Anthracyclines: recent developments in their separation and quantitation

Zagotto, Giuseppe; Gatto, Barbara; Moro, Stefano; Sissi, Claudia; Palumbo, Manlio

doi:10.1016/s0378-4347(01)00346-2

Cited by 58 publications

(38 citation statements)

References 36 publications

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“…More than a decade has past since the publication of the last comprehensive reviews covering determination of anthracyclines [15][16][17]. Given the new developments in the field -amongst which the use of tandem mass spectrometry-the aim of the current review is to bundle chromatographic strategies, new insights and developments for the detection of anthracyclines in biological matrices.…”

Section: Introductionmentioning

confidence: 99%

Quantitative liquid chromatographic analysis of anthracyclines in biological fluids

Maudens

Stove

Lambert

2011

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

Quantitative liquid chromatographic analysis of anthracyclines in biological fluids

Maudens

Stove

Lambert

2011

Journal of Chromatography B

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“…The anthracycline doxorubicin has been in clinical use for several decades and is still among the most widely used chemotherapeutic agents for treatment of a variety of neoplasms (Weiss, 1992;Lothstein et al, 2001;Zagotto et al, 2001). Despite many years of research in developing new and better anthracyclines, little or no change in the molecular structure of doxorubicin made it to the clinic.…”

mentioning

confidence: 99%

Coformulated N-Octanoyl-glucosylceramide Improves Cellular Delivery and Cytotoxicity of Liposomal Doxorubicin

Veldman¹,

Koning²,

Hell³

et al. 2005

The Journal of Pharmacology and Experimental Therapeutics

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The anticancer agent doxorubicin is in certain cases administered as a long-circulating liposomal formulation. Due to angiogenesis-related structural abnormalities in the endothelial lining of many neoplasms, these complexes tend to extravasate and accumulate in the tumor stroma. However, delivery of doxorubicin is still not optimal since liposomes are not taken up directly by tumor cells. Instead, doxorubicin is gradually released into the interstitial space, and the subsequent uptake by surrounding cells is a limiting step in the delivery process. We recently demonstrated that plasma membrane-inserted shortchain sphingomyelin facilitates the cellular uptake of free doxorubicin. Here, we report that N-octanoyl-glucosylceramide acts equally potent but is itself less toxic. When coformulated with liposomal doxorubicin, this short-chain glycosphingolipid administered to cultured A431 epidermoid carcinoma cells led to superior (up to 4-fold) cellular doxorubicin accumulation and cytotoxicity, compared with control doxorubicin liposomes. These results were fully reproducible when N-octanoyl-glucosylceramide was postinserted into Caelyx, a commercial liposomal doxorubicin preparation. The doxorubicin-potentiating effect of N-octanoyl-glucosylceramide-enriched liposomes proved relatively insensitive to high serum concentrations, indicating that in vivo application is a feasible option. N-Octanoyl-glucosylceramide enrichment might thus represent a major improvement of conventional liposomal doxorubicin formulations.

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“…DM is an anthracycline antibiotic that acts against a variety of human cancers (Danesi et al 2002). However, its therapeutic use is limited by its unique cardiotoxicity, and the pharmacokinetics of the drug in physiological fluids has been extensively studied by specific and sensitive assays (Zagotto et al 2001). The detailed conditions for establishing immunocytochemistry for drugs have not yet been elucidated.…”

Section: Discussionmentioning

confidence: 99%

Immunocytochemistry for Drugs Containing an Aliphatic Primary Amino Group in the Molecule, Anticancer Antibiotic Daunomycin as a Model

Fujiwara

Takatsu

Tsukamoto

2005

J Histochem Cytochem.

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S U M M A R Y An immunocytochemistry (ICC) for the anticancer antibiotic daunomycin (DM)was developed using a combination of anti-DM serum produced against N -(gamma-maleimidobutyryloxy)succinimide (GMBS)-conjugated DM, and DM-uptake human melanoma BD cells. The antiserum was demonstrated to be specific for DM and the structurally related analogs adriamicin and epirubicin by an ICC model system of the enzyme immunoassay (EIA) using glutaraldehyde (GA)-conjugated DM as a solid phase antigen. No cross-reaction occurred with any of the other antibiotics tested such as bleomycin, pepleomycin, and mitomycin C. Successful DM ICC required a series of processes prior to the immunocytochemical reaction: the cells were first fixed with GA, then reduced with NaBH 4 , treated with hydrochloric acid, and finally digested with protease. The cell specimens were then subjected to immunoreaction with anti-DM serum followed by peroxidase-labeled goat anti-rabbit IgG/ Fab Ј , and in both immune reagents the detergent Triton X-100 was contained as well. The present ICC covering all these processes successfully stained for DM in the nucleus and in the perinuclear Golgi region of the cytoplasm of the BD cells, consistent with the results obtained by the DM autofluorescence method. This ICC was found to be three times as sensitive as the cytofluorometric method and applicable to the paraffin sections of the liver of rats 24 hr after an IV injection of DM. The principle used in the present study for developing DM ICC might be applied to other drugs containing the primary amino group(s) in the molecule. Thus, these ICCs for drugs are direct, precise and easy new methods that should have potential for pharmacology and toxicology studies of drugs, revealing the localization of a drug in cells and tissues.

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Anthracyclines: recent developments in their separation and quantitation

Cited by 58 publications

References 36 publications

Quantitative liquid chromatographic analysis of anthracyclines in biological fluids

Quantitative liquid chromatographic analysis of anthracyclines in biological fluids

Coformulated N-Octanoyl-glucosylceramide Improves Cellular Delivery and Cytotoxicity of Liposomal Doxorubicin

Immunocytochemistry for Drugs Containing an Aliphatic Primary Amino Group in the Molecule, Anticancer Antibiotic Daunomycin as a Model

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