Anthracnose field evaluation of sorghum germplasm from Botswana

Erpelding, John E.

doi:10.17221/34/2010-pps

Cited by 11 publications

(6 citation statements)

References 17 publications

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“…Certainly, the SAP encompasses the wide range of sorghum genetic diversity that has been exploited in breeding programs but it is not representative of the vast genetic diversity present in the NPGS collection. The NPGS sorghum germplasm collection includes 3044 accessions classified as the Guinea race originating from more than 20 countries, which exhibit a high frequency of anthracnose resistance (Cuevas et al, 2014b;Erpelding, 2011Erpelding, , 2012Erpelding and Prom, 2004). The expected assembly of a representative subset of Guinea germplasm by low-coverage resequencing combined with phenotypic characterization will be valuable for the discovery of new sources of disease resistance currently absent in the SAP.…”

Section: Discussionmentioning

confidence: 99%

Genome‐Wide Association Mapping of Anthracnose (Colletotrichum sublineolum) Resistance in the U.S. Sorghum Association Panel

et al. 2018

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The productivity and profitability of sorghum [ (L.) Moench] is reduced by susceptibility to fungal diseases, such as anthracnose ( P. Henn.). A limited number of resistant accessions are present in the temperate-adapted germplasm; other exotic sources of resistance are not currently available for breeding programs. Among 335 accessions available to breeders from a previously genotyped sorghum association panel (SAP), we found that 75 were resistant to anthracnose. A phylogenetic analysis of these accessions showed high genetic diversity and multiple resistance sources. Genome-wide association scans (GWAS) were conducted using 268,289 single-nucleotide polymorphisms to identify loci associated with anthracnose resistance. Using logistic regressions for binary measures of resistance responses, we identified three loci within a region on chromosome 5 that have been previously associated with three sources of anthracnose resistance. A GWAS limited to Caudatum germplasm identified an association with a region on chromosome 1 and with the same previous region on chromosome 5. Candidate genes within these loci were related to R-gene families, signaling cascades, and transcriptional reprogramming, suggesting that the resistance response is controlled by multiple defense mechanisms. The strategic integration of exotic resistant germplasm into the SAP is needed to identify additional rare resistance alleles via GWAS.

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Section: Discussionmentioning

confidence: 99%

Genome‐Wide Association Mapping of Anthracnose (Colletotrichum sublineolum) Resistance in the U.S. Sorghum Association Panel

et al. 2018

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“…Several sources of anthracnose resistance have been identified in tropical and temperate-adapted germplasm. The screening of the U.S. National Plant Germplasm System (NPGS) sorghum germplasm collection resulted in multiple sources of resistance being identified Erpelding 2011;Prom et al 2011;Erpelding 2012;Cuevas et al 2014b;Cuevas et al 2016). Most of these resistance sources are, however, tropical germplasm that cannot be integrated into U.S. sorghum breeding programs without conversion through introgression of photoperiod insensitivity using dwarfing and early maturity genes (Thurber et al 2013).…”

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confidence: 99%

Genomic Dissection of Anthracnose (Colletotrichum sublineolum) Resistance Response in Sorghum Differential Line SC112-14

Cruet-Burgos

Cuevas

Prom

et al. 2020

G3 Genes|Genomes|Genetics

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Sorghum production is expanding to warmer and more humid regions where its production is being limited by multiple fungal pathogens. Anthracnose, caused by Colletotrichum sublineolum, is one of the major diseases in these regions, where it can cause yield losses of both grain and biomass. In this study, 114 recombinant inbred lines (RILs) derived from resistant sorghum line SC112-14 were evaluated at four distinct geographic locations in the United States for response to anthracnose. A genome scan using a high-density linkage map of 3,838 single nucleotide polymorphisms (SNPs) detected two loci at 5.25 and 1.18 Mb on chromosomes 5 and 6, respectively, that explain up to 59% and 44% of the observed phenotypic variation. A bin-mapping approach using a subset of 31 highly informative RILs was employed to determine the disease response to inoculation with ten anthracnose pathotypes in the greenhouse. A genome scan showed that the 5.25 Mb region on chromosome 5 is associated with a resistance response to nine pathotypes. Five SNP markers were developed and used to fine map the locus on chromosome 5 by evaluating 1,500 segregating F2:3 progenies. Based on the genotypic and phenotypic analyses of 11 recombinants, the locus was narrowed down to a 470-kb genomic region. Following a genome-wide association study based on 574 accessions previously phenotyped and genotyped, the resistance locus was delimited to a 34-kb genomic interval with five candidate genes. All five candidate genes encode proteins associated with plant immune systems, suggesting they may act in synergy in the resistance response.

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“…However, the continued use of these limited sources of resistance in the United States coupled with the rapid evolution of fungal pathogens could lead to the collapse of resistance genes (Mcdonald and Linde 2002). Other sources of resistance have been identified in tropical (Erpelding and Prom 2004; Erpelding 2011; Erpelding 2012; Cuevas et al 2014b; Cuevas et al 2016; Cuevas et al 2018b) and temperate-adapted germplasm (Prom et al 2016; Cuevas et al 2018a). However, inheritance studies are lacking for most of these resistance sources, limiting the identification of new resistance loci for temporal deployment strategies to increase the durability of resistance sources.…”

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confidence: 99%

Genome-Wide Association Mapping of Anthracnose (Colletotrichum sublineolum) Resistance in NPGS Ethiopian Sorghum Germplasm

Cuevas

Prom

Cruet-Burgos

2019

G3 Genes|Genomes|Genetics

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The National Plant Germplasm System (NPGS) Ethiopian sorghum [ Sorghum bicolor (L.) Moench] collection of the United States is an important genetic resource for sorghum improvement. Anthracnose ( Colletotrichum sublineolum ) is one of the most harmful fungal diseases in humid sorghum production regions. Although multiple resistance sources have been identified in temperate-adapted germplasm in the Sorghum Association Panel (SAP), these resistance loci explain a limited portion of the total variation, and sources of resistance from tropical germplasm are not available for breeding programs at temperate regions. Using a core set of 335 previously genotyped NPGS Ethiopian accessions, we identified 169 accessions resistant to anthracnose. To identify resistance loci, we merged the genotypic and anthracnose response data for both NPGS Ethiopian germplasm and the SAP and performed genome-wide association scans using 219,037 single nucleotide polymorphisms and 617 accessions. The integrated data set enabled the detection of a locus on chromosome 9 present in the SAP at a low frequency. The locus explains a limited portion of the observed phenotypic variation ( r 2 = 0.31), suggesting the presence of other resistance loci. The locus in chromosome 9 was constituted by three R genes clustered within a 47-kb region. The presence of multiple sources of resistance in NPGS Ethiopian germplasm and SAP requires the inclusion of other resistance response evaluation that could revealed others low frequency resistance alleles in the panel.

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Anthracnose field evaluation of sorghum germplasm from Botswana

Abstract: Erpelding J.E. (2011): Anthracnose field evaluation of sorghum germplasm from Botswana. Plant Protect. Sci., 47: 149-156.

Cited by 11 publications

References 17 publications

Genome‐Wide Association Mapping of Anthracnose (Colletotrichum sublineolum) Resistance in the U.S. Sorghum Association Panel

Genome‐Wide Association Mapping of Anthracnose (Colletotrichum sublineolum) Resistance in the U.S. Sorghum Association Panel

Genomic Dissection of Anthracnose (Colletotrichum sublineolum) Resistance Response in Sorghum Differential Line SC112-14

Genome-Wide Association Mapping of Anthracnose (Colletotrichum sublineolum) Resistance in NPGS Ethiopian Sorghum Germplasm

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