contains a residual signal of whole-genome duplication. Duplicated genes, including pigment-related genes, exhibit functional and structural differentiation resulting in diverse bract colors among C. alismatifolia cultivars. In addition, we identified the key genes that produce different colored bracts in C. alismatifolia, such as F3′5'H, DFR, ANS and several transcription factors for anthocyanin synthesis, as well as chlH and CAO in the chlorophyll synthesis pathway by conducting transcriptomic analysis, bulked segregant analysis using both DNA and RNA data, and population genomic analysis. This work provides data for understanding the mechanism of bract pigmentation and will accelerate breeding in developing novel cultivars with richly colored bracts in C. alismatifolia and related species. It is also important to understand the variation in the evolution of the Zingiberaceae family.

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“…Koshioka sorted the systematic relationships of nine species of Curcuma into two categories according to 11 kinds of anthocyanins they possessed (Koshioka et al 2015). The results showed that both parents and hybrids as well as Lonicera korolkowii , Lonicera ruprechtiana and Lonicera tatarica are clustered in one group, which is partly consistent with the morphological taxonomy of Lonicera , indicating that the floral pigment components have a certain scientific value for the classification of Lonicera.…”

Section: Discussionmentioning

confidence: 99%

A taxonomic study on pollen morphology, karyotype features, floral and fruit pigment components of 13 taxa of Lonicera L.

Zhai²,

Sun

et al. 2023

Palynology

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Due to extensive introduction and breeding, the taxonomic relationships of Lonicera L. are unclear. Alternative scientific methods are needed to explore the classification of these new Lonicera. In this paper, the pollen morphology characteristics, karyotype features, and floral and fruit pigment components of 13 taxa of Lonicera were analyzed by means of scanning electron microscopy, karyotype analysis, High Performance Liquid Chromatography Mass Spectrometry (HPLC-MS) and Gas Chromatography-Mass Spectrometry (GC-MS). The 13 representatives of Lonicera were divided into groups of four, three, three and three taxa through the analysis of pollen morphology characteristics, karyotype features, floral composition and fruit pigment composition, respectively. Lonicera ruprechtiana and Lonicera tatarica always clustered together. Five hybrids, including Lonicera korolkow × Lonicera maackii and Lonicera korolkow × Lonicera tatarica No. 8 can generally be clustered with their parents. These results indicate that the analysis of pollen morphology characteristics, karyotype features, and floral and fruit pigment composition has value and significance for the study of systematic classification and relationships, and the conclusions of this study partially support morphological taxonomy.

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Anthocyanins in the Bracts of Curcuma Species and Relationship of the Species Based on Anthocyanin Composition

Cited by 7 publications

References 8 publications

The Chromosome-Scale Assembly of the Curcuma alismatifolia Genome Provides Insight Into Anthocyanin and Terpenoid Biosynthesis

The Chromosome-Scale Assembly of the Curcuma alismatifolia Genome Provides Insight Into Anthocyanin and Terpenoid Biosynthesis

The genomic and bulked segregant analysis of Curcuma alismatifolia revealed its diverse bract pigmentation

A taxonomic study on pollen morphology, karyotype features, floral and fruit pigment components of 13 taxa of Lonicera L.

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